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[194075_s_at]

Description ZK328.1 /REP_DB=WormBase Gene ID /WP=CE05065 /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin conjugating enzyme; recoverin subfamily of EF-hand calcium binding protein (ST.LOUIS) TR:Q23466 protein_id:AAA91251.1
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 45      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 51      6grp_total > 12      4grp_total > 14      6grp_highest_sub > 02      6grp_cgc > 40      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 395
1 2 3 4 range
A 656.2
(0.9)
P 		599.8
(-0.5)
P 		500.2
(-3)
P 		743.1
(3)
P 		243
B 762.5
(1.8)
P 		449.3
(-3)
P 		478.2
(-2.6)
P 		844.1
(3)
P 		395
C 623.8
(2)
P 		505.8
(-3)
P 		512.5
(-2.7)
P 		648.5
(3)
P 		143
avr 680.8
(1.4) 		518.3
(-2.5) 		497
(-3) 		745.2
(3) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 306
1 2 3 4 5 6 range
A 553
(-0.2)
P 		685.3
(3)
P 		437.4
(-3)
P 		679.1
(2.8)
P 		506.5
(-1.3)
P 		577.9
(0.4)
P 		248
B 697.1
(2.7)
P 		713
(3)
P 		607.4
(0.9)
P 		656.7
(1.9)
P 		411.9
(-3)
P 		538.5
(-0.5)
P 		301
C 656.6
(1.4)
P 		681.5
(2.1)
P 		572.9
(-0.7)
P 		718.3
(3)
P 		482.4
(-3)
P 		523.5
(-2)
P 		236
avr 635.6
(1.5) 		693.3
(3) 		539.2
(-1.1) 		684.7
(2.8) 		466.9
(-3) 		546.6
(-0.9) 		226
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 238.9
(3)
P 		130.2
(-2)
P 		130.8
(-2)
P 		108.8
(-3)
P 		130
B 243.2
(3)
P 		111
(-3)
P 		110.8
(-3)
P 		179.9
(0.1)
P 		132
C 206.5
(3)
P 		145.4
(-0.8)
P 		178.6
(1.3)
P 		109.3
(-3)
P 		97
avr 229.5
(3) 		128.9
(-3) 		140.1
(-2.3) 		132.7
(-2.8) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 101.9
(-2.7)
P 		243.7
(3)
P 		95.3
(-3)
P 		172.2
(0.1)
P 		152.8
(-0.7)
P 		140.7
(-1.2)
P 		148
B 242.6
(3)
P 		237.3
(2.7)
P 		147.5
(-3)
P 		173.9
(-1.3)
P 		167.3
(-1.8)
P 		200.8
(0.4)
P 		95
C 243.4
(3)
P 		207.9
(0.8)
P 		145.8
(-2.9)
P 		144.4
(-3)
P 		156.1
(-2.3)
P 		175.2
(-1.1)
P 		99
avr 196
(1) 		229.6
(3) 		129.5
(-3) 		163.5
(-1) 		158.7
(-1.2) 		172.2
(-0.4) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194075_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6038
Sequence Type Exemplar sequence
Representative Public ID ZK328.1 NCBI
Target Description ZK328.1 /REP_DB=WormBase Gene ID /WP=CE05065 /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin conjugating enzyme; recoverin subfamily of EF-hand calcium binding protein (ST.LOUIS) TR:Q23466 protein_id:AAA91251.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065910(11), NM_001027821(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065910 NCBI Caenorhabditis elegans CYtoKinesis defect family member (cyk-3) (cyk-3) mRNA, complete cds. 11/11 None
NM_001027821 NCBI Caenorhabditis elegans CYtoKinesis defect family member (cyk-3) (cyk-3) mRNA, complete cds. 11/11 None
SNAP00000038059 ENSEMBL cdna:SNAP chromosome:CEL140:III:6020154:6024361:1 11/11 None
GENEFINDER00000038070 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6020154:6024361:1 11/11 None
ZK328.1a ENSEMBL cdna:known chromosome:CEL140:III:6020107:6024580:1 gene:ZK328.1 11/11 None
ZK328.1b ENSEMBL cdna:known chromosome:CEL140:III:6020154:6024361:1 gene:ZK328.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6020152-6024360(+) 99.97 99.97
Public Domain and Genome References
Ensembl ZK328.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL32262 Cytokinesis defect protein 3, isoform a [Caenorhabditis elegans] ref|NP_498311.2| CYtoKinesis defect family member (cyk-3) [Caenorhabditis elegans] gb|AAL79016.1| ubiquitin c-terminal hydrolase [Caenorhabditis elegans] 0.0
blast AAL32263 Cytokinesis defect protein 3, isoform b [Caenorhabditis elegans] ref|NP_001022992.1| CYtoKinesis defect family member (cyk-3) [Caenorhabditis elegans] 0.0
blast CAE64338 Hypothetical protein CBG09021 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010460 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase N terminus 3.1E-65
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.1E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.82
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 8.8E-99
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 8.8E-99
Sequence
Target Sequence 
>C. ELEGANS:194075_S_AT
ggatgtccaatccgtccagatgagagcaaagtattcattcctgccaactgtccgattgca
gtggattggcttccaatcgctctctaccttcgatacaatcactcgcaggagcaagcatgt
gaagatgatccttcagttgccgaaacttggtcaaggcactttgctccaagctcattggag
cactgcattgagaagttctcatgccctgagacacttgacgcagcaattcaatgtgaccgt
tgtgagaagaagacgatgagagacaaagtgatgactatttggaagcttccgaagtacttg
ataattcatctcaaacggttcgaatttctccgcgaacaaggtcgaatgggaaagtgcaaa
agaacggtgaatttcccgttgaagcacttcgatccagctccatttgttgacaagcccgac
ggaaatacatatgaatgcatcgcactcgctaatcactatggacaactatcctgtggacat
ttcatagcctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGTCCAATCCGTCCAGATGAGA 262 513 2524 Antisense
AAAGTATTCATTCCTGCCAACTGTC 261 123 2551 Antisense
GTGGATTGGCTTCCAATCGCTCTCT 261 489 2584 Antisense
ACCTTCGATACAATCACTCGCAGGA 344 87 2609 Antisense
AGATGATCCTTCAGTTGCCGAAACT 309 67 2646 Antisense
AACTTGGTCAAGGCACTTTGCTCCA 8 139 2667 Antisense
CTCATGCCCTGAGACACTTGACGCA 474 229 2721 Antisense
GAATTTCTCCGCGAACAAGGTCGAA 233 325 2845 Antisense
GAATTTCCCGTTGAAGCACTTCGAT 198 315 2892 Antisense
AGCTCCATTTGTTGACAAGCCCGAC 532 79 2919 Antisense
ATCCTGTGGACATTTCATAGCCTAT 76 39 2991 Antisense
Sequence Source Affymetrix Proprietary Database