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[194126_s_at]

Description C53B4.4B /REP_DB=WormBase Gene ID /WP=CE20600 /TR=Q18798 /GB=CAA92456.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=PDZ domain (Also known as DHR or GLGF). ; Phorbol esters-diacylglycerol binding domain (C1 domain)
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 60      6grp_total > 36      4grp_total > 38      6grp_highest_sub > Cu      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 474.8
(3)
P 		411.6
(0.3)
P 		349.9
(-2.3)
P 		332.7
(-3)
P 		142
B 490.2
(3)
P 		339
(-1.7)
P 		296.8
(-3)
P 		424.1
(0.9)
P 		193
C 499.8
(3)
P 		387
(-0.7)
P 		317.3
(-3)
P 		388.7
(-0.7)
P 		183
avr 488.3
(3) 		379.2
(-0.9) 		321.3
(-3) 		381.8
(-0.8) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 348
1 2 3 4 5 6 range
A 435.6
(0.6)
P 		573.1
(3)
P 		224.7
(-3)
P 		307.3
(-1.6)
P 		255.6
(-2.5)
P 		418.4
(0.3)
P 		348
B 480
(3)
P 		432.2
(1.8)
P 		340.5
(-0.4)
P 		287.2
(-1.7)
P 		234.5
(-3)
P 		390.8
(0.8)
P 		246
C 546.5
(3)
P 		403
(-0.9)
P 		328.3
(-2.9)
P 		323.5
(-3)
P 		335.3
(-2.7)
P 		326
(-2.9)
P 		223
avr 487.4
(3) 		469.4
(2.5) 		297.8
(-2.4) 		306
(-2.1) 		275.1
(-3) 		378.4
(-0.1) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 172.9
(3)
P 		89.3
(-1)
P 		91.5
(-0.9)
P 		48.7
(-3)
P 		124
B 156.3
(3)
P 		83.7
(-2)
P 		68.8
(-3)
P 		90.4
(-1.5)
P 		88
C 165.5
(3)
P 		111.3
(-0.3)
P 		110.6
(-0.3)
P 		65.5
(-3)
P 		100
avr 164.9
(3) 		94.8
(-1.3) 		90.3
(-1.6) 		68.2
(-3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 80.3
(-1.8)
P 		203.8
(3)
P 		48.9
(-3)
P 		77.9
(-1.9)
P 		77.1
(-1.9)
P 		101.9
(-0.9)
P 		155
B 167
(3)
P 		143.9
(1.5)
P 		82.7
(-2.6)
P 		76.1
(-3)
P 		95.3
(-1.7)
P 		145.7
(1.6)
P 		91
C 202.6
(3)
P 		123
(-0.5)
P 		83.6
(-2.2)
P 		65.1
(-3)
P 		108.5
(-1.1)
P 		109.1
(-1.1)
P 		138
avr 150
(2.5) 		156.9
(3) 		71.7
(-3) 		73
(-2.9) 		93.6
(-1.5) 		118.9
(0.3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194126_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10001
Sequence Type Exemplar sequence
Representative Public ID C53B4.4B NCBI
Target Description C53B4.4B /REP_DB=WormBase Gene ID /WP=CE20600 /TR=Q18798 /GB=CAA92456.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=PDZ domain (Also known as DHR or GLGF). ; Phorbol esters-diacylglycerol binding domain (C1 domain)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069157(11), NM_069158(11), NM_069159(11), NM_001027922(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069157 NCBI Caenorhabditis elegans C53B4.4b (C53B4.4) mRNA, complete cds. 11/11 None
NM_069158 NCBI Caenorhabditis elegans C53B4.4a (C53B4.4) mRNA, complete cds. 11/11 None
NM_069159 NCBI Caenorhabditis elegans C53B4.4c (C53B4.4) mRNA, complete cds. 11/11 None
NM_001027922 NCBI Caenorhabditis elegans C53B4.4d (C53B4.4) mRNA, complete cds. 11/11 None
SNAP00000003707 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8975617:8984105:1 11/11 None
GENEFINDER00000003717 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8979905:8984105:1 11/11 None
C53B4.4b ENSEMBL cdna:known chromosome:CEL140:IV:8973678:8984686:1 gene:C53B4.4 11/11 A 
C53B4.4a ENSEMBL cdna:known chromosome:CEL140:IV:8973678:8984105:1 gene:C53B4.4 11/11 None
C53B4.4c ENSEMBL cdna:known chromosome:CEL140:IV:8975512:8984105:1 gene:C53B4.4 11/11 None
C53B4.4d ENSEMBL cdna:known chromosome:CEL140:IV:8975617:8984686:1 gene:C53B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8973683-8984111(+) 100.0 100.0
Public Domain and Genome References
Ensembl C53B4.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92456 Hypothetical protein C53B4.4b [Caenorhabditis elegans] ref|NP_501558.1| C53B4.4b [Caenorhabditis elegans] 0.0
blast CAA92457 Hypothetical protein C53B4.4a [Caenorhabditis elegans] ref|NP_501559.1| C53B4.4a [Caenorhabditis elegans] 0.0
blast CAE74079 Hypothetical protein CBG21734 [Caenorhabditis briggsae] 0.0
blast CAB54213 Hypothetical protein C53B4.4c [Caenorhabditis elegans] ref|NP_501560.2| C53B4.4c [Caenorhabditis elegans] 0.0
blast CAH60786 Hypothetical protein C53B4.4d [Caenorhabditis elegans] ref|NP_001023093.1| C53B4.4d [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 2.0E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 2.0E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501558.1 2 110-132,145-164
NP_501559.1 2 110-132,145-164
NP_501560.2 1 2-24
NP_001023093.1 1 2-24
C53B4.4b 2 110-132,145-164
C53B4.4a 2 110-132,145-164
C53B4.4c 1 2-24
C53B4.4d 1 2-24
SNAP00000003707 1 2-24
Sequence
Target Sequence 
>C. ELEGANS:194126_S_AT
ttcaagatgttctctccgatgttcttccaggtctagatggatctccattcataagtggat
tatattttcagcctggaaatgcatataatgagcagacgattcgaaatgcaaaaatgcttg
gacgtgagatcttctgtgatcttccatctgagcaacgagtcgagagaatcaactcacaaa
tcgatagaattcagacagcgataagagaaacgaaagataatcgattgagtgtgatgcaaa
atggcggagacatttcagaatcgtcagccaagtttcaaggtctggacgaacgtcttcaag
ctctcgctgttgtcatgcttcactattgttctgctcttcaagattgccaatcaggaagat
ccacaccagctccaccaccagatgatgataatcagaagagagagattaccgaagaaatat
catccgtcaacgtcgactcaaacaatgaagaagactccgaaagtgttctagatgaggata
cagtaaccggtgacgttccggtgcaacacgatcatccacttgagcgaccaacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAAGATGTTCTCTCCGATGTTCT 626 685 4199 Antisense
TCCGATGTTCTTCCAGGTCTAGATG 687 593 4213 Antisense
ATGCTTGGACGTGAGATCTTCTGTG 55 41 4312 Antisense
TCTTCCATCTGAGCAACGAGTCGAG 472 615 4338 Antisense
GAATCGTCAGCCAAGTTTCAAGGTC 104 329 4456 Antisense
CTGGACGAACGTCTTCAAGCTCTCG 246 241 4480 Antisense
GTCATGCTTCACTATTGTTCTGCTC 314 467 4510 Antisense
AATCAGGAAGATCCACACCAGCTCC 268 171 4547 Antisense
ATATCATCCGTCAACGTCGACTCAA 531 19 4615 Antisense
TGACGTTCCGGTGCAACACGATCAT 696 569 4689 Antisense
ATCATCCACTTGAGCGACCAACAAA 44 27 4709 Antisense
Sequence Source Affymetrix Proprietary Database