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[194188_x_at]

Description CEC4543_rc /REP_DB=TREMBL Accession /GB=C10454 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk146c3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 31      6grp_total > 17      4grp_total > 14      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1160
1 2 3 4 range
A 1361
(2.8)
P 		753.5
(-1.6)
P 		555
(-3)
P 		1382.2
(3)
P 		827
B 1316.7
(0.7)
P 		771.9
(-2.5)
P 		687.8
(-3)
P 		1714.7
(3)
P 		1027
C 1348
(2.4)
P 		717
(-2.6)
P 		673.3
(-3)
P 		1422.2
(3)
P 		749
avr 1341.9
(1.9) 		747.5
(-2.2) 		638.7
(-3) 		1506.4
(3) 		868

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 852
1 2 3 4 5 6 range
A 1155.3
(3)
P 		1015.3
(0.6)
P 		1117.3
(2.3)
P 		922.8
(-1.1)
P 		811.7
(-3)
P 		901.1
(-1.4)
P 		344
B 1266.2
(3)
P 		1047.9
(0.1)
P 		885.1
(-2.1)
P 		816.4
(-3)
P 		957.5
(-1.1)
P 		1055.4
(0.2)
P 		450
C 1432.6
(3)
P 		1102
(0.7)
P 		818.7
(-1.3)
P 		581
(-3)
P 		951.8
(-0.4)
P 		859.5
(-1)
P 		852
avr 1284.7
(3) 		1055.1
(0.3) 		940.4
(-1) 		773.4
(-3) 		907
(-1.4) 		938.7
(-1.1) 		511
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 350
1 2 3 4 range
A 495.5
(3)
P 		163.6
(-2.7)
P 		145.1
(-3)
P 		202.3
(-2)
P 		350
B 419.9
(3)
P 		190.6
(-2.3)
P 		159.4
(-3)
P 		365.4
(1.7)
P 		261
C 446.2
(3)
P 		206.1
(-3)
P 		234.7
(-2.3)
P 		239.8
(-2.2)
P 		240
avr 453.9
(3) 		186.8
(-2.8) 		179.7
(-3) 		269.2
(-1) 		274

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 414
1 2 3 4 5 6 range
A 213
(-3)
P 		361.1
(3)
P 		243.3
(-1.8)
P 		234
(-2.1)
P 		244.9
(-1.7)
P 		219.4
(-2.7)
P 		148
B 440.6
(3)
P 		348.8
(0.6)
P 		214.9
(-3)
P 		216.2
(-3)
P 		389
(1.6)
P 		393.6
(1.8)
P 		226
C 531
(3)
P 		336.3
(0.2)
P 		208.4
(-1.7)
P 		116.8
(-3)
P 		308
(-0.2)
P 		287.7
(-0.5)
P 		414
avr 394.9
(3) 		348.7
(1.7) 		222.2
(-2) 		189
(-3) 		314
(0.6) 		300.2
(0.2) 		206
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194188_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20806
Sequence Type Exemplar sequence
Representative Public ID CEC4543 NCBI
Target Description CEC4543_rc /REP_DB=TREMBL Accession /GB=C10454 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk146c3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061303(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061303 NCBI Caenorhabditis elegans K01A2.8a (K01A2.8) mRNA, complete cds. 11/11 None
K01A2.8a ENSEMBL cdna:known chromosome:CEL140:II:309554:311349:-1 gene:K01A2.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173419 2 1 NM_061303 194188_x_at (11), 194226_x_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:310932-311214(-) 100.0 100.0
Public Domain and Genome References
Ensembl K01A2.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69501 Mirp k channel accessory subunit protein 2, isoform a [Caenorhabditis elegans] ref|NP_493704.1| K01A2.8a [Caenorhabditis elegans] 4.0E-66
blast AAQ96604 MiRP4 [Caenorhabditis elegans] 6.0E-65
blast AAZ94292 Mirp k channel accessory subunit protein 2, isoform d [Caenorhabditis elegans] gb|AAQ96603.1| MPS-3 [Caenorhabditis elegans] 2.0E-51
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493704.1 4 29-51,66-88,101-123,133-155
K01A2.8a 4 29-51,66-88,101-123,133-155
Sequence
Target Sequence 
>C. ELEGANS:194188_X_AT
gcactaaaacactagcgctttgaggacaaagcacttttgagagggtaggatggcgtgccg
ttaccgtcatctgttatttttctttttgggaatctgagttctgtaagcctaacatcaaaa
gcttcgggacgaagctgctcaaaaagggcggcatgtgagaggtcagttctatactgcttc
tcggtttctatttatcgagtgcccttgatcattgccatctgtcaacgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACTAAAACACTAGCGCTTTGAGG 538 313 29 Antisense
GAGGGTAGGATGGCGTGCCGTTACC 611 379 69 Antisense
GTTACCGTCATCTGTTATTTTTCTT 59 447 88 Antisense
GAATCTGAGTTCTGTAAGCCTAACA 641 325 118 Antisense
GCCTAACATCAAAAGCTTCGGGACG 167 283 135 Antisense
GACGAAGCTGCTCAAAAAGGGCGGC 206 379 156 Antisense
AAAGGGCGGCATGTGAGAGGTCAGT 115 113 171 Antisense
GAGGTCAGTTCTATACTGCTTCTCG 113 403 187 Antisense
TACTGCTTCTCGGTTTCTATTTATC 349 647 200 Antisense
TTATCGAGTGCCCTTGATCATTGCC 165 679 220 Antisense
TGATCATTGCCATCTGTCAACGACA 204 565 234 Antisense
Sequence Source Affymetrix Proprietary Database