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[194175_x_at]

Description CEK131HXR_rc /REP_DB=TREMBL Accession /GB=D65143 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk131h10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 10      6grp_total > 05      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 42      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1431
1 2 3 4 range
A 2256.2
(3)
P 		1102.4
(-2.5)
P 		1007.7
(-3)
P 		1372
(-1.2)
P 		1249
B 2292.4
(3)
P 		1059.2
(-2.2)
P 		861.8
(-3)
P 		1369.1
(-0.9)
P 		1431
C 1974.3
(3)
P 		1010.3
(-2.9)
P 		989.6
(-3)
P 		1549.9
(0.4)
P 		985
avr 2174.3
(3) 		1057.3
(-2.5) 		953
(-3) 		1430.3
(-0.7) 		1221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1451
1 2 3 4 5 6 range
A 2020.3
(3)
P 		1846.1
(2)
P 		1429.2
(-0.2)
P 		1046
(-2.3)
P 		1519.9
(0.3)
P 		921.2
(-3)
P 		1099
B 1730.1
(1.9)
P 		1993.7
(3)
P 		615.5
(-3)
P 		1371.7
(0.3)
P 		994.4
(-1.4)
P 		1107.4
(-0.9)
P 		1378
C 1735.4
(3)
P 		1734.2
(3)
P 		1197.5
(0.2)
P 		938.2
(-1.1)
P 		1167.2
(0.1)
P 		569.3
(-3)
P 		1166
avr 1828.6
(2.8) 		1858
(3) 		1080.7
(-1.7) 		1118.6
(-1.5) 		1227.2
(-0.8) 		866
(-3) 		992
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 622
1 2 3 4 range
A 821.5
(3)
P 		239.3
(-2.6)
P 		263.4
(-2.4)
P 		200.8
(-3)
P 		621
B 731
(3)
P 		261.6
(-2.3)
P 		199.7
(-3)
P 		291.8
(-2)
P 		531
C 653.6
(3)
P 		290.5
(-2.6)
P 		344.9
(-1.7)
P 		261.3
(-3)
P 		392
avr 735.4
(3) 		263.8
(-2.8) 		269.3
(-2.8) 		251.3
(-3) 		484

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 514
1 2 3 4 5 6 range
A 372.4
(-0.9)
P 		656.6
(3)
P 		311.2
(-1.8)
P 		265.3
(-2.4)
P 		458.6
(0.3)
P 		224.3
(-3)
P 		432
B 602
(2.3)
P 		663.6
(3)
P 		149.5
(-3)
P 		363.3
(-0.5)
P 		404
(-0)
P 		413
(0.1)
P 		514
C 643.2
(3)
P 		529.1
(1.5)
P 		304.8
(-1.5)
P 		188.7
(-3)
P 		377.7
(-0.5)
P 		190.6
(-3)
P 		455
avr 539.2
(1.7) 		616.4
(3) 		255.2
(-3) 		272.4
(-2.7) 		413.4
(-0.4) 		276
(-2.7) 		361
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194175_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20722
Sequence Type Exemplar sequence
Representative Public ID CEK131HXR NCBI
Target Description CEK131HXR_rc /REP_DB=TREMBL Accession /GB=D65143 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk131h10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063199(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063199 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-4) (pde-4) mRNA, complete cds. 11/11 None
R153.1b ENSEMBL cdna:known chromosome:CEL140:II:7627174:7649877:-1 gene:R153.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174235 6 1 NM_063199 192893_at (11), 194175_x_at (11)
NM_063200 192893_at (11)
NM_182144 192893_at (11)
NM_182145 192893_at (11)
NM_182146 192893_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7627180-7627520(-) 100.0 100.0
Public Domain and Genome References
Ensembl R153.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK77625 Hypothetical protein R153.1b [Caenorhabditis elegans] ref|NP_495600.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] 0.0
blast AAO38608 Hypothetical protein R153.1e [Caenorhabditis elegans] ref|NP_871946.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] 0.0
blast AAO38607 Hypothetical protein R153.1d [Caenorhabditis elegans] ref|NP_871945.1| PhosphoDiEsterase family member (pde-4) [Caenorhabditis elegans] sp|Q22000|PDE4_CAEEL Probable 3',5'-cyclic phosphodiesterase pde-4 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:194175_X_AT
ttttctttgcttttgtctttctatcgggtttttcatgtttttttcctaaaattctactcg
cactttttccggtgctcgttttcttctgtctcttactttttgtttcaaaattaataatta
gatccaatagattaagcttctcctttttctatcaaaaatctttattcttctagttccttt
cctccttccaatgttcaaacttccagcttctatatgtatgtacacattgcatctttcacg
ctttaaatcagatttctatacagctctatggattccgtgtcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTCTTTGCTTTTGTCTTTCTATC 376 677 37 Antisense
GTCTTTCTATCGGGTTTTTCATGTT 60 469 51 Antisense
TAAAATTCTACTCGCACTTTTTCCG 442 639 83 Antisense
TTCTTCTGTCTCTTACTTTTTGTTT 138 691 117 Antisense
GATTAAGCTTCTCCTTTTTCTATCA 16 431 166 Antisense
TCAAAAATCTTTATTCTTCTAGTTC 503 627 188 Antisense
TTCCTCCTTCCAATGTTCAAACTTC 155 699 215 Antisense
GTTCAAACTTCCAGCTTCTATATGT 245 445 229 Antisense
TACACATTGCATCTTTCACGCTTTA 521 641 257 Antisense
AAATCAGATTTCTATACAGCTCTAT 527 117 281 Antisense
ACAGCTCTATGGATTCCGTGTCGAA 318 107 296 Antisense
Sequence Source Affymetrix Proprietary Database