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[194224_x_at]

Description C44630 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y56A3A.12A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C44630 /WB_GENE_ID=Y56A3A.12A /WP=CE24467 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk371e9 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 63      6grp_total > 60      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 717.7
(-3)
P 		769.7
(0.3)
P 		727.4
(-2.4)
P 		812.6
(3)
P 		95
B 696.6
(-0.2)
P 		628.4
(-3)
P 		678.1
(-1)
P 		775.8
(3)
P 		147
C 681.4
(-3)
P 		846.1
(1.2)
P 		682.2
(-3)
P 		916.1
(3)
P 		235
avr 698.6
(-2.9) 		748.1
(-0.7) 		695.9
(-3) 		834.8
(3) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 306
1 2 3 4 5 6 range
A 600.6
(-0.6)
P 		522.7
(-2.1)
P 		589
(-0.8)
P 		784.7
(3)
P 		573.3
(-1.1)
P 		478.5
(-3)
P 		306
B 614.4
(3)
P 		522.5
(-2.5)
P 		610.6
(2.8)
P 		552.5
(-0.7)
P 		578.1
(0.8)
P 		514.3
(-3)
P 		100
C 663.8
(3)
P 		546.1
(-1.2)
P 		663.1
(3)
P 		580
(0)
P 		493.8
(-3)
P 		562
(-0.6)
P 		170
avr 626.3
(2.4) 		530.4
(-2.4) 		620.9
(2.1) 		639.1
(3) 		548.4
(-1.5) 		518.3
(-3) 		121
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 261.3
(3)
P 		167.1
(-1)
P 		190.2
(0)
P 		118.9
(-3)
P 		142
B 222.1
(3)
P 		155.2
(-3)
P 		157.1
(-2.8)
P 		165.3
(-2.1)
P 		67
C 225.6
(1.8)
P 		243.3
(3)
P 		237.8
(2.6)
P 		154.5
(-3)
P 		89
avr 236.3
(3) 		188.5
(-0.2) 		195
(0.2) 		146.2
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 110.7
(-3)
P 		185.9
(2.1)
P 		128.3
(-1.8)
P 		199
(3)
P 		173
(1.2)
P 		116.5
(-2.6)
P 		88
B 213.8
(1.6)
P 		173.9
(-1.1)
P 		148.3
(-2.9)
P 		146.3
(-3)
P 		234.9
(3)
P 		191.8
(0.1)
P 		89
C 246.1
(3)
P 		166.6
(-0.7)
P 		168.8
(-0.6)
P 		116.6
(-3)
P 		159.8
(-1)
P 		188.1
(0.3)
P 		130
avr 190.2
(3) 		175.5
(0.9) 		148.5
(-3) 		154
(-2.2) 		189.2
(2.9) 		165.5
(-0.6) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194224_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24000
Sequence Type Exemplar sequence
Representative Public ID C44630 NCBI
Target Description C44630 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y56A3A.12A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C44630 /WB_GENE_ID=Y56A3A.12A /WP=CE24467 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk371e9 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067143(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067143 NCBI Caenorhabditis elegans Y56A3A.12b (Y56A3A.12) mRNA, complete cds. 10/11 A 
Y56A3A.12b.1 ENSEMBL cdna:known chromosome:CEL140:III:11899217:11906785:-1 gene:Y56A3A.12 10/11 None
Y56A3A.12b.2 ENSEMBL cdna:known chromosome:CEL140:III:11899217:11906787:-1 gene:Y56A3A.12 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067144 7/11 Cross Hyb Matching Probes A 
SNAP00000035194 5/11 Cross Hyb Matching Probes None
GENEFINDER00000035221 7/11 Cross Hyb Matching Probes None
Y56A3A.12a.2 7/11 Cross Hyb Matching Probes A 
Y56A3A.12a.1 7/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176620 2 1 NM_067143 194224_x_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11899277-11906750(-) 96.93 100.0
Public Domain and Genome References
Ensembl Y56A3A.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60525 Hypothetical protein Y56A3A.12b [Caenorhabditis elegans] ref|NP_499544.1| Y56A3A.12b [Caenorhabditis elegans] 0.0
blast CAB60524 Hypothetical protein Y56A3A.12a [Caenorhabditis elegans] ref|NP_499545.1| Y56A3A.12a [Caenorhabditis elegans] 0.0
blast CAE67735 Hypothetical protein CBG13310 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 1.7E-56
Pfam IPR000120 EMBL-EBI Amidase 3.0E-7
Sequence
Target Sequence 
>C. ELEGANS:194224_X_AT
gatgcattgatttgtccttcttttattgctccagctcaaccgttcgaatatccggctcaa
ctatcaaatggagcattcataaccggactattcaatatgcttgatgtcccagctggcgtc
gttccagtttccccagtcaaccagaaagacgttgatcaattgattgatggattctctacc
gaaggagatttgcttctgaaaaagcaacgagaagctgctcgaggcaccaccgggttgccg
aatgctgtacaggtcgtaactcttccgaattgtgaagaaatgtgccttcgtgtcatgaga
cttgttgaagagtcggctgaaggtgttcaacgacttcagtggagagttggagcatcggcg
gctccgattgatgtggagaacacgccggctggagttgtgagcagcttggagcatttcgaa
cgtgttaatcttcttcattagttgctatttataactggtttttcatgtggtgtcaggctg
tcccataacggcttgatctacgaaaaatgagggaattttttttttaattcccgcgattct
cggagatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCATTGATTTGTCCTTCTTTTA 357 413 1517 Antisense
TTCTTTTATTGCTCCAGCTCAACCG 373 689 1534 Antisense
GCTCAACCGTTCGAATATCCGGCTC 677 299 1550 Antisense
ACCACCGGGTTGCCGAATGCTGTAC 24 87 1742 Antisense
GCTGTACAGGTCGTAACTCTTCCGA 117 295 1760 Antisense
AATGTGCCTTCGTGTCATGAGACTT 580 163 1795 Antisense
GAACACGCCGGCTGGAGTTGTGAGC 272 351 1894 Antisense
GCAGCTTGGAGCATTTCGAACGTGT 491 313 1917 Antisense
TCATGTGGTGTCAGGCTGTCCCATA 324 623 1979 Antisense
CTGTCCCATAACGGCTTGATCTACG 225 239 1994 Antisense
TAATTCCCGCGATTCTCGGAGATCA 579 633 2041 Antisense
Sequence Source Affymetrix Proprietary Database