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[194226_x_at]

Description C48277 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K01A2.8A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C48277 /WB_GENE_ID=K01A2.8A /WP=CE19516 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk461g9 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 23      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 42      6grp_total > 03      4grp_total > 52      6grp_high1_sub > 02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 944
1 2 3 4 range
A 1056.2
(3)
P 		1016.4
(2.7)
P 		702.8
(0.4)
P 		229.7
(-3)
P 		827
B 892.1
(2.3)
P 		993.7
(3)
P 		684.2
(0.9)
P 		129.8
(-3)
P 		864
C 1073.3
(3)
P 		951.6
(2.2)
P 		616.5
(-0.1)
P 		186.2
(-3)
P 		887
avr 1007.2
(3) 		987.2
(2.9) 		667.8
(0.5) 		181.9
(-3) 		825

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 670
1 2 3 4 5 6 range
A 1119.4
(3)
P 		1049
(2.3)
P 		729.6
(-0.7)
P 		888.1
(0.8)
P 		827.8
(0.2)
P 		485.3
(-3)
P 		634
B 965.1
(2.4)
P 		1002.9
(2.8)
P 		600.3
(-1.4)
P 		1025.6
(3)
P 		728.6
(-0.1)
P 		449.8
(-3)
P 		576
C 813.4
(1.1)
P 		957.9
(3)
P 		794.5
(0.9)
P 		889.8
(2.1)
P 		614.1
(-1.5)
P 		498.9
(-3)
P 		459
avr 966
(2.6) 		1003.3
(3) 		708.1
(-0.4) 		934.5
(2.2) 		723.5
(-0.2) 		478
(-3) 		525
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 357
1 2 3 4 range
A 384.6
(3)
P 		220.6
(0.2)
P 		183.7
(-0.4)
P 		33.6
(-3)
P 		351
B 284.5
(3)
P 		245.4
(2.1)
P 		158.6
(0.1)
P 		27.7
(-3)
P 		257
C 355.3
(3)
P 		273.6
(1.5)
P 		214.9
(0.4)
P 		31.4
(-3)
P 		324
avr 341.5
(3) 		246.5
(1.2) 		185.7
(-0) 		30.9
(-3) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 206.3
(-0.9)
P 		373.1
(3)
P 		158.9
(-2)
P 		225.2
(-0.5)
P 		249.8
(0.1)
P 		118.1
(-3)
P 		255
B 335.8
(3)
P 		333.8
(2.9)
P 		145.8
(-3)
P 		271.6
(1)
P 		296
(1.7)
P 		167.7
(-2.3)
P 		190
C 301.5
(3)
P 		292.3
(2.6)
P 		202.2
(-1.4)
P 		178.9
(-2.5)
P 		198.7
(-1.6)
P 		167
(-3)
P 		135
avr 281.2
(1.3) 		333.1
(3) 		169
(-2.4) 		225.2
(-0.6) 		248.2
(0.2) 		150.9
(-3) 		182
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194226_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23747
Sequence Type Exemplar sequence
Representative Public ID C48277 NCBI
Target Description C48277 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K01A2.8A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C48277 /WB_GENE_ID=K01A2.8A /WP=CE19516 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk461g9 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061303(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061303 NCBI Caenorhabditis elegans K01A2.8a (K01A2.8) mRNA, complete cds. 9/11 A 
K01A2.8c ENSEMBL cdna:known chromosome:CEL140:II:309540:310978:-1 gene:K01A2.8 9/11 A 
K01A2.8a ENSEMBL cdna:known chromosome:CEL140:II:309554:311349:-1 gene:K01A2.8 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182229 7/11 Cross Hyb Matching Probes None
NM_061306 4/11 Cross Hyb Matching Probes A 
GENEFINDER00000016841 7/11 Cross Hyb Matching Probes None
GENEFINDER00000016851 4/11 Cross Hyb Matching Probes None
GENEFINDER00000016852 4/11 Cross Hyb Matching Probes None
K01A2.8b 7/11 Cross Hyb Matching Probes A 
K01A2.9 4/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173419 2 1 NM_061303 194188_x_at (11), 194226_x_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:309554-310697(-) 100.0 100.0
Public Domain and Genome References
Ensembl K01A2.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69501 Mirp k channel accessory subunit protein 2, isoform a [Caenorhabditis elegans] ref|NP_493704.1| K01A2.8a [Caenorhabditis elegans] 4.0E-66
blast AAQ96604 MiRP4 [Caenorhabditis elegans] 6.0E-65
blast AAZ94292 Mirp k channel accessory subunit protein 2, isoform d [Caenorhabditis elegans] gb|AAQ96603.1| MPS-3 [Caenorhabditis elegans] 2.0E-51
blast AAO38626 Mirp k channel accessory subunit protein 2, isoform c [Caenorhabditis elegans] ref|NP_872029.1| K01A2.8c [Caenorhabditis elegans] 4.0E-47
blast AAC69501 Mirp k channel accessory subunit protein 2, isoform a [Caenorhabditis elegans] ref|NP_493704.1| K01A2.8a [Caenorhabditis elegans] 4.0E-47
blast AAZ94292 Mirp k channel accessory subunit protein 2, isoform d [Caenorhabditis elegans] gb|AAQ96603.1| MPS-3 [Caenorhabditis elegans] 4.0E-47
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493704.1 4 29-51,66-88,101-123,133-155
K01A2.8c 3 20-42,55-74,84-106
K01A2.8a 4 29-51,66-88,101-123,133-155
Sequence
Target Sequence 
>C. ELEGANS:194226_X_AT
gataggaggcttgccatcgtatccttccacagtttatttatttactcttcctttgataaa
atgtctgaaagaaaaaagaatgtactcctgtccctgcactctctttcatctttaatccac
gtggcaatgttctatttcctattggtccaatggagtaaagccgataatccagttctcaca
cattttcaggttttctgcctggtgattattggaatcggatttttgattatcttgatctta
atattcagtatttttaaatccgaaatgaaaagtgactcacatgcaagacgattctttcat
agttggccactggtacatatcgccttatccaccgcctatcagctcttcacgcaaattgtg
atatctcattggaaaacttaccagatcccaatcctctactcatcctatgcaactaccgtc
tctgtttgcattcattta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAGGAGGCTTGCCATCGTATCCT 307 425 20 Antisense
CATCGTATCCTTCCACAGTTTATTT 597 211 34 Antisense
CTCTCTTTCATCTTTAATCCACGTG 71 231 118 Antisense
TCCACGTGGCAATGTTCTATTTCCT 251 587 135 Antisense
AAAGCCGATAATCCAGTTCTCACAC 293 125 176 Antisense
GTTCTCACACATTTTCAGGTTTTCT 384 443 191 Antisense
GACGATTCTTTCATAGTTGGCCACT 179 379 306 Antisense
GGCCACTGGTACATATCGCCTTATC 210 541 324 Antisense
CCTATCAGCTCTTCACGCAAATTGT 437 269 354 Antisense
TCTACTCATCCTATGCAACTACCGT 149 615 414 Antisense
TACCGTCTCTGTTTGCATTCATTTA 143 651 433 Antisense
Sequence Source Affymetrix Proprietary Database