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[194255_x_at]

Description C68743 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F22A3.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C68743 /WB_GENE_ID=F22A3.6 /WP=CE04442 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk303e11 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 42      6grp_highest_nonorm > 24      6grp_total > 21      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9612
1 2 3 4 range
A 5525.9
(0.6)
P 		8791.4
(2.7)
P 		9216.5
(3)
P 		93.2
(-3)
A 		9123
B 6048.3
(0.7)
P 		6307.4
(0.9)
P 		9690.4
(3)
P 		78.4
(-3)
A 		9612
C 5451.9
(0.9)
P 		6539
(1.6)
P 		8448.2
(3)
P 		82.4
(-3)
A 		8366
avr 5675.4
(0.7) 		7212.6
(1.7) 		9118.4
(3) 		84.7
(-3) 		9034

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4152
1 2 3 4 5 6 range
A 3220.1
(-1)
P 		4812.3
(2.2)
P 		5227.3
(3)
P 		3150.2
(-1.1)
P 		2827.2
(-1.8)
P 		2205.8
(-3)
P 		3022
B 2411.3
(-2.5)
P 		4395
(0.4)
P 		6206.9
(3)
P 		2691
(-2.1)
P 		2908.6
(-1.8)
P 		2054.5
(-3)
P 		4152
C 2478.1
(-2.8)
P 		4335.2
(0.8)
P 		5497.6
(3)
P 		2758.2
(-2.3)
P 		2813.7
(-2.2)
P 		2376.5
(-3)
P 		3121
avr 2703.2
(-2.1) 		4514.2
(1) 		5643.9
(3) 		2866.5
(-1.9) 		2849.8
(-1.9) 		2212.3
(-3) 		3432
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2931
1 2 3 4 range
A 2012
(2)
P 		1908.4
(1.7)
P 		2409.4
(3)
P 		13.6
(-3)
A 		2396
B 1928.8
(2.1)
P 		1557.8
(1.1)
P 		2245.5
(3)
P 		16.7
(-3)
A 		2229
C 1804.7
(0.7)
P 		1880
(0.8)
P 		2944.5
(3)
P 		13.9
(-3)
A 		2931
avr 1915.2
(1.5) 		1782.1
(1.2) 		2533.1
(3) 		14.7
(-3) 		2518

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1174
1 2 3 4 5 6 range
A 593.6
(-2.7)
P 		1711.5
(3)
P 		1138.4
(0.1)
P 		799
(-1.7)
P 		853.1
(-1.4)
P 		537.1
(-3)
P 		1174
B 839
(-2)
P 		1462.9
(2.7)
P 		1507.2
(3)
P 		712.8
(-3)
P 		1181.7
(0.5)
P 		766.1
(-2.6)
P 		794
C 918.5
(-0.4)
P 		1322.8
(2.5)
P 		1399.2
(3)
P 		554.7
(-3)
P 		910.5
(-0.5)
P 		795.6
(-1.3)
P 		845
avr 783.7
(-2.3) 		1499.1
(3) 		1348.3
(1.9) 		688.8
(-3) 		981.8
(-0.8) 		699.6
(-2.9) 		810
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194255_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25835
Sequence Type Exemplar sequence
Representative Public ID C68743 NCBI
Target Description C68743 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F22A3.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C68743 /WB_GENE_ID=F22A3.6 /WP=CE04442 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk303e11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029423(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029423 NCBI Caenorhabditis elegans F22A3.6a (F22A3.6) mRNA, complete cds. 10/11 None
GENEFINDER00000004717 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6532599:6536066:-1 9/11 None
F22A3.6a ENSEMBL cdna:known chromosome:CEL140:X:6533504:6536732:-1 gene:F22A3.6 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029424 4/11 Cross Hyb Matching Probes None
SNAP00000004714 3/11 Cross Hyb Matching Probes None
F22A3.6b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180928 2 1 NM_001029423 172400_x_at (11), 173051_s_at (11), 194255_x_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6533504-6536733(-) 100.0 100.0
Public Domain and Genome References
Ensembl F22A3.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024594 F22A3.6a [Caenorhabditis elegans] gb|AAA83197.1| Hypothetical protein F22A3.6a [Caenorhabditis elegans] 4.0E-84
blast CAE65750 Hypothetical protein CBG10836 [Caenorhabditis briggsae] 2.0E-77
blast NP_001024594 F22A3.6a [Caenorhabditis elegans] gb|AAA83197.1| Hypothetical protein F22A3.6a [Caenorhabditis elegans] 2.0E-74
blast NP_500206 C45G7.3 [Caenorhabditis elegans] gb|AAC19179.1| Hypothetical protein C45G7.3 [Caenorhabditis elegans] 4.0E-72
blast CAE65750 Hypothetical protein CBG10836 [Caenorhabditis briggsae] 1.0E-67
blast NP_500206 C45G7.3 [Caenorhabditis elegans] gb|AAC19179.1| Hypothetical protein C45G7.3 [Caenorhabditis elegans] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR008597 EMBL-EBI Destabilase 1.2E-71
Pfam IPR008597 EMBL-EBI Destabilase 2.6E-69
Sequence
Target Sequence 
>C. ELEGANS:194255_X_AT
tatgtttgtcaaatcgcttctgctcctttctgtcgctattgcgtacgtttccgccgactg
cctccactgcatctgcatgagagagtcaggatgcaaaccaatcggttgccacatggacgt
tggatccctctcctgcggatactatcagatcaagattggatactatgaagactgcggaca
accaacaaagaaggctggagagactactgaagctgcctggaagagatgtgctgatgatct
taactgcgctaccacttgcgtcgagaactactacaaccgctacaagtctcagtgcaatgg
acttggaatgggagcttgccagatcatgtcccgtaaccacaacggaggaccacgtggatg
ccacaacgccaacaccttggcttact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGTTTGTCAAATCGCTTCTGCTC 205 657 13 Antisense
CTGTCGCTATTGCGTACGTTTCCGC 406 239 42 Antisense
TGCCTCCACTGCATCTGCATGAGAG 339 585 71 Antisense
GTTGCCACATGGACGTTGGATCCCT 536 439 117 Antisense
GAGAGACTACTGAAGCTGCCTGGAA 317 395 210 Antisense
GTGCTGATGATCTTAACTGCGCTAC 162 479 240 Antisense
CGTCGAGAACTACTACAACCGCTAC 384 243 271 Antisense
CAACCGCTACAAGTCTCAGTGCAAT 539 187 286 Antisense
TGGGAGCTTGCCAGATCATGTCCCG 176 555 321 Antisense
CAACGGAGGACCACGTGGATGCCAC 199 187 352 Antisense
CACAACGCCAACACCTTGGCTTACT 205 195 374 Antisense
Sequence Source Affymetrix Proprietary Database