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[194109_s_at] Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain

Description Y75B12B.6 /REP_DB=WormBase Gene ID /WP=CE20375 /TR=Q9XWB7 /GB=CAA21765.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 77      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 81      6grp_total > 77      4grp_total > 12      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 328.1
(3)
P 		215.1
(-1.3)
P 		170.8
(-3)
P 		318.6
(2.6)
P 		157
B 216.7
(-3)
P 		224.2
(-2.6)
P 		232.5
(-2.1)
P 		323.7
(3)
P 		107
C 229.3
(-2.7)
P 		221.5
(-3)
P 		238.5
(-2.3)
P 		363
(3)
P 		142
avr 258
(-0.8) 		220.3
(-2.7) 		213.9
(-3) 		335.1
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 182.9
(-3)
P 		283.5
(2)
P 		203.4
(-2)
P 		304.4
(3)
P 		210.9
(-1.6)
P 		293.9
(2.5)
P 		122
B 244.2
(-1.2)
P 		223.4
(-2.5)
P 		214.9
(-3)
P 		239.4
(-1.5)
P 		231.9
(-2)
P 		313.5
(3)
P 		99
C 244.8
(-0.8)
P 		204.6
(-2.5)
P 		192.1
(-3)
P 		198.6
(-2.7)
P 		238.2
(-1.1)
P 		335.6
(3)
P 		144
avr 224
(-1.9) 		237.2
(-1.2) 		203.5
(-3) 		247.5
(-0.6) 		227
(-1.7) 		314.3
(3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 119.5
(3)
P 		46.7
(-2.8)
P 		44.6
(-3)
P 		46.6
(-2.8)
P 		75
B 69.1
(3)
P 		55.4
(-2.4)
P 		53.9
(-3)
P 		69
(3)
P 		15
C 75.9
(1)
P 		63.7
(-2.3)
P 		83.1
(3)
P 		61.2
(-3)
P 		22
avr 88.2
(3) 		55.3
(-3) 		60.5
(-2.1) 		58.9
(-2.3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 33.7
(-3)
P 		100.8
(3)
P 		44.3
(-2.1)
P 		77.2
(0.9)
P 		63.7
(-0.3)
P 		71.5
(0.4)
P 		67
B 85
(0)
P 		74.4
(-0.9)
P 		52.2
(-3)
P 		63.4
(-2)
P 		94.2
(0.9)
P 		116.9
(3)
P 		65
C 90.7
(1.2)
P 		62.4
(-1.1)
P 		48.9
(-2.3)
P 		39.9
(-3)
P 		77.1
(0.1)
P 		112.4
(3)
P 		73
avr 69.8
(-0.5) 		79.2
(0.6) 		48.5
(-3) 		60.2
(-1.6) 		78.3
(0.5) 		100.3
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194109_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14036
Sequence Type Exemplar sequence
Representative Public ID Y75B12B.6 NCBI
Target Description Y75B12B.6 /REP_DB=WormBase Gene ID /WP=CE20375 /TR=Q9XWB7 /GB=CAA21765.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074351(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074351 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-2) (plc-2) mRNA, complete cds. 11/11 A 
SNAP00000027061 ENSEMBL cdna:SNAP chromosome:CEL140:V:15197607:15200156:1 11/11 None
GENEFINDER00000027065 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15195309:15203807:1 11/11 None
Y75B12B.6 ENSEMBL cdna:known chromosome:CEL140:V:15195254:15200247:1 gene:Y75B12B.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15195308-15200156(+) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphatidylinositol-specific phospholipase C, X domain, Phosphatidylinositol-specific phospholipase C, Y domain
Gene Symbol plc-2
Ensembl Y75B12B.6
Entrez Gene 180029 Entrez gene
SwissProt Q9XWB7 EMBL-EBI
WormBase CE20375 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4435 phosphoinositide phospholipase C activity inferred from electronic annotation QuickGO AmiGO
4629 phospholipase C activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21765 Hypothetical protein Y75B12B.6 [Caenorhabditis elegans] ref|NP_506752.1| PhosphoLipase C family member (plc-2) [Caenorhabditis elegans] 0.0
blast CAE71603 Hypothetical protein CBG18562 [Caenorhabditis briggsae] 0.0
blast CAA21766 Hypothetical protein Y75B12B.7 [Caenorhabditis elegans] ref|NP_506753.2| Serpentine Receptor, class W family member (srw-1) [Caenorhabditis elegans] 2.0E-90
blast CAG00327 unnamed protein product [Tetraodon nigroviridis] 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 5.9E-55
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 1.8E-28
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 5.9E-55
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 1.8E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027065 2 1027-1049,1070-1092
Sequence
Target Sequence 
>C. ELEGANS:194109_S_AT
gggaacgaagattcagcaatccatttgcagacctggacaagtttgatgacaatgagttcc
ttctagtagagggaacacgcagaggatcagtggagcatgcaccaacaacacccaccacac
caaccaagccaatgtcttttgggaaggagattctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGAACGAAGATTCAGCAATCCATT 56 495 2519 Antisense
GATTCAGCAATCCATTTGCAGACCT 375 431 2528 Antisense
GCAATCCATTTGCAGACCTGGACAA 413 321 2534 Antisense
GCAGACCTGGACAAGTTTGATGACA 54 313 2545 Antisense
GTTTGATGACAATGAGTTCCTTCTA 683 449 2559 Antisense
TGACAATGAGTTCCTTCTAGTAGAG 383 569 2565 Antisense
GTAGAGGGAACACGCAGAGGATCAG 692 455 2584 Antisense
GAGGATCAGTGGAGCATGCACCAAC 455 403 2600 Antisense
CCACACCAACCAAGCCAATGTCTTT 92 275 2633 Antisense
CCAACCAAGCCAATGTCTTTTGGGA 374 277 2638 Antisense
AATGTCTTTTGGGAAGGAGATTCTG 694 161 2649 Antisense
Sequence Source Affymetrix Proprietary Database