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[194140_at] Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain

Description Y75B12B.6 /REP_DB=WormBase Gene ID /WP=CE20375 /TR=Q9XWB7 /GB=CAA21765.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 93      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 81      6grp_total > 80      4grp_total > 55      6grp_highest_sub > 04      6grp_cgc > 77      6grp_high1_sub > 04      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 384.8
(3)
P 		269.3
(-1.5)
P 		230.2
(-3)
P 		284
(-0.9)
P 		155
B 288.1
(3)
P 		281.9
(-3)
P 		282.3
(-2.6)
P 		284.2
(-0.8)
P 		6
C 387
(3)
P 		267.3
(-1.4)
P 		325.3
(0.8)
P 		222.4
(-3)
P 		165
avr 353.3
(3) 		272.8
(-2.4) 		279.3
(-1.9) 		263.5
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 185.3
(-3)
P 		311.2
(0.3)
P 		184.7
(-3)
P 		414.6
(3)
P 		290.4
(-0.2)
P 		395
(2.5)
P 		230
B 270.9
(0.5)
P 		202.3
(-3)
P 		290.5
(1.5)
P 		319.8
(3)
P 		217
(-2.2)
P 		270.4
(0.5)
P 		118
C 272.2
(-1.8)
P 		246.2
(-2.6)
P 		233.6
(-3)
P 		421.2
(3)
P 		345.8
(0.6)
P 		268.6
(-1.9)
P 		188
avr 242.8
(-2.7) 		253.2
(-2.3) 		236.3
(-3) 		385.2
(3) 		284.4
(-1.1) 		311.3
(0) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 140.1
(3)
P 		58.5
(-2)
P 		60.2
(-1.9)
P 		41.6
(-3)
P 		99
B 91.9
(3)
P 		69.6
(-1.3)
P 		65.4
(-2.1)
P 		60.6
(-3)
P 		31
C 128.1
(3)
P 		76.9
(-0.4)
P 		113.4
(2)
P 		37.5
(-3)
P 		91
avr 120
(3) 		68.3
(-1.2) 		79.7
(-0.3) 		46.6
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 34.2
(-3)
P 		110.7
(3)
P 		40.2
(-2.5)
P 		105.1
(2.6)
P 		87.6
(1.2)
P 		96.2
(1.9)
P 		77
B 94.3
(1.8)
P 		67.4
(-3)
P 		70.5
(-2.4)
P 		84.7
(0.1)
P 		88.2
(0.7)
P 		100.8
(3)
P 		33
C 100.9
(1.7)
P 		75.1
(-1.2)
P 		59.5
(-3)
P 		84.7
(-0.1)
P 		111.9
(3)
P 		89.9
(0.5)
P 		52
avr 76.5
(0) 		84.4
(1.2) 		56.7
(-3) 		91.5
(2.3) 		95.9
(3) 		95.6
(3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194140_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14036
Sequence Type Exemplar sequence
Representative Public ID Y75B12B.6 NCBI
Target Description Y75B12B.6 /REP_DB=WormBase Gene ID /WP=CE20375 /TR=Q9XWB7 /GB=CAA21765.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074351(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074351 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-2) (plc-2) mRNA, complete cds. 11/11 A 
SNAP00000027061 ENSEMBL cdna:SNAP chromosome:CEL140:V:15197607:15200156:1 11/11 None
GENEFINDER00000027065 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15195309:15203807:1 11/11 None
Y75B12B.6 ENSEMBL cdna:known chromosome:CEL140:V:15195254:15200247:1 gene:Y75B12B.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15195308-15200156(+) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Gene Symbol plc-2
Ensembl Y75B12B.6
Entrez Gene 180029 Entrez gene
SwissProt Q9XWB7 EMBL-EBI
WormBase CE20375 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4435 phosphoinositide phospholipase C activity inferred from electronic annotation QuickGO AmiGO
4629 phospholipase C activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21765 Hypothetical protein Y75B12B.6 [Caenorhabditis elegans] ref|NP_506752.1| PhosphoLipase C family member (plc-2) [Caenorhabditis elegans] 0.0
blast CAE71603 Hypothetical protein CBG18562 [Caenorhabditis briggsae] 0.0
blast CAA21766 Hypothetical protein Y75B12B.7 [Caenorhabditis elegans] ref|NP_506753.2| Serpentine Receptor, class W family member (srw-1) [Caenorhabditis elegans] 2.0E-90
blast CAG00327 unnamed protein product [Tetraodon nigroviridis] 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 5.9E-55
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 1.8E-28
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 5.9E-55
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 1.8E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027065 2 1027-1049,1070-1092
Sequence
Target Sequence 
>C. ELEGANS:194140_AT
acgaagttcagtttccattcgcctctttgacgtacctgatcagatatccaaagatgatgc
cgcatcgccatcagtatccacggctgcaaaattcgaatccagctgccgacggcgtactcg
agaggaggcttcgtttgtcactaactatgagcgtcatgtatacagtttcgacaaaatcat
gttgaatgacatgtcattcattcaattcaatgttcataatgagtcgggtagtgtatttgc
tcaacgaatacttcatattgatactatgaataatggatatcgctttgtgacacttcgtac
tccatctaatcagtgtgctgggccagcatgccttttagttagatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAAGTTCAGTTTCCATTCGCCTC 124 93 2085 Antisense
AGATGATGCCGCATCGCCATCAGTA 515 67 2136 Antisense
CCATCAGTATCCACGGCTGCAAAAT 322 271 2152 Antisense
GCAAAATTCGAATCCAGCTGCCGAC 666 317 2170 Antisense
GACGGCGTACTCGAGAGGAGGCTTC 249 375 2192 Antisense
GAGGCTTCGTTTGTCACTAACTATG 40 405 2209 Antisense
AGTCGGGTAGTGTATTTGCTCAACG 414 61 2306 Antisense
AATGGATATCGCTTTGTGACACTTC 345 163 2356 Antisense
TGACACTTCGTACTCCATCTAATCA 481 571 2372 Antisense
ATCTAATCAGTGTGCTGGGCCAGCA 207 33 2388 Antisense
GGCCAGCATGCCTTTTAGTTAGATT 570 541 2405 Antisense
Sequence Source Affymetrix Proprietary Database