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[194220_x_at]

Description AV192198 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K04F10.4E /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV192198 /WB_GENE_ID=K04F10.4E /WP=CE11736 /CHR=1 /FEA=Genomic Cluster /GEN=bli-4 /DEF=endoprotease; Caenorhabditis elegans cDNA clone:yk602e5 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 46      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 74      6grp_total > 47      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 26      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 452.6
(-1.1)
P 		582.4
(3)
P 		527.6
(1.3)
P 		393.5
(-3)
P 		189
B 504.3
(3)
P 		497.2
(2.6)
P 		451.6
(0.3)
P 		386.8
(-3)
P 		118
C 539
(1.6)
P 		536.8
(1.5)
P 		581.3
(3)
P 		397.6
(-3)
P 		184
avr 498.6
(1.4) 		538.8
(3) 		520.2
(2.2) 		392.6
(-3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 381
1 2 3 4 5 6 range
A 620.3
(0.7)
P 		674.2
(2.2)
P 		484.4
(-3)
P 		703.7
(3)
P 		585.3
(-0.2)
P 		633
(1.1)
P 		219
B 628.3
(-2.2)
P 		636.5
(-1.8)
P 		659.1
(-0.9)
P 		754.3
(3)
P 		608.4
(-3)
P 		697.8
(0.7)
P 		146
C 865.1
(3)
P 		722.7
(0.1)
P 		675
(-0.8)
P 		708.7
(-0.1)
P 		565.7
(-3)
P 		771.3
(1.1)
P 		299
avr 704.6
(2.2) 		677.8
(1) 		606.2
(-2.1) 		722.2
(3) 		586.5
(-3) 		700.7
(2) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 164.8
(3)
P 		126.4
(0.9)
P 		137.9
(1.5)
P 		57.6
(-3)
P 		107
B 160.8
(3)
P 		122.8
(0.1)
P 		104.6
(-1.3)
P 		82.4
(-3)
P 		78
C 178.4
(1.9)
P 		154.3
(0.9)
P 		202.6
(3)
P 		67
(-3)
P 		136
avr 168
(3) 		134.5
(1) 		148.4
(1.8) 		69
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 114.3
(-2.6)
P 		239.8
(3)
P 		105.5
(-3)
P 		178.5
(0.3)
P 		176.6
(0.2)
P 		154.1
(-0.8)
P 		134
B 218.6
(0.5)
P 		211.9
(0.1)
P 		160
(-3)
P 		199.8
(-0.6)
P 		247.2
(2.2)
P 		260.2
(3)
P 		100
C 320.6
(3)
P 		220.5
(-0.4)
P 		171.8
(-2)
P 		142.5
(-3)
P 		183.1
(-1.6)
P 		258.2
(0.9)
P 		178
avr 217.8
(2.5) 		224.1
(3) 		145.8
(-3) 		173.6
(-0.9) 		202.3
(1.3) 		224.2
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194220_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22651
Sequence Type Exemplar sequence
Representative Public ID AV192198 NCBI
Target Description AV192198 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K04F10.4E /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV192198 /WB_GENE_ID=K04F10.4E /WP=CE11736 /CHR=1 /FEA=Genomic Cluster /GEN=bli-4 /DEF=endoprotease; Caenorhabditis elegans cDNA clone:yk602e5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026373, NM_001026371, NM_001026374, NM_001026369, NM_001026372, NM_001026370
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026370 NCBI Caenorhabditis elegans K04F10.4b (endoprotease) mRNA, complete cds. None
NM_001026372 NCBI Caenorhabditis elegans K04F10.4d (endoprotease) mRNA, complete cds. None
K04F10.4b ENSEMBL cdna:known chromosome:CEL140:I:6349479:6361740:1 gene:K04F10.4 A 
K04F10.4f ENSEMBL cdna:known chromosome:CEL140:I:6350572:6354300:1 gene:K04F10.4 A 
K04F10.4a.1 ENSEMBL cdna:known chromosome:CEL140:I:6350366:6356621:1 gene:K04F10.4 A 
K04F10.4a.2 ENSEMBL cdna:known chromosome:CEL140:I:6350366:6356599:1 gene:K04F10.4 A 
K04F10.4e ENSEMBL cdna:known chromosome:CEL140:I:6350572:6353982:1 gene:K04F10.4 A 
K04F10.4d ENSEMBL cdna:known chromosome:CEL140:I:6350572:6364813:1 gene:K04F10.4 A 
K04F10.4c ENSEMBL cdna:known chromosome:CEL140:I:6350572:6363567:1 gene:K04F10.4 A 
NM_001026369 NCBI Caenorhabditis elegans K04F10.4a (endoprotease) mRNA, complete cds. None
NM_001026374 NCBI Caenorhabditis elegans K04F10.4f (endoprotease) mRNA, complete cds. None
NM_001026371 NCBI Caenorhabditis elegans K04F10.4c (endoprotease) mRNA, complete cds. None
NM_001026373 NCBI Caenorhabditis elegans K04F10.4e (endoprotease) mRNA, complete cds. None
SNAP00000027815 ENSEMBL cdna:SNAP chromosome:CEL140:I:6350572:6353721:1 None
GENEFINDER00000027829 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6350572:6353721:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016565 1/11 Cross Hyb Matching Probes None
GENEFINDER00000016575 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6350501-6353912(+) 100.0 100.0
Public Domain and Genome References
Ensembl K04F10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96756 Blistered cuticle protein 4, isoform b [Caenorhabditis elegans] ref|NP_001021541.1| K04F10.4b [Caenorhabditis elegans] pir||F87803 protein bli-4B [imported] - Caenorhabditis elegans 0.0
blast CAE67165 Hypothetical protein CBG12593 [Caenorhabditis briggsae] 0.0
blast AAB96754 Blistered cuticle protein 4, isoform d [Caenorhabditis elegans] ref|NP_001021543.1| K04F10.4d [Caenorhabditis elegans] pir||D87803 protein bli-4D [imported] - Caenorhabditis elegans 0.0
blast AAB96753 Blistered cuticle protein 4, isoform a [Caenorhabditis elegans] ref|NP_001021540.1| K04F10.4a [Caenorhabditis elegans] 0.0
blast AAO12410 Blistered cuticle protein 4, isoform f [Caenorhabditis elegans] ref|NP_001021545.1| K04F10.4f [Caenorhabditis elegans] 0.0
blast P51559 Endoprotease bli-4 precursor (Blisterase) (Blistered cuticle protein 4) 0.0
blast AAB96757 Blistered cuticle protein 4, isoform c [Caenorhabditis elegans] ref|NP_001021542.1| K04F10.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002884 EMBL-EBI Peptidase S8B, proprotein convertase, P domain 2.9E-70
Pfam IPR000209 EMBL-EBI Peptidase S8 and S53, subtilisin, kexin, sedolisin 2.2E-113
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021543.1 1 873-892
K04F10.4d 1 873-892
Sequence
Target Sequence 
>C. ELEGANS:194220_X_AT
caaagcctcggccaattgtgggtcgtttccaacttaatttcactttagatgtgaatggat
gcgagtcaggaacccctgttttatatttggaacacgttcaagtgcatgctactggtcgat
atctgaaacgtggcgatctgaagcttacgctcttctcaccatccggcacccgatcggttc
ttcttccaccacgaccacaagatttcaatgctaacggattccacaagtggcctttccttt
cagtccaacaatggggagaagatccacgtggaacatggcttctcatggtggaatcagtca
ccactaatccagctgctactggtacgttccacgattggactcttcttctttatggaactg
ctgatccagctcaatcaggtgatcctgtctactcggctactcctgcaacatcccaaggag
ttctctcacgcgttcatcaactcacttctcaggtgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGCCTCGGCCAATTGTGGGTCG 75 189 1531 Antisense
GAATGGATGCGAGTCAGGAACCCCT 212 331 1583 Antisense
GTGCATGCTACTGGTCGATATCTGA 689 479 1632 Antisense
CGATCTGAAGCTTACGCTCTTCTCA 145 249 1664 Antisense
GATTCCACAAGTGGCCTTTCCTTTC 461 425 1747 Antisense
TCAGTCACCACTAATCCAGCTGCTA 480 623 1824 Antisense
CCAGCTGCTACTGGTACGTTCCACG 313 273 1839 Antisense
CACGATTGGACTCTTCTTCTTTATG 602 197 1860 Antisense
ATCAGGTGATCCTGTCTACTCGGCT 459 27 1904 Antisense
GCAACATCCCAAGGAGTTCTCTCAC 581 319 1935 Antisense
GTTCATCAACTCACTTCTCAGGTGT 283 445 1962 Antisense
Sequence Source Affymetrix Proprietary Database