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[194180_x_at]

Description AV190462 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=B0273.4A /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV190462 /WB_GENE_ID=B0273.4A /WP=CE16790 /CHR=4 /FEA=Genomic Cluster /GEN=unc-5 /DEF=axon guidance protein; Caenorhabditis elegans cDNA clone:yk571c9 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 24      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 61      6grp_total > 12      4grp_total > 53      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 411.5
(3)
P 		278.3
(-0.4)
P 		247.2
(-1.2)
P 		175.7
(-3)
P 		236
B 303.7
(1)
P 		343.8
(3)
P 		266.8
(-0.9)
P 		224.2
(-3)
P 		120
C 368.9
(3)
P 		321.9
(1)
P 		282.9
(-0.7)
P 		229.4
(-3)
P 		140
avr 361.4
(3) 		314.7
(1.2) 		265.6
(-0.8) 		209.8
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 313.3
(-1.6)
P 		460.2
(3)
P 		330.4
(-1.1)
P 		268.8
(-3)
P 		321.7
(-1.3)
P 		310.4
(-1.7)
P 		191
B 473
(3)
P 		455.7
(2.4)
P 		419
(1)
P 		325.5
(-2.4)
P 		308.3
(-3)
P 		332.3
(-2.1)
P 		165
C 456.6
(3)
P 		450.1
(2.8)
P 		365.9
(-0.3)
P 		443.7
(2.5)
P 		356.5
(-0.6)
P 		289.9
(-3)
P 		167
avr 414.3
(1.3) 		455.3
(3) 		371.8
(-0.5) 		346
(-1.5) 		328.8
(-2.3) 		310.9
(-3) 		144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 149.8
(3)
P 		60.4
(-1.3)
P 		64.6
(-1.1)
P 		25.7
(-3)
P 		124
B 96.9
(3)
P 		84.9
(1.5)
P 		61.8
(-1.3)
P 		47.8
(-3)
P 		49
C 122.1
(3)
P 		92.5
(0.9)
P 		98.6
(1.3)
P 		38.7
(-3)
P 		83
avr 122.9
(3) 		79.3
(-0.1) 		75
(-0.4) 		37.4
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 57.8
(-3)
P 		163.7
(3)
P 		72
(-2.2)
P 		68.2
(-2.4)
P 		97.1
(-0.8)
P 		75.6
(-2)
P 		106
B 164.6
(3)
P 		151.7
(2)
P 		101.7
(-1.8)
P 		86.2
(-3)
P 		125.3
(-0)
P 		123.9
(-0.1)
P 		78
C 169.2
(3)
P 		137.3
(0.6)
P 		93.1
(-2.7)
P 		89.2
(-3)
P 		115.4
(-1)
P 		97
(-2.4)
P 		80
avr 130.5
(1.2) 		150.9
(3) 		88.9
(-2.3) 		81.2
(-3) 		112.6
(-0.3) 		98.8
(-1.5) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194180_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24992
Sequence Type Exemplar sequence
Representative Public ID AV190462 NCBI
Target Description AV190462 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=B0273.4A /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV190462 /WB_GENE_ID=B0273.4A /WP=CE16790 /CHR=4 /FEA=Genomic Cluster /GEN=unc-5 /DEF=axon guidance protein; Caenorhabditis elegans cDNA clone:yk571c9 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068422(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068422 NCBI Caenorhabditis elegans UNCoordinated family member (unc-5) (unc-5) mRNA, complete cds. 11/11 A 
B0273.4a ENSEMBL cdna:known chromosome:CEL140:IV:5495666:5516250:-1 gene:B0273.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012464 7/11 Cross Hyb Matching Probes None
GENEFINDER00000012471 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177334 2 1 NM_068422 192494_s_at (11), 194180_x_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5495738-5516257(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0273.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB88355 Uncoordinated protein 5, isoform a [Caenorhabditis elegans] ref|NP_500823.1| UNCoordinated family member (unc-5) [Caenorhabditis elegans] sp|Q26261|UNC5_CAEEL Netrin receptor unc-5 precursor (Uncoordinated protein 5) 0.0
blast AAB23867 UNC-5 [Caenorhabditis elegans] 0.0
blast AAB23866 UNC-5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000906 EMBL-EBI ZU5 domain 1.3E-58
Pfam IPR000488 EMBL-EBI Death domain 6.5E-22
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 5.5E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500823.1 1 338-360
B0273.4a 1 338-360
Sequence
Target Sequence 
>C. ELEGANS:194180_X_AT
gagccggagctctatgatgcaacaagtgaagaaagagaaaagggatccgtttgtgtagag
ttccgattgccatttggagtgaaggatgaactggcaaggcttttggatatgccaaatgaa
tcacattctgattggagaggattggcaaagaaattgcattacgatagatatttgcagttc
ttcgcctcattccctgattgctctcccacatcacttctcctggatctttgggaagcttca
tcttctggatcagctcgtgcagttccagatcttcttcaaactcttcgtgtaatgggacga
cccgatgcagtaatggtcctggaacgatttctatcagcttttccacaaattgtgtcccca
taagctcaattttttgcacaaacacaactagattctacgtcgtttttctatgggtattct
gttctgcgaacaatcacattttttcttcattttttgctctttttgtcaatgaaaccggta
atatatgcctgcccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCGGAGCTCTATGATGCAACAA 640 385 2411 Antisense
TAGATATTTGCAGTTCTTCGCCTCA 64 653 2575 Antisense
CATCACTTCTCCTGGATCTTTGGGA 176 213 2619 Antisense
GAAGCTTCATCTTCTGGATCAGCTC 355 341 2642 Antisense
GATCAGCTCGTGCAGTTCCAGATCT 372 419 2658 Antisense
GATCTTCTTCAAACTCTTCGTGTAA 609 419 2678 Antisense
GAACGATTTCTATCAGCTTTTCCAC 484 347 2732 Antisense
GTGTCCCCATAAGCTCAATTTTTTG 20 485 2762 Antisense
GATTCTACGTCGTTTTTCTATGGGT 556 427 2801 Antisense
TTGCTCTTTTTGTCAATGAAACCGG 131 701 2864 Antisense
GAAACCGGTAATATATGCCTGCCCC 7 359 2881 Antisense
Sequence Source Affymetrix Proprietary Database