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[194124_s_at]

Description C04H5.4A /REP_DB=WormBase Gene ID /WP=CE23518 /TR=Q9U3R7 /GB=CAB54180.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Adenylate and Guanylate cyclase catalytic domain, and eukaryotic protein kinase domain (2 domains)
Cluster 6grp_total > 06      4grp_total > 12      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 97
(-0.1)
P 		34.2
(-2.2)
A 		12.1
(-3)
A 		187.4
(3)
A 		175
B 68.8
(-3)
A 		80.9
(-1.3)
A 		73.3
(-2.4)
A 		112.3
(3)
P 		44
C 89.5
(-0.9)
A 		41.1
(-2.7)
A 		33.5
(-3)
A 		194.6
(3)
P 		161
avr 85.1
(-0.8) 		52.1
(-2.4) 		39.6
(-3) 		164.8
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 75.3
(3)
A 		45.6
(0.4)
A 		44.7
(0.3)
A 		7.4
(-3)
A 		50.9
(0.8)
A 		35
(-0.6)
A 		68
B 98.3
(3)
P 		68.3
(0.4)
A 		42.7
(-1.8)
A 		28.5
(-3)
A 		59.6
(-0.3)
A 		37
(-2.3)
A 		70
C 79.8
(3)
A 		71.1
(1.9)
A 		54.1
(-0.4)
A 		72.4
(2)
A 		55.4
(-0.2)
A 		34.2
(-3)
A 		46
avr 84.5
(3) 		61.7
(0.2) 		47.2
(-1.6) 		36.1
(-2.9) 		55.3
(-0.6) 		35.4
(-3) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 35.3
(3)
P 		7.4
(-2.2)
A 		3.2
(-3)
A 		27.4
(1.5)
A 		32
B 22
(1.3)
A 		20
(-0.4)
A 		17
(-3)
A 		23.9
(3)
P 		7
C 29.6
(2.1)
A 		11.8
(-3)
A 		11.7
(-3)
A 		32.8
(3)
P 		21
avr 29
(3) 		13.1
(-2.2) 		10.6
(-3) 		28
(2.7) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 13.9
(2)
A 		16.2
(3)
A 		9.7
(0.3)
A 		1.9
(-3)
A 		15.4
(2.7)
A 		8.5
(-0.2)
A 		14
B 34.2
(3)
P 		22.7
(0.4)
A 		10.4
(-2.3)
A 		7.5
(-3)
A 		24.2
(0.8)
A 		13.8
(-1.6)
A 		27
C 29.6
(3)
A 		21.7
(0.4)
A 		13.8
(-2.2)
A 		14.6
(-2)
A 		17.9
(-0.9)
A 		11.5
(-3)
A 		18
avr 25.9
(3) 		20.2
(1.1) 		11.3
(-1.9) 		8
(-3) 		19.2
(0.8) 		11.3
(-1.9) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194124_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3430
Sequence Type Exemplar sequence
Representative Public ID C04H5.4A NCBI
Target Description C04H5.4A /REP_DB=WormBase Gene ID /WP=CE23518 /TR=Q9U3R7 /GB=CAB54180.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Adenylate and Guanylate cyclase catalytic domain, and eukaryotic protein kinase domain (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064625(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064625 NCBI Caenorhabditis elegans C04H5.3 (C04H5.3) mRNA, complete cds. 11/11 A 
SNAP00000026417 ENSEMBL cdna:SNAP chromosome:CEL140:II:14540801:14548088:-1 11/11 None
GENEFINDER00000026422 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14535050:14548665:-1 11/11 None
C04H5.3 ENSEMBL cdna:known chromosome:CEL140:II:14540786:14548682:-1 gene:C04H5.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14540797-14543703(-) 99.5 100.0
Public Domain and Genome References
Gene Title
Ensembl C04H5.3
Entrez Gene 175116 Entrez gene
SwissProt O62026 EMBL-EBI
WormBase CE32791 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03842 Hypothetical protein C04H5.3 [Caenorhabditis elegans] ref|NP_497026.2| C04H5.3 [Caenorhabditis elegans] 0.0
blast T18932 hypothetical protein C04H5.4a - Caenorhabditis elegans 0.0
blast NP_500309 Guanylyl CYclase family member (gcy-23) [Caenorhabditis elegans] gb|AAC17555.3| Guanylyl cyclase protein 23 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 5.7E-28
Pfam IPR000719 EMBL-EBI Protein kinase 0.16
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-8
Pfam IPR001054 EMBL-EBI Guanylate cyclase 8.4E-66
Pfam IPR000719 EMBL-EBI Protein kinase 0.16
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-8
Pfam IPR001054 EMBL-EBI Guanylate cyclase 8.4E-66
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 5.7E-28
Pfam IPR000719 EMBL-EBI Protein kinase 0.16
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-8
Pfam IPR001054 EMBL-EBI Guanylate cyclase 8.4E-66
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497026.2 2 5-22,425-444
C04H5.3 2 5-22,425-444
GENEFINDER00000026422 2 5-22,460-479
Sequence
Target Sequence 
>C. ELEGANS:194124_S_AT
tgaaagccggacggaccgttgcaccaaaaatgtacgactcggcaacagtgatgttcagcg
atattgtgggcttcactaaactttgctcagcttccacccctatagaggtcgtgaacctcc
tcaacaaactctactcggaattcgacactgtaatttcaaagcatgactgctataaggttg
agacaattggagacgcctatatggttgtttctggaattcccattgaaaatgggcagcgcc
acgtggcaaacatctcagcggtcaccttgggaattatggacctactgaaggtgtttgagg
tgccacaccgaagagattatcgtctgacgattcgattgggattcgcgtcaggacaagttt
cggcggcagttgttgggttgagctctccgagatattgtctatttggggaaaccgttaaca
ttgcggcggtcatggaatcttctggggaaggcggccgagttcagataacagaaacgtcga
agatacttctggagaacgagtatccagagtttattatagagatccgtggaattaataagg
atgtcaaacaagacgactttgtcacatactggctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAGCCGGACGGACCGTTGCACC 388 571 977 Antisense
TGTGGGCTTCACTAAACTTTGCTCA 150 553 1041 Antisense
CTTCCACCCCTATAGAGGTCGTGAA 21 219 1067 Antisense
CCTCCTCAACAAACTCTACTCGGAA 497 267 1092 Antisense
GAGACGCCTATATGGTTGTTTCTGG 637 393 1166 Antisense
CGCCACGTGGCAAACATCTCAGCGG 559 257 1213 Antisense
CATCTCAGCGGTCACCTTGGGAATT 254 211 1227 Antisense
GCGTCAGGACAAGTTTCGGCGGCAG 565 291 1321 Antisense
CGGCAGTTGTTGGGTTGAGCTCTCC 676 241 1340 Antisense
ACATTGCGGCGGTCATGGAATCTTC 157 105 1394 Antisense
GACGACTTTGTCACATACTGGCTCA 540 379 1528 Antisense
Sequence Source Affymetrix Proprietary Database