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[194125_at] Weak similarity to PAB-dependent poly(A)-specific ribonuclease (Swiss Prot accession number P32521)

Description R04B5.7 /REP_DB=WormBase Gene ID /WP=CE06217 /TR=Q21704 /GB=CAA94843.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Weak similarity to PAB-dependent poly(A)-specific ribonuclease (Swiss Prot accession number P32521)
Cluster 6grp_total > 20      4grp_total > 09      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 10.7
(-2.7)
A 		7.7
(-3)
A 		7.5
(-3)
A 		67.2
(3)
A 		60
B 6.6
(-3)
A 		20.5
(-1.2)
A 		52.4
(3)
A 		29.7
(0)
A 		46
C 29.8
(0.2)
A 		31.2
(0.4)
A 		6.2
(-3)
A 		50.3
(3)
A 		44
avr 15.7
(-3) 		19.8
(-2.3) 		22
(-1.9) 		49.1
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 4.2
(-3)
A 		7.6
(-1.8)
A 		21.2
(3)
A 		7
(-2)
A 		14.3
(0.6)
A 		6.2
(-2.3)
A 		17
B 7.6
(-1.8)
A 		26.7
(3)
A 		8.2
(-1.6)
A 		2.8
(-3)
A 		12.5
(-0.6)
A 		12.7
(-0.5)
A 		24
C 6.9
(-2.6)
A 		31.2
(0.6)
A 		11.6
(-2)
A 		49.2
(3)
A 		4.1
(-3)
A 		5.6
(-2.8)
A 		45
avr 6.2
(-3) 		21.8
(3) 		13.7
(-0.1) 		19.7
(2.2) 		10.3
(-1.4) 		8.2
(-2.2) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 3.9
(-1.4)
A 		1.7
(-3)
A 		2
(-2.8)
A 		9.8
(3)
A 		8
B 2.1
(-3)
A 		5.1
(-1.2)
A 		12.1
(3)
A 		6.3
(-0.5)
A 		10
C 9.9
(3)
A 		9
(2.3)
A 		2.2
(-3)
A 		8.5
(1.9)
A 		8
avr 5.3
(-3) 		5.3
(-3) 		5.4
(-2.8) 		8.2
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 0.8
(-3)
A 		2.7
(0)
A 		4.6
(3)
A 		1.8
(-1.4)
A 		4.3
(2.5)
A 		1.5
(-1.9)
A 		4
B 2.6
(-1.6)
A 		8.9
(3)
A 		2
(-2)
A 		0.7
(-3)
A 		5.1
(0.2)
A 		4.7
(-0.1)
A 		8
C 2.6
(-2.1)
A 		9.5
(2.7)
A 		3
(-1.8)
A 		9.9
(3)
A 		1.3
(-3)
A 		1.9
(-2.6)
A 		9
avr 2
(-3) 		7
(3) 		3.2
(-1.6) 		4.1
(-0.5) 		3.6
(-1.1) 		2.7
(-2.2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194125_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11528
Sequence Type Exemplar sequence
Representative Public ID R04B5.7 NCBI
Target Description R04B5.7 /REP_DB=WormBase Gene ID /WP=CE06217 /TR=Q21704 /GB=CAA94843.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Weak similarity to PAB-dependent poly(A)-specific ribonuclease (Swiss Prot accession number P32521)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073191 NCBI Caenorhabditis elegans R04B5.7 (R04B5.7) mRNA, complete cds. 11/11 None
SNAP00000020538 ENSEMBL cdna:SNAP chromosome:CEL140:V:10091738:10093142:-1 11/11 None
GENEFINDER00000020544 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10091738:10093142:-1 11/11 None
R04B5.7 ENSEMBL cdna:known chromosome:CEL140:V:10091738:10093142:-1 gene:R04B5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10091737-10093142(-) 100.0 100.0
Public Domain and Genome References
Gene Title Weak similarity to PAB-dependent poly(A)-specific ribonuclease (Swiss Prot accession number P32521)
Ensembl R04B5.7
Entrez Gene 187568 Entrez gene
SwissProt Q21704 EMBL-EBI
WormBase CE06217 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94843 Hypothetical protein R04B5.7 [Caenorhabditis elegans] ref|NP_505592.1| R04B5.7 [Caenorhabditis elegans] 0.0
blast CAE64009 Hypothetical protein CBG08604 [Caenorhabditis briggsae] emb|CAE56919.1| Hypothetical protein CBG24764 [Caenorhabditis briggsae] 8.0E-34
Sequence
Target Sequence 
>C. ELEGANS:194125_AT
atacattttcaccaattcctatcaggaagacgataaagaaagaggcaccagttgaatccc
cattggaacatccaaaagttggtttgaattctatgccgattgataacaggcaatatggag
attggaatatgaatcttagtacacacaatactgtttcctggcatgttcctgttccacctg
ctccacagccaatgctgcaggcaacatacccggagcagagaacggtattcggtccttcct
accttcaacaaatggactacaagcatcatgctcaagaactttacaacttctcaactgatt
acttgctcacccttcccccgccacttgggaattcttcaaaccttgccactttgcaatccc
agtccaattggtcaaacaatcaactgtttgtgccaccagcgcatcacatgaatcctgcca
acacacaacaaattcaagttaaaaatgaaccattggactatttggatgaaatgctcttca
atcacaaccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACATTTTCACCAATTCCTATCAG 123 25 356 Antisense
AAGAGGCACCAGTTGAATCCCCATT 632 153 395 Antisense
AATACTGTTTCCTGGCATGTTCCTG 163 173 502 Antisense
TGCTCCACAGCCAATGCTGCAGGCA 624 579 534 Antisense
CAGAGAACGGTATTCGGTCCTTCCT 545 197 571 Antisense
GTCCTTCCTACCTTCAACAAATGGA 466 477 587 Antisense
TTCTCAACTGATTACTTGCTCACCC 628 689 643 Antisense
TCAAACCTTGCCACTTTGCAATCCC 571 629 691 Antisense
ACAATCAACTGTTTGTGCCACCAGC 1 115 731 Antisense
CCAGCGCATCACATGAATCCTGCCA 94 273 751 Antisense
GAAATGCTCTTCAATCACAACCAAA 18 363 823 Antisense
Sequence Source Affymetrix Proprietary Database