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[194141_at]

Description ZC101.2E /REP_DB=WormBase Gene ID /WP=CE18424 /GEN=unc-52 /TR=Q9XTI5 /GB=CAB07708.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=IG (immunoglobulin) superfamily (17 domains), Low-density lipoprotein receptor domain class A (3 domains), Laminin EGF-like (Domains III and V) (5 domains), Laminin B (Domain IV) (2 domains), Laminin G domain
Cluster 6grp_high1_10_10 > 14      6grp_total > 03      4grp_total > 52      6grp_cgc > 51      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 132.6
(1.7)
P 		159.8
(3)
A 		100.8
(0.1)
A 		35.7
(-3)
A 		124
B 169.1
(0.8)
P 		265
(3)
M 		186.8
(1.2)
P 		4.6
(-3)
A 		260
C 159.2
(0.8)
P 		230.5
(3)
P 		141.3
(0.2)
P 		39.9
(-3)
A 		191
avr 153.6
(1) 		218.4
(3) 		143
(0.6) 		26.7
(-3) 		192

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 259.9
(1.9)
P 		297.7
(3)
P 		109.1
(-2.6)
A 		270.9
(2.2)
P 		142.7
(-1.6)
P 		96.8
(-3)
P 		201
B 181.5
(1.9)
P 		217.9
(3)
P 		81.6
(-1.2)
P 		177.4
(1.8)
P 		112.4
(-0.2)
M 		21
(-3)
A 		197
C 266.5
(3)
P 		175.2
(0.1)
P 		215.3
(1.4)
P 		168.8
(-0.1)
P 		228.3
(1.8)
P 		76.8
(-3)
P 		190
avr 236
(3) 		230.3
(2.8) 		135.3
(-0.5) 		205.7
(1.9) 		161.1
(0.4) 		64.9
(-3) 		171
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 48.3
(3)
P 		34.7
(1.1)
A 		26.4
(-0)
A 		5.2
(-3)
A 		43
B 53.9
(1.9)
P 		65.4
(3)
M 		43.3
(0.9)
P 		1
(-3)
A 		64
C 52.7
(1.6)
P 		66.3
(3)
P 		49.3
(1.3)
P 		6.7
(-3)
A 		60
avr 51.6
(2.5) 		55.5
(3) 		39.7
(1.1) 		4.3
(-3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 47.9
(-1.2)
P 		105.9
(3)
P 		23.8
(-3)
A 		68.7
(0.3)
P 		43.1
(-1.6)
P 		23.6
(-3)
P 		82
B 63.1
(2.1)
P 		72.5
(3)
P 		19.8
(-1.9)
P 		47
(0.6)
P 		45.7
(0.5)
M 		7.8
(-3)
A 		65
C 98.8
(3)
P 		53.5
(-0.7)
P 		54.8
(-0.6)
P 		33.9
(-2.3)
P 		73.9
(1)
P 		25.7
(-3)
P 		73
avr 69.9
(2.2) 		77.3
(3) 		32.8
(-1.6) 		49.9
(0.2) 		54.2
(0.6) 		19
(-3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194141_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3583
Sequence Type Exemplar sequence
Representative Public ID ZC101.2E NCBI
Target Description ZC101.2E /REP_DB=WormBase Gene ID /WP=CE18424 /GEN=unc-52 /TR=Q9XTI5 /GB=CAB07708.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=IG (immunoglobulin) superfamily (17 domains), Low-density lipoprotein receptor domain class A (3 domains), Laminin EGF-like (Domains III and V) (5 domains), Laminin B (Domain IV) (2 domains), Laminin G domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064643(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064643 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 None
SNAP00000016883 ENSEMBL cdna:SNAP chromosome:CEL140:II:14647370:14649683:-1 11/11 None
GENEFINDER00000016886 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14646309:14653706:-1 11/11 None
ZC101.2e ENSEMBL cdna:known chromosome:CEL140:II:14647215:14666743:-1 gene:ZC101.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175126 5 1 NM_001027317 172932_x_at (11)
NM_064643 172932_x_at (10), 194141_at (11)
NM_064644 172932_x_at (11)
NM_064645 172932_x_at (11)
NM_064646 174785_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14647366-14666740(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC101.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07708 Hypothetical protein ZC101.2e [Caenorhabditis elegans] emb|CAB07569.1| Hypothetical protein ZC101.2e [Caenorhabditis elegans] ref|NP_497044.3| UNCoordinated family member (unc-52) [Caenorhabditis elegans] sp|Q06561|UNC52_CAEEL Basement membrane proteoglycan precursor (Perlecan homolog) (Uncoordinated protein 52) pir||T19821 hypothetical protein ZC101.2e - Caenorhabditis elegans 0.0
blast CAB07706 Hypothetical protein ZC101.2a [Caenorhabditis elegans] emb|CAB07567.1| Hypothetical protein ZC101.2a [Caenorhabditis elegans] ref|NP_497046.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] 0.0
blast AAA28156 basement membrane proteoglycan 0.0
blast AAD25092 UNC-52/Perlecan [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001791 EMBL-EBI Laminin G 5.0E-5
Pfam IPR001791 EMBL-EBI Laminin G 5.3E-33
Pfam IPR006209 EMBL-EBI EGF-like domain 1.2E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 7.6E-4
Pfam IPR001791 EMBL-EBI Laminin G 5.0E-5
Pfam IPR001791 EMBL-EBI Laminin G 5.3E-33
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.6E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 1.2E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 7.6E-4
Pfam IPR001791 EMBL-EBI Laminin G 5.0E-5
Pfam IPR001791 EMBL-EBI Laminin G 5.3E-33
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-18
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.1E-20
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 8.1E-20
Pfam IPR000034 EMBL-EBI Laminin B 9.3E-79
Pfam IPR000034 EMBL-EBI Laminin B 5.3E-73
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 7.8E-20
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.7E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0023
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.6E-8
Sequence
Target Sequence 
>C. ELEGANS:194141_AT
tatgatggcccaacctgtagccttttcaagccaatcgaacacgcagctcgatttgacgga
gacgcatttatcgagctttcgtcagatgagttcccacatttgacatctgaaaaggatgaa
attgtggcgttcaagttcaaaaccgagcaacaaaatggtgtgctgttgtggcaaggacaa
cgtcctaccgttcaacaaatggaggactatatatcagttggaattgtcaatgggcatctg
cacttttcctacgaacttggtggtggagctgcgcatcttatctccgaagaaagagtagat
gatggaaaagagcacagtgtgagatttgaaagaaagggaagagaagggcaaatgcgaatt
gacaactaccgtgaagtcgacggtcggagcacgggaatccttgcgatgctcaatgttgac
gggaatattttcgttggcggggtgcctgatatctccaaagccaccggtggcttgttctcc
aacaactttgtcggatgcatcgctgacgtagaattgaatggggtgaagttggacttgatg
gccactgcgatcgatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGATGGCCCAACCTGTAGCCTTT 102 657 9514 Antisense
CGAACACGCAGCTCGATTTGACGGA 521 245 9549 Antisense
CGGAGACGCATTTATCGAGCTTTCG 550 243 9570 Antisense
GAGCTTTCGTCAGATGAGTTCCCAC 517 387 9586 Antisense
TGTCAATGGGCATCTGCACTTTTCC 173 559 9738 Antisense
TGGTGGAGCTGCGCATCTTATCTCC 499 553 9774 Antisense
TCGGAGCACGGGAATCCTTGCGATG 706 605 9897 Antisense
GAATATTTTCGTTGGCGGGGTGCCT 639 323 9936 Antisense
GGTGCCTGATATCTCCAAAGCCACC 152 501 9954 Antisense
ACTTTGTCGGATGCATCGCTGACGT 276 103 9998 Antisense
ACTTGATGGCCACTGCGATCGATGG 248 93 10046 Antisense
Sequence Source Affymetrix Proprietary Database