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[194093_s_at]

Description F48E3.1 /REP_DB=WormBase Gene ID /WP=CE28401 /GEN=gly-12 /TR=Q9GPA3 /GB=AAF99958.3 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase I
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 10      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 61      6grp_total > 41      4grp_total > 51      6grp_highest_sub > 04      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 356
(-2.7)
P 		510.3
(3)
P 		390.4
(-1.4)
P 		346.7
(-3)
P 		164
B 336
(-2.3)
P 		488.9
(3)
P 		315.9
(-3)
P 		360.9
(-1.4)
P 		173
C 489
(3)
P 		385.2
(-1.2)
P 		467.5
(2.1)
P 		340.6
(-3)
P 		148
avr 393.7
(-0.6) 		461.5
(3) 		391.3
(-0.8) 		349.4
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 429.8
(2.5)
P 		433.6
(2.6)
P 		384.7
(1.2)
P 		450.2
(3)
P 		296.9
(-1.1)
P 		225.9
(-3)
P 		224
B 382.8
(1.1)
P 		421.4
(2)
P 		425.7
(2.1)
P 		465.7
(3)
P 		296.7
(-0.9)
P 		202.9
(-3)
P 		263
C 434.9
(2.9)
P 		353.7
(0.7)
P 		440
(3)
P 		437.2
(2.9)
P 		370.8
(1.1)
P 		215.8
(-3)
P 		224
avr 415.8
(2.1) 		402.9
(1.8) 		416.8
(2.1) 		451
(3) 		321.5
(-0.3) 		214.9
(-3) 		236
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 129.6
(3)
P 		110.8
(1.6)
P 		102
(0.9)
P 		50.7
(-3)
P 		79
B 107.1
(1.3)
P 		120.7
(3)
P 		73.2
(-3)
P 		76.9
(-2.5)
P 		48
C 161.9
(2.9)
P 		110.8
(0)
P 		162.9
(3)
P 		57.4
(-3)
P 		106
avr 132.9
(3) 		114.1
(1.4) 		112.7
(1.3) 		61.7
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 79.2
(-1.5)
P 		154.2
(3)
P 		83.8
(-1.3)
P 		114.2
(0.6)
P 		89.6
(-0.9)
P 		55
(-3)
P 		99
B 133.2
(2.3)
P 		140.3
(3)
P 		103.4
(-0.4)
P 		123.4
(1.4)
P 		120.5
(1.2)
P 		75.7
(-3)
P 		65
C 161.2
(3)
P 		107.9
(-0.6)
P 		112
(-0.3)
P 		87.9
(-1.9)
P 		120
(0.2)
P 		72.2
(-3)
P 		89
avr 124.5
(2.1) 		134.1
(3) 		99.7
(-0.1) 		108.5
(0.7) 		110
(0.8) 		67.6
(-3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194093_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16890
Sequence Type Exemplar sequence
Representative Public ID F48E3.1 NCBI
Target Description F48E3.1 /REP_DB=WormBase Gene ID /WP=CE28401 /GEN=gly-12 /TR=Q9GPA3 /GB=AAF99958.3 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase I
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171727(11), NM_001029520(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171727 NCBI Caenorhabditis elegans GLYcosylation related family member (gly-12) (gly-12) mRNA, complete cds. 11/11 A 
NM_001029520 NCBI Caenorhabditis elegans GLYcosylation related family member (gly-12) (gly-12) mRNA, complete cds. 11/11 None
SNAP00000040269 ENSEMBL cdna:SNAP chromosome:CEL140:X:7486117:7488720:1 9/11 None
GENEFINDER00000040277 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7486117:7490367:1 9/11 None
F48E3.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:7485809:7490641:1 gene:F48E3.1 11/11 A 
F48E3.1b ENSEMBL cdna:known chromosome:CEL140:X:7486012:7490645:1 gene:F48E3.1 11/11 A 
F48E3.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:7486117:7490640:1 gene:F48E3.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7486115-7488719(+) 100.0 100.0
Public Domain and Genome References
Ensembl F48E3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99958 Glycosylation related protein 12, isoform a [Caenorhabditis elegans] ref|NP_741838.1| GLYcosylation related family member (gly-12) [Caenorhabditis elegans] gb|AAD03023.1| UDP-N-acetylglucosamine:a-3-D-mannoside b-1,2-N-acetylglucosaminyltransferase I [Caenorhabditis elegans] 0.0
blast AAM69111 Glycosylation related protein 12, isoform b [Caenorhabditis elegans] ref|NP_001024691.1| GLYcosylation related family member (gly-12) [Caenorhabditis elegans] 0.0
blast CAE70220 Hypothetical protein CBG16700 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 3.6E-97
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.8E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741838.1 1 13-32
NP_001024691.1 1 13-32
F48E3.1a.2 1 13-32
F48E3.1b 1 13-32
F48E3.1a.1 1 13-32
SNAP00000040269 1 13-32
GENEFINDER00000040277 1 13-32
Sequence
Target Sequence 
>C. ELEGANS:194093_S_AT
caggtcatgtatccgtccggaaatcagtagaacgtcgcataacatgaagttagctggaaa
agggtctagtggaggtatgttcaaagactatctcagcaaaattagcgcatccagcgcaaa
cattgacttctctcttctccctgtcaccctcgtccaaaaatcaatctacgacaagcgcct
tatagaacaaattgagaatgcacgacccatagacctgcagaacactactgggatggagaa
aacctacaattacaaaattgtgtacaagaacattcgagactggcataggctggccgccca
tttcaaattgatgacagacattcgatatcacattaagggaggaatgcagcgaacagcata
ctatggagttgtcactttgatgttccagaattgcagagtgtttttggtacctgaatcaac
gtaccgaaatccatcacaacttactagttacgtctacgattcagaatgggacaaacaaaa
ccgatttattgaatttgaagcatattactgcaaaactaaaaaatatgctggaaaatgcga
cccacacagtcccgagatgattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGTCATGTATCCGTCCGGAAATC 10 207 801 Antisense
AGCGCAAACATTGACTTCTCTCTTC 104 83 913 Antisense
TCACCCTCGTCCAAAAATCAATCTA 686 625 944 Antisense
ATCAATCTACGACAAGCGCCTTATA 699 25 960 Antisense
GACCCATAGACCTGCAGAACACTAC 564 381 1004 Antisense
TCGAGACTGGCATAGGCTGGCCGCC 474 599 1074 Antisense
CTGGCCGCCCATTTCAAATTGATGA 199 241 1090 Antisense
GAGTTGTCACTTTGATGTTCCAGAA 609 397 1166 Antisense
AACGTACCGAAATCCATCACAACTT 144 143 1218 Antisense
TATGCTGGAAAATGCGACCCACACA 338 657 1324 Antisense
GACCCACACAGTCCCGAGATGATTG 458 381 1339 Antisense
Sequence Source Affymetrix Proprietary Database