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[194229_x_at]

Description g385198 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=ZC416.8A /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=ZC416.8A /WP=CE17307 /CHR=4 /FEA=Genomic Cluster /GEN=unc-17 /DEF=acetylcholine transporter; amine transporter
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 16      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 467
1 2 3 4 range
A 626.6
(3)
P 		428.7
(0)
P 		225.6
(-3)
P 		309.1
(-1.8)
P 		401
B 690.3
(3)
P 		375.8
(-1.2)
P 		239.7
(-3)
P 		317.8
(-2)
P 		451
C 693
(3)
P 		462.1
(-0.2)
P 		262.2
(-3)
P 		288.8
(-2.6)
P 		431
avr 670
(3) 		422.2
(-0.5) 		242.5
(-3) 		305.2
(-2.1) 		428

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 585
1 2 3 4 5 6 range
A 675.2
(3)
P 		365.7
(-0.4)
P 		182.2
(-2.3)
P 		122.1
(-3)
P 		189.3
(-2.3)
P 		187.9
(-2.3)
P 		553
B 631.4
(3)
P 		424.3
(0.6)
P 		176.3
(-2.2)
P 		191.5
(-2.1)
P 		109.2
(-3)
P 		127
(-2.8)
P 		522
C 624.8
(3)
P 		402.4
(0.5)
P 		176.4
(-2)
P 		131.5
(-2.5)
P 		183.9
(-2)
P 		90.6
(-3)
P 		534
avr 643.8
(3) 		397.5
(0.1) 		178.3
(-2.5) 		148.4
(-2.8) 		160.8
(-2.7) 		135.2
(-3) 		509
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 228.2
(3)
P 		93.1
(-1.4)
P 		59
(-2.5)
P 		45.2
(-3)
P 		183
B 220.1
(3)
P 		92.8
(-1.6)
P 		55.6
(-3)
P 		67.7
(-2.6)
P 		165
C 229.4
(3)
P 		132.9
(-0.2)
P 		91.4
(-1.6)
P 		48.7
(-3)
P 		181
avr 225.9
(3) 		106.3
(-1.2) 		68.7
(-2.5) 		53.9
(-3) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 124.5
(2.7)
P 		130.1
(3)
P 		39.7
(-2.5)
P 		31
(-3)
P 		57.1
(-1.4)
P 		45.7
(-2.1)
P 		99
B 219.7
(3)
P 		141.2
(0.3)
P 		42.8
(-3)
P 		50.7
(-2.7)
P 		44.4
(-2.9)
P 		47.4
(-2.8)
P 		177
C 231.6
(3)
P 		122.8
(-0.2)
P 		44.9
(-2.5)
P 		26.4
(-3)
P 		59.5
(-2)
P 		30.3
(-2.9)
P 		205
avr 191.9
(3) 		131.4
(0.7) 		42.5
(-2.7) 		36
(-3) 		53.7
(-2.3) 		41.1
(-2.8) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194229_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24882
Sequence Type Exemplar sequence
Representative Public ID 385198 NCBI
Target Description g385198 /REP_DB=GenBank Identifier /5_PRIME_EXT_ID=ZC416.8A /5_PRIME_EXT_DB=WormBase Gene ID /WB_GENE_ID=ZC416.8A /WP=CE17307 /CHR=4 /FEA=Genomic Cluster /GEN=unc-17 /DEF=acetylcholine transporter; amine transporter
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028431(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028431 NCBI Caenorhabditis elegans UNCoordinated family member (unc-17) (unc-17) mRNA, complete cds. 11/11 None
SNAP00000026351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3614049:3632550:-1 10/11 None
GENEFINDER00000026358 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3618495:3627846:-1 10/11 None
ZC416.8a ENSEMBL cdna:known chromosome:CEL140:IV:3618250:3624692:-1 gene:ZC416.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177124 2 1 NM_001028431 172038_x_at (11), 192428_s_at (11), 194229_x_at (11)
NM_001028432 172037_x_at (11), 188870_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3618373-3623473(-) 96.47 100.0
Public Domain and Genome References
Ensembl ZC416.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023602 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88371.1| Uncoordinated protein 17, isoform a [Caenorhabditis elegans] sp|P34711|UNC17_CAEEL Vesicular acetylcholine transporter unc-17 (Uncoordinated protein 17) gb|AAC14456.1| acetylcholine transporter [Caenorhabditis elegans] gb|AAC13764.1| UNC-17 0.0
blast CAE63770 Hypothetical protein CBG08307 [Caenorhabditis briggsae] 0.0
blast NP_001023603 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88370.1| Abnormal choline acetyltransferase protein 1, isoform b [Caenorhabditis elegans] sp|P32756|CLAT_CAEEL Choline O-acetyltransferase (CHOACTase) (Choline acetylase) (ChAT) gb|AAA53660.1| choline acetyltransferase gb|AAA53659.1| choline acetyltransferase 0.0
blast CAE63768 Hypothetical protein CBG08305 [Caenorhabditis briggsae] 0.0
blast EAL27592 GA17179-PA [Drosophila pseudoobscura] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023602.1 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
ZC416.8a 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
SNAP00000026351 11 39-61,134-156,163-185,200-222,229-247,267-289,309-331,341-361,368-390,405-427,432-454
GENEFINDER00000026358 10 115-137,210-232,271-293,303-325,346-368,383-405,418-440,444-466,487-504,508-530
Sequence
Target Sequence 
>C. ELEGANS:194229_X_AT
gcttcggaatcgccttgatcgacacctcgctcctcccgatgcttggacaccttgtggaca
cccgccacgtttcggtttacggatccgtctacgcgatcgctgacatttcgtactcgttgg
cttacgcctttggacccatcattgccggatggattgtgaccaactggggattcaccgccc
tcaacatcataatcttcgccaccaacgtcacctacgccccagttttattcctactgcgga
aagttcactcctacgacaccctcggcgccaagggtgacaccgccgaaatgacacagctca
actcatcggctccagctggcggatacaatgggaagcctgaagcaactacagcggaatcct
atcaaggatgggaagatcagcagtcgtaccagaatcaggcacagatcccgaaccacgccg
tgtcgttccaggattcaaggccccaagccgaattccccgccggatatgacccactgaatc
cgcagtggtagtcgtagatttggatctctgaatatgtttattctaccaagtgcaatggcc
aaaatgactagcctagattgctttatttccttttttctttcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCGGAATCGCCTTGATCGACAC 396 303 1122 Antisense
GATGCTTGGACACCTTGTGGACACC 115 413 1159 Antisense
GTTTACGGATCCGTCTACGCGATCG 347 447 1196 Antisense
ATCGCTGACATTTCGTACTCGTTGG 622 35 1217 Antisense
TACTCGTTGGCTTACGCCTTTGGAC 129 647 1232 Antisense
TGCGGAAAGTTCACTCCTACGACAC 706 581 1356 Antisense
TGACACCGCCGAAATGACACAGCTC 692 571 1396 Antisense
GAAGCAACTACAGCGGAATCCTATC 210 339 1460 Antisense
CAGAATCAGGCACAGATCCCGAACC 437 203 1511 Antisense
CGCCGTGTCGTTCCAGGATTCAAGG 183 257 1537 Antisense
TGCTTTATTTCCTTTTTTCTTTCAG 39 579 1680 Antisense
Sequence Source GenBank