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[194263_x_at]

Description AV184300_rc /REP_DB=TREMBL Accession /GB=AV184300 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk662f12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_highest_nonorm > 17      6grp_total > 22      4grp_total > 24      6grp_highest_sub > 02      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4559
1 2 3 4 range
A 10680.8
(-3)
P 		12903
(1.4)
P 		13717
(3)
P 		11561.5
(-1.3)
P 		3036
B 11254
(-3)
P 		11318.2
(-2.8)
P 		12636.6
(0.9)
P 		13355.2
(3)
P 		2101
C 9679.5
(-3)
P 		10505.7
(-1.9)
P 		11403.7
(-0.7)
P 		14238.9
(3)
P 		4559
avr 10538.1
(-3) 		11575.6
(-0.5) 		12585.8
(1.9) 		13051.9
(3) 		2514

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7876
1 2 3 4 5 6 range
A 13302.5
(1.5)
P 		10462.3
(-0.7)
P 		15117.1
(3)
P 		9542.8
(-1.5)
P 		9991.4
(-1.1)
P 		7633.1
(-3)
P 		7484
B 9800
(-0.8)
P 		12980.6
(3)
P 		10487.9
(0)
P 		10128.3
(-0.4)
P 		8157.4
(-2.8)
P 		7970.3
(-3)
P 		5010
C 9768.7
(-0.5)
P 		13238.1
(3)
P 		10420.2
(0.2)
P 		8610.6
(-1.6)
P 		9295
(-0.9)
P 		7240.8
(-3)
P 		5997
avr 10957.1
(1.3) 		12227
(3) 		12008.4
(2.7) 		9427.2
(-0.6) 		9147.9
(-1) 		7614.7
(-3) 		4612
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2282
1 2 3 4 range
A 3888.9
(3)
P 		2800.9
(0)
P 		3585.9
(2.2)
P 		1692.4
(-3)
P 		2197
B 3588.8
(3)
P 		2795.3
(-3)
P 		2928.2
(-2)
P 		2846
(-2.6)
P 		794
C 3204.2
(0.1)
P 		3020.5
(-0.6)
P 		3974.5
(3)
P 		2400.9
(-3)
P 		1574
avr 3560.6
(3) 		2872.2
(-0.3) 		3496.2
(2.7) 		2313.1
(-3) 		1248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2589
1 2 3 4 5 6 range
A 2452.2
(-1.1)
P 		3720.9
(3)
P 		3292.2
(1.6)
P 		2420.3
(-1.2)
P 		3015
(0.7)
P 		1858.4
(-3)
P 		1863
B 3409.9
(-0.1)
P 		4320.7
(3)
P 		2546.8
(-3)
P 		2682.6
(-2.5)
P 		3314.2
(-0.4)
P 		2972.1
(-1.6)
P 		1774
C 3620.9
(1.9)
P 		4039.4
(3)
P 		2652.1
(-0.6)
P 		1731.6
(-3)
P 		3007.7
(0.3)
P 		2423.9
(-1.2)
P 		2308
avr 3161
(0) 		4027
(3) 		2830.4
(-1.1) 		2278.2
(-3) 		3112.3
(-0.1) 		2418.1
(-2.5) 		1749
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194263_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22239
Sequence Type Exemplar sequence
Representative Public ID AV184300 NCBI
Target Description AV184300_rc /REP_DB=TREMBL Accession /GB=AV184300 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk662f12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076922(11), NM_182443(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076922 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-4) (eft-4) mRNA, complete cds. 11/11 None
NM_182443 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-4) (eft-4) mRNA, complete cds. 11/11 None
R03G5.1c.1 ENSEMBL cdna:known chromosome:CEL140:X:7823589:7825708:1 gene:R03G5.1 11/11 None
R03G5.1c.2 ENSEMBL cdna:known chromosome:CEL140:X:7823593:7825708:1 gene:R03G5.1 11/11 None
R03G5.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:7823593:7825699:1 gene:R03G5.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
R03G5.1a.3 3/11 Cross Hyb Matching Probes None
R03G5.1a.2 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181044 4 2 NM_076922 192129_at (11), 194263_x_at (11)
NM_182443 192129_at (11), 194263_x_at (11)
NM_182444 192129_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7825332-7825682(+) 100.0 100.0
Public Domain and Genome References
Ensembl R03G5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81688 Elongation factor protein 3 [Caenorhabditis elegans] gb|AAA96068.1| Elongation factor protein 4, isoform a [Caenorhabditis elegans] ref|NP_509323.1| Elongation FacTor family member (eft-4) [Caenorhabditis elegans] ref|NP_498520.1| Elongation FacTor family member (eft-3) [Caenorhabditis elegans] sp|P53013|EF1A_CAEEL Elongation factor 1-alpha (EF-1-alpha) 0.0
blast CAE70307 Hypothetical protein CBG16828 [Caenorhabditis briggsae] emb|CAE70057.1| Hypothetical protein CBG16491 [Caenorhabditis briggsae] emb|CAE56763.1| Hypothetical protein CBG24566 [Caenorhabditis briggsae] 0.0
blast P27592 Elongation factor 1-alpha (EF-1-alpha) gb|AAA29416.1| elongation factor 0.0
blast AAO21383 Elongation factor protein 4, isoform c [Caenorhabditis elegans] ref|NP_872243.1| Elongation FacTor family member (eft-4) [Caenorhabditis elegans] 1.0E-133
blast AAY56745 elongation factor 1-alpha [Caenorhabditis remanei] 8.0E-81
Protein Domains
Database ID Description E-Value
scop a.4.5.Transcription factor MotA, activation domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Transcription factor MotA, activation domain 1.20000004768372
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 3.5E-81
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-25
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.1E-112
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 6.4E-57
Sequence
Target Sequence 
>C. ELEGANS:194263_X_AT
gtccacgcagccttttgtacaaaagtcgtgtgtttgggcaacacttttttgtttcaaaaa
accgtccatccaaaaaaaccttcgctacgtatttattaaggctcgttatgttttccccaa
aacaaagcggtcttcatcaaaaataataaatacagttacttgtaccatttgatcatcact
aatgactccccccgacccccccccccaatcctcccactccaaaaatcctttaaaaactgt
tatgttaatatgtcatgcattgttttgtcgcattctgtcatccatcgttttgtttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCACGCAGCCTTTTGTACAAAAG 61 477 33 Antisense
AAGTCGTGTGTTTGGGCAACACTTT 414 155 55 Antisense
TCCATCCAAAAAAACCTTCGCTACG 703 589 97 Antisense
TTCGCTACGTATTTATTAAGGCTCG 364 683 113 Antisense
TTCCCCAAAACAAAGCGGTCTTCAT 637 695 145 Antisense
AACAAAGCGGTCTTCATCAAAAATA 335 133 153 Antisense
ATACAGTTACTTGTACCATTTGATC 217 25 182 Antisense
TTTGATCATCACTAATGACTCCCCC 401 667 200 Antisense
AATATGTCATGCATTGTTTTGTCGC 379 173 279 Antisense
CATTGTTTTGTCGCATTCTGTCATC 631 217 290 Antisense
TTCTGTCATCCATCGTTTTGTTTCG 654 693 305 Antisense
Sequence Source Affymetrix Proprietary Database