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[194086_at] Kunitz/Bovine pancreatic trypsin inhibitor domain (10 domains), Thyroglobulin type-1 repeat

Description W01F3.3 /REP_DB=WormBase Gene ID /WP=CE16531 /TR=O45881 /GB=CAB07294.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Kunitz-Bovine pancreatic trypsin inhibitor domain (10 domains), Thyroglobulin type-1 repeat
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      6grp_highest_nonorm > 40      6grp_total > 04      4grp_total > 49      6grp_cgc > 41      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1117
1 2 3 4 range
A 37.4
(-2.8)
A 		999.5
(3)
P 		870.1
(2.2)
P 		12.5
(-3)
A 		987
B 11.3
(-3)
A 		806.9
(2.1)
P 		944.2
(3)
P 		12
(-3)
A 		933
C 9.7
(-3)
A 		1126.3
(3)
P 		919
(1.9)
P 		40.8
(-2.8)
A 		1117
avr 19.5
(-3) 		977.6
(3) 		911.1
(2.6) 		21.8
(-3) 		958

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1252
1 2 3 4 5 6 range
A 778.9
(3)
P 		701.2
(2.2)
P 		373
(-1.4)
P 		562.5
(0.6)
P 		491.3
(-0.1)
P 		226.6
(-3)
P 		552
B 845.2
(3)
P 		746.6
(2.1)
P 		425
(-0.8)
P 		754.7
(2.2)
P 		327
(-1.6)
P 		174
(-3)
P 		671
C 1374.3
(3)
P 		699.2
(-0.2)
P 		442.7
(-1.5)
P 		560.4
(-0.9)
P 		571.2
(-0.8)
P 		122.7
(-3)
P 		1252
avr 999.5
(3) 		715.7
(0.9) 		413.6
(-1.3) 		625.9
(0.3) 		463.2
(-0.9) 		174.4
(-3) 		825
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 13.6
(-2.7)
A 		217
(2.7)
P 		227.4
(3)
P 		1.8
(-3)
A 		226
B 3.6
(-3)
A 		199.3
(2.5)
P 		218.8
(3)
P 		2.6
(-3)
A 		216
C 3.2
(-3)
A 		323.8
(3)
P 		320.3
(2.9)
P 		6.9
(-2.9)
A 		321
avr 6.8
(-2.9) 		246.7
(2.8) 		255.5
(3) 		3.8
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 468
1 2 3 4 5 6 range
A 143.6
(-0.3)
P 		249.4
(3)
P 		81.2
(-2.2)
P 		142.7
(-0.3)
P 		148.3
(-0.1)
P 		55.2
(-3)
P 		194
B 294.1
(3)
P 		248.5
(1.8)
P 		103.2
(-2)
P 		199.9
(0.5)
P 		132.9
(-1.2)
P 		64.9
(-3)
P 		229
C 509.4
(3)
P 		213.3
(-0.8)
P 		112.7
(-2.1)
P 		112.7
(-2.1)
P 		184.8
(-1.2)
P 		41.1
(-3)
P 		468
avr 315.7
(3) 		237.1
(1.2) 		99
(-2) 		151.8
(-0.8) 		155.3
(-0.7) 		53.7
(-3) 		262
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194086_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13721
Sequence Type Exemplar sequence
Representative Public ID W01F3.3 NCBI
Target Description W01F3.3 /REP_DB=WormBase Gene ID /WP=CE16531 /TR=O45881 /GB=CAB07294.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Kunitz-Bovine pancreatic trypsin inhibitor domain (10 domains), Thyroglobulin type-1 repeat
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075592(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075592 NCBI Caenorhabditis elegans W01F3.3 (W01F3.3) mRNA, complete cds. 11/11 None
SNAP00000024490 ENSEMBL cdna:SNAP chromosome:CEL140:V:20665285:20676211:1 11/11 None
GENEFINDER00000024491 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20665285:20679891:1 11/11 None
W01F3.3 ENSEMBL cdna:known chromosome:CEL140:V:20663308:20676528:1 gene:W01F3.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20663307-20676353(+) 97.54 100.0
Public Domain and Genome References
Gene Title Kunitz/Bovine pancreatic trypsin inhibitor domain (10 domains), Thyroglobulin type-1 repeat
Gene Symbol mlt-11
Ensembl W01F3.3
Entrez Gene 180352 Entrez gene
SwissProt O45881 EMBL-EBI
WormBase CE16531 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07294 Hypothetical protein W01F3.3 [Caenorhabditis elegans] ref|NP_507993.1| W01F3.3 [Caenorhabditis elegans] 0.0
blast CAE61690 Hypothetical protein CBG05636 [Caenorhabditis briggsae] 0.0
blast AAN10061 Kunitz-like protease inhibitor precursor [Ancylostoma caninum] 2.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR000716 EMBL-EBI Thyroglobulin type-1 3.0E-18
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.6E-23
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.6E-27
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 6.1E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 7.0E-25
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.0E-26
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 4.9E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.0E-26
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 3.4E-21
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 9.8E-30
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.3E-16
Pfam IPR000716 EMBL-EBI Thyroglobulin type-1 3.0E-18
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.6E-23
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.6E-27
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 6.1E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 7.0E-25
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.0E-26
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 4.9E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.0E-26
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 3.4E-21
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 9.8E-30
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.3E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000024491 3 3197-3219,3232-3254,3269-3291
Sequence
Target Sequence 
>C. ELEGANS:194086_AT
gtgccccaagggatactattgcgcaattgattctgcaagaaattctagattgtgttgccc
actgtatggagatgctcaaagaatcgcctctgaggagatctttgcaccacgactagcctc
caacacagaaacaaccacagaggctaaggttgagaacattgacgttgaagaatcagaaga
tgatgaggaggaggatggagaagactttgtggctcaccttcaaatgaagccaaacaagcc
tgtaaatcaggttgaggagcttgccaagagttctccaatcgcagcagatttgagtgcaga
gggaggagtgtccattgatttgggtgctgatgagaagaaggaagttgaagaggaggatgt
gacgacgaccactgaaaaaatgatggttcaggacaagagtgtttgtcagattaagccatc
ggaaggacgcgtctgtaacgattccgaaacaccaacacgcacgaaccttcaatacttcta
ctccccacgtgacaaccgttgtaagctcttcttcttccgtggatgtggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCCCAAGGGATACTATTGCGCA 597 477 6078 Antisense
GAGATGCTCAAAGAATCGCCTCTGA 145 397 6146 Antisense
TCTGAGGAGATCTTTGCACCACGAC 595 607 6166 Antisense
GACTAGCCTCCAACACAGAAACAAC 212 373 6188 Antisense
AAGACTTTGTGGCTCACCTTCAAAT 200 153 6278 Antisense
GCTTGCCAAGAGTTCTCCAATCGCA 18 307 6336 Antisense
TAAGCCATCGGAAGGACGCGTCTGT 456 637 6489 Antisense
AACGATTCCGAAACACCAACACGCA 143 145 6514 Antisense
GCACGAACCTTCAATACTTCTACTC 484 313 6536 Antisense
ACAACCGTTGTAAGCTCTTCTTCTT 302 113 6569 Antisense
CTCTTCTTCTTCCGTGGATGTGGAG 492 229 6583 Antisense
Sequence Source Affymetrix Proprietary Database