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[194083_s_at]

Description T25E12.4A /REP_DB=WormBase Gene ID /WP=CE18967 /TR=O45818 /GB=CAB04830.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phorbol esters-diacylglycerol binding domain (C1 domain) (2 domains), serine-threonine kinase
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 18      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 50      6grp_total > 18      4grp_total > 23      6grp_highest_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 425
1 2 3 4 range
A 695.7
(2.4)
P 		335.7
(-3)
P 		418.8
(-1.8)
P 		735.8
(3)
P 		400
B 760.9
(3)
P 		625.1
(0.2)
P 		473.8
(-3)
P 		608.7
(-0.2)
P 		287
C 647.4
(3)
P 		484
(-3)
P 		642.5
(2.8)
P 		644.7
(2.9)
P 		163
avr 701.3
(3) 		481.6
(-3) 		511.7
(-2.2) 		663.1
(2) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 735
1 2 3 4 5 6 range
A 632.5
(3)
P 		564.9
(1.9)
P 		254.4
(-3)
P 		597.8
(2.4)
P 		490.7
(0.7)
P 		618.1
(2.8)
P 		378
B 497.2
(3)
P 		434.7
(1.5)
P 		252.6
(-3)
P 		375.2
(0)
P 		461.1
(2.1)
P 		402.1
(0.7)
P 		245
C 987.5
(3)
P 		375.8
(-2.2)
P 		570.9
(-0.5)
P 		311.3
(-2.7)
P 		553.8
(-0.7)
P 		279.5
(-3)
P 		708
avr 705.7
(3) 		458.5
(-1.3) 		359.3
(-3) 		428.1
(-1.8) 		501.9
(-0.5) 		433.2
(-1.7) 		346
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 253.3
(3)
P 		72.9
(-3)
P 		109.5
(-1.8)
P 		107.7
(-1.8)
P 		180
B 242.6
(3)
P 		154.4
(-1)
P 		109.8
(-3)
P 		129.7
(-2.1)
P 		133
C 214.3
(2.5)
P 		139.1
(-1.4)
P 		223.9
(3)
P 		108.7
(-3)
P 		115
avr 236.7
(3) 		122.1
(-2.7) 		147.7
(-1.4) 		115.4
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 311
1 2 3 4 5 6 range
A 116.6
(-0.5)
P 		200.9
(3)
P 		55.4
(-3)
P 		151.6
(1)
P 		148.1
(0.8)
P 		150.5
(0.9)
P 		146
B 173
(2.3)
P 		144.7
(1)
P 		61.3
(-3)
P 		99.4
(-1.2)
P 		187.3
(3)
P 		150
(1.2)
P 		126
C 366
(3)
P 		114.7
(-2)
P 		145.3
(-1.4)
P 		62.6
(-3)
P 		179.2
(-0.7)
P 		93.6
(-2.4)
P 		303
avr 218.5
(3) 		153.4
(0) 		87.3
(-3) 		104.5
(-2.2) 		171.5
(0.9) 		131.4
(-1) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194083_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14395
Sequence Type Exemplar sequence
Representative Public ID T25E12.4A NCBI
Target Description T25E12.4A /REP_DB=WormBase Gene ID /WP=CE18967 /TR=O45818 /GB=CAB04830.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phorbol esters-diacylglycerol binding domain (C1 domain) (2 domains), serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074838(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074838 NCBI Caenorhabditis elegans T25E12.4a (T25E12.4) mRNA, complete cds. 11/11 A 
T25E12.4a ENSEMBL cdna:known chromosome:CEL140:V:16750140:16790995:1 gene:T25E12.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017337 3/11 Cross Hyb Matching Probes None
GENEFINDER00000017341 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180121 2 1 NM_074838 194083_s_at (11)
NM_074839 185595_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16750202-16790708(+) 100.0 100.0
Public Domain and Genome References
Ensembl T25E12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07478 Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAB04830.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAA16519.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] emb|CAA16430.1| Hypothetical protein T25E12.4a [Caenorhabditis elegans] ref|NP_507239.1| T25E12.4a [Caenorhabditis elegans] 0.0
blast NP_005804 protein kinase D3 [Homo sapiens] gb|AAY14817.1| unknown [Homo sapiens] sp|O94806|KPCN_HUMAN Protein kinase C, nu type (nPKC-nu) (Protein kinase EPK2) dbj|BAA36514.1| serine/threonine kinase [Homo sapiens] 0.0
blast XP_540151 PREDICTED: similar to Protein kinase C, nu type (nPKC-nu) (Protein kinase EPK2) [Canis familiaris] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-60
hanks 1.2.18 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCm 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 3.2E-17
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 7.8E-21
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-80
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.5E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507239.1 1 1068-1090
T25E12.4a 1 1068-1090
Sequence
Target Sequence 
>C. ELEGANS:194083_S_AT
agaatatccttctcaactcgaactcggacttcccacaagtcaagctttgcgatttcggat
tcgctcgaatcattggagagaagagcttcaggcggagtgtcgtcggaacgcctgcctacc
tggcgcctgaagttctacggaacaagggattcaatcgatcgctcgacatgtggagtgtcg
gtgtgattgtctacgtctcactgtcgggaacgttcccgttcaacgaggacgaggatatca
acgatcagattcagaacgccgaattcatgtacccgccgacaccgtggaaggagatctctg
agaatgctatcgaattcatcaacggactgctccaagttaagatgagcaagcggtatacgg
tgacgaaagcgcagtcgcagatctggatgcagaactacaccctatggtcggatctacgag
tactggaaaaagctgtcggtcaacgattcgtaactcatgaaagtgatgatcagcaggacc
cccagagaaacttttcatattttttgcatcttcttgtcacatatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAATATCCTTCTCAACTCGAACTC 198 75 2702 Antisense
GAAGAGCTTCAGGCGGAGTGTCGTC 499 339 2781 Antisense
AGTGTCGGTGTGATTGTCTACGTCT 192 59 2875 Antisense
TACGTCTCACTGTCGGGAACGTTCC 495 645 2893 Antisense
TCAGAACGCCGAATTCATGTACCCG 680 623 2952 Antisense
CCCGCCGACACCGTGGAAGGAGATC 563 259 2973 Antisense
TCGAATTCATCAACGGACTGCTCCA 429 601 3011 Antisense
AGCGCAGTCGCAGATCTGGATGCAG 195 75 3069 Antisense
ACTACACCCTATGGTCGGATCTACG 424 101 3095 Antisense
CAGGACCCCCAGAGAAACTTTTCAT 19 179 3175 Antisense
TTTTTGCATCTTCTTGTCACATATC 190 675 3202 Antisense
Sequence Source Affymetrix Proprietary Database