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[194195_x_at] importin alpha, nuclear transport factor

Description CEK117H8R_rc /REP_DB=TREMBL Accession /GB=D73363 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk117h8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 29      4grp_high_8_8 > 03      6grp_highest_nonorm > 10      6grp_total > 28      4grp_total > 07      6grp_highest_sub > De      6grp_cgc > 05      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 734
1 2 3 4 range
A 1546.7
(1.7)
P 		1288.6
(-3)
P 		1352.3
(-1.8)
P 		1617.1
(3)
P 		329
B 1387.1
(0.7)
P 		933.9
(-3)
P 		1184.2
(-1)
P 		1668.2
(3)
P 		734
C 1228.3
(-1.6)
P 		1137.4
(-3)
P 		1431.6
(1.5)
P 		1529.2
(3)
P 		392
avr 1387.4
(0.3) 		1120
(-3) 		1322.7
(-0.5) 		1604.8
(3) 		485

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1105
1 2 3 4 5 6 range
A 1677.3
(3)
P 		1334.4
(0.5)
P 		1084.4
(-1.3)
P 		852.5
(-3)
P 		1114.6
(-1.1)
P 		1364.2
(0.7)
P 		825
B 1827.1
(3)
P 		1391.8
(0.6)
P 		734.5
(-3)
P 		1162.7
(-0.6)
P 		974.2
(-1.7)
P 		1166.4
(-0.6)
P 		1093
C 1839.5
(3)
P 		1201.6
(-0.6)
P 		1040.3
(-1.6)
P 		789.8
(-3)
P 		1232.7
(-0.5)
P 		1000.2
(-1.8)
P 		1050
avr 1781.3
(3) 		1309.3
(-0.3) 		953.1
(-2.9) 		935
(-3) 		1107.2
(-1.8) 		1176.9
(-1.3) 		846
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 333
1 2 3 4 range
A 563.2
(3)
P 		279.7
(-2.2)
P 		353.5
(-0.9)
P 		236.7
(-3)
P 		327
B 442.3
(3)
P 		230.6
(-3)
P 		274.4
(-1.8)
P 		355.5
(0.5)
P 		212
C 406.6
(0.7)
P 		327
(-1.3)
P 		499
(3)
P 		257.8
(-3)
P 		241
avr 470.7
(3) 		279.1
(-3) 		375.6
(0) 		283.3
(-2.9) 		192

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 523
1 2 3 4 5 6 range
A 309.2
(-0.8)
P 		474.6
(3)
P 		236.2
(-2.5)
P 		216.2
(-3)
P 		336.3
(-0.2)
P 		332.1
(-0.3)
P 		258
B 635.7
(3)
P 		463.3
(0.7)
P 		178.4
(-3)
P 		308
(-1.3)
P 		395.8
(-0.1)
P 		434.9
(0.4)
P 		457
C 681.8
(3)
P 		366.6
(-0.6)
P 		264.8
(-1.8)
P 		158.8
(-3)
P 		398.9
(-0.2)
P 		334.8
(-1)
P 		523
avr 542.2
(3) 		434.8
(1) 		226.5
(-3) 		227.7
(-3) 		377
(-0.1) 		367.3
(-0.3) 		316
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194195_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21300
Sequence Type Exemplar sequence
Representative Public ID CEK117H8R NCBI
Target Description CEK117H8R_rc /REP_DB=TREMBL Accession /GB=D73363 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk117h8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068826(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068826 NCBI Caenorhabditis elegans IMportin Alpha family member (ima-3) (ima-3) mRNA, complete cds. 11/11 A 
F32E10.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:7558687:7561648:-1 gene:F32E10.4 11/11 None
F32E10.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:7558687:7561650:-1 gene:F32E10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7558762-7559089(-) 100.0 100.0
Public Domain and Genome References
Gene Title importin alpha, nuclear transport factor
Gene Symbol ima-3
Ensembl F32E10.4
Entrez Gene 177533 Entrez gene
SwissProt Q19969 EMBL-EBI
WormBase CE20745 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1592276_AT similar to Importin alpha-4 subunit (Karyopherin alpha-4 subunit) (Qip1 protein) cfa
CANINE_2:CFA.20684.1.S1_S_AT similar to Importin alpha-4 subunit (Karyopherin alpha-4 subunit) (Qip1 protein) cfa
CANINE_2:CFAAFFX.22101.1.S1_S_AT similar to Importin alpha-4 subunit (Karyopherin alpha-4 subunit) (Qip1 protein) cfa
CANINE_2:CFAAFFX.375.1.S1_AT similar to Importin alpha-4 subunit (Karyopherin alpha-4 subunit) (Qip1 protein) cfa
DROSOPHILA_2:1635566_S_AT karyopherin 3 dm
DROSGENOME1:153795_AT karyopherin 3 dm
CHICKEN:GGA.16047.1.S1_AT karyopherin alpha 4 (importin alpha 3) gga
CHICKEN:GGA.16047.1.S1_S_AT karyopherin alpha 4 (importin alpha 3) gga
CHICKEN:GGAAFFX.11880.1.S1_S_AT karyopherin alpha 4 (importin alpha 3) gga
HG-U133A:209653_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133_PLUS_2:209653_AT karyopherin alpha 4 (importin alpha 3) hs
HG-FOCUS:209653_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133A_2:209653_AT karyopherin alpha 4 (importin alpha 3) hs
HU35KSUBD:RC_Z39821_AT karyopherin alpha 4 (importin alpha 3) hs
HUGENEFL:Y12393_S_AT karyopherin alpha 4 (importin alpha 3) hs
U133_X3P:G1928974_3P_AT karyopherin alpha 4 (importin alpha 3) hs
U133_X3P:HS.288193.0.S2_3P_X_AT karyopherin alpha 4 (importin alpha 3) hs
U133_X3P:HS.288193.0.S2_3P_AT karyopherin alpha 4 (importin alpha 3) hs
U133_X3P:HS.288193.0.S1_3P_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133_PLUS_2:225267_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133B:225267_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133_PLUS_2:225268_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U133B:225268_AT karyopherin alpha 4 (importin alpha 3) hs
HG-U95C:48814_AT karyopherin alpha 4 (importin alpha 3) hs
MG-U74AV2:100320_AT karyopherin (importin) alpha 4 mm
MG-U74BV2:107588_AT karyopherin (importin) alpha 4 mm
MG-U74BV2:115627_AT karyopherin (importin) alpha 4 mm
MG-U74CV2:170101_AT karyopherin (importin) alpha 4 mm
MOE430A:1417974_AT karyopherin (importin) alpha 4 mm
MOUSE430A_2:1417974_AT karyopherin (importin) alpha 4 mm
MOUSE430_2:1417974_AT karyopherin (importin) alpha 4 mm
MOE430A:1417975_AT karyopherin (importin) alpha 4 mm
MOUSE430A_2:1417975_AT karyopherin (importin) alpha 4 mm
MOUSE430_2:1417975_AT karyopherin (importin) alpha 4 mm
MOE430B:1442928_AT Karyopherin (importin) alpha 4, mRNA (cDNA clone MGC:19244 IMAGE:3672708) mm
MOUSE430_2:1442928_AT Karyopherin (importin) alpha 4, mRNA (cDNA clone MGC:19244 IMAGE:3672708) mm
MU19KSUBB:TC32326_AT Karyopherin (importin) alpha 4, mRNA (cDNA clone MGC:19244 IMAGE:3672708) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6606 protein import into nucleus inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8565 protein transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83354 Importin alpha family protein 3 [Caenorhabditis elegans] ref|NP_501227.1| IMportin Alpha family member (ima-3) [Caenorhabditis elegans] gb|AAB97171.1| importin alpha 3 [Caenorhabditis elegans] sp|Q19969|IMA3_CAEEL Importin alpha-3 subunit (Karyopherin alpha-3 subunit) 0.0
blast G88733 protein F32E10.4 [imported] - Caenorhabditis elegans 0.0
blast CAE61916 Hypothetical protein CBG05912 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000225 EMBL-EBI Armadillo 4.4E-15
Pfam IPR000225 EMBL-EBI Armadillo 5.6E-14
Pfam IPR000225 EMBL-EBI Armadillo 3.0E-7
Pfam IPR000225 EMBL-EBI Armadillo 9.8E-11
Pfam IPR000225 EMBL-EBI Armadillo 5.5E-7
Pfam IPR000225 EMBL-EBI Armadillo 3.3E-11
Pfam IPR002652 EMBL-EBI Importin alpha-like protein, beta-binding region 3.5E-34
Sequence
Target Sequence 
>C. ELEGANS:194195_X_AT
cccctagtttgctcatttcctagtcatccatcatcattctcgatttcattagttctcgga
gaatctcgttgataggccaaaaattccaatcaccctgctcctcttagttctcgtggactc
cagtattctttttcttgaaaccccacccatcccgtaaggcctttttaaaggtatatcgag
ccgtccctcttctcatcatccatcttcttttctcattttccctctcatcttcctcatccc
attttcctttctcccgttttgctattctcgattgtctcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCCTAGTTTGCTCATTTCCTAGTC 386 201 29 Antisense
CTAGTCATCCATCATCATTCTCGAT 288 225 48 Antisense
ATCATTCTCGATTTCATTAGTTCTC 189 31 61 Antisense
GATTTCATTAGTTCTCGGAGAATCT 264 427 70 Antisense
GGAGAATCTCGTTGATAGGCCAAAA 460 519 86 Antisense
AGGCCAAAAATTCCAATCACCCTGC 492 53 102 Antisense
TCTTAGTTCTCGTGGACTCCAGTAT 571 613 130 Antisense
TGGACTCCAGTATTCTTTTTCTTGA 39 549 142 Antisense
TAAAGGTATATCGAGCCGTCCCTCT 258 641 194 Antisense
TCCCGTTTTGCTATTCTCGATTGTC 565 597 280 Antisense
GTTTTGCTATTCTCGATTGTCTCGA 116 449 284 Antisense
Sequence Source Affymetrix Proprietary Database