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[194062_at] GUANYLATE CYCLASE SOLUBLE, BETA-2 CHAIN (EC 4.6.1.2) (76 KD CHAIN)

Description C46E1.2 /REP_DB=WormBase Gene ID /WP=CE19736 /GEN=gcy-36 /TR=Q9XTE0 /GB=CAB17073.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=GUANYLATE CYCLASE SOLUBLE, BETA-2 CHAIN (EC 4.6.1.2) (76 KD CHAIN)
Cluster 6grp_high1_10_10 > 01      4grp_high_8_8 > 41      6grp_total > 08      4grp_total > 46      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 117.1
(3)
P 		66.8
(-0.6)
P 		40.7
(-2.5)
A 		33.2
(-3)
A 		84
B 76.2
(3)
P 		61.5
(0.9)
P 		49.3
(-0.9)
A 		34.5
(-3)
P 		42
C 118.7
(3)
P 		77.7
(-0.8)
P 		54
(-3)
P 		53.9
(-3)
P 		65
avr 104
(3) 		68.7
(-0.3) 		48
(-2.3) 		40.5
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 120.6
(3)
P 		69.7
(-0.6)
P 		40.8
(-2.7)
A 		38.8
(-2.8)
A 		36.1
(-3)
A 		43.2
(-2.5)
P 		85
B 64.2
(2.3)
P 		46.5
(0.1)
P 		70
(3)
P 		61.3
(1.9)
P 		31
(-1.8)
A 		21
(-3)
A 		49
C 86.5
(3)
P 		24.4
(-2.7)
A 		20.8
(-3)
A 		36.7
(-1.5)
A 		54.9
(0.1)
P 		51.8
(-0.2)
P 		66
avr 90.4
(3) 		46.9
(-2) 		43.9
(-2.4) 		45.6
(-2.2) 		40.7
(-2.8) 		38.7
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 42.6
(3)
P 		14.5
(-1.5)
P 		10.6
(-2.1)
A 		4.9
(-3)
A 		38
B 24.3
(3)
P 		15.2
(-0.2)
P 		11.4
(-1.6)
A 		7.4
(-3)
P 		17
C 39.3
(3)
P 		22.3
(-0.4)
P 		18.8
(-1.1)
P 		9.1
(-3)
P 		30
avr 35.4
(3) 		17.3
(-0.8) 		13.6
(-1.6) 		7.1
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 22.2
(2)
P 		24.8
(3)
P 		8.9
(-3)
A 		9.8
(-2.7)
A 		10.9
(-2.2)
A 		10.5
(-2.4)
P 		16
B 22.4
(3)
P 		15.5
(0.2)
P 		17
(0.8)
P 		16.2
(0.5)
P 		12.6
(-1)
A 		7.8
(-3)
A 		15
C 32.1
(3)
P 		7.4
(-2.5)
A 		5.3
(-3)
A 		7.4
(-2.5)
A 		17.8
(-0.2)
P 		17.3
(-0.3)
P 		27
avr 25.6
(3) 		15.9
(-0.8) 		10.4
(-3) 		11.1
(-2.7) 		13.8
(-1.7) 		11.9
(-2.4) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194062_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17535
Sequence Type Exemplar sequence
Representative Public ID C46E1.2 NCBI
Target Description C46E1.2 /REP_DB=WormBase Gene ID /WP=CE19736 /GEN=gcy-36 /TR=Q9XTE0 /GB=CAB17073.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=GUANYLATE CYCLASE SOLUBLE, BETA-2 CHAIN (EC 4.6.1.2) (76 KD CHAIN)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078156(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078156 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-36) (gcy-36) mRNA, complete cds. 11/11 None
GENEFINDER00000006591 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:15484533:15485534:1 11/11 None
C46E1.2 ENSEMBL cdna:known chromosome:CEL140:X:15479085:15485618:1 gene:C46E1.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006575 7/11 Cross Hyb Matching Probes None
SNAP00000006576 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15479158-15485534(+) 100.0 100.0
Public Domain and Genome References
Gene Title GUANYLATE CYCLASE SOLUBLE, BETA-2 CHAIN (EC 4.6.1.2) (76 KD CHAIN)
Gene Symbol gcy-36
Ensembl C46E1.2
Entrez Gene 191657 Entrez gene
WormBase CE39007 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB17073 Hypothetical protein C46E1.2 [Caenorhabditis elegans] emb|CAD56232.2| Hypothetical protein C46E1.2 [Caenorhabditis elegans] gb|AAT73712.1| guanylate cyclase-like protein [Caenorhabditis elegans] sp|Q6DNF4|GCY36_CAEEL Soluble guanylate cyclase gcy-36 0.0
blast NP_510557 Guanylyl CYclase family member (gcy-36) [Caenorhabditis elegans] 0.0
blast CAE63319 Hypothetical protein CBG07710 [Caenorhabditis briggsae] 0.0
blast CAB17073 Hypothetical protein C46E1.2 [Caenorhabditis elegans] emb|CAD56232.2| Hypothetical protein C46E1.2 [Caenorhabditis elegans] gb|AAT73712.1| guanylate cyclase-like protein [Caenorhabditis elegans] sp|Q6DNF4|GCY36_CAEEL Soluble guanylate cyclase gcy-36 1.0E-154
blast NP_510557 Guanylyl CYclase family member (gcy-36) [Caenorhabditis elegans] 1.0E-154
blast CAE63319 Hypothetical protein CBG07710 [Caenorhabditis briggsae] 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.9E-68
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.9E-68
Sequence
Target Sequence 
>C. ELEGANS:194062_AT
ggaattcctgacttggtcgatgatcattgtgaagtgatctgccatttggccctcggaatg
gtcatggaagctcggaccgtttgtgatcccataaccaacacccctcttcatattcgtgca
ggaattcattcggggccagttgtagcaggtgttgttggcgcgaaaatgccgagatactgc
ttgttcggtgatacagtgaatacttcctcgagaatggagagtcatagtccaattggacga
attcattgttcggagaatgcaaagaagtgcgctgaaagcactgggcggtttgagtttgaa
ccacgtggcagagtacagatcaagggaaagggggagatgaatacctactttttgttgcgt
agcttcaagagatcgatttgggagatcatcgaccgcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATTCCTGACTTGGTCGATGATC 696 533 1558 Antisense
TGTGAAGTGATCTGCCATTTGGCCC 254 557 1585 Antisense
TTTGGCCCTCGGAATGGTCATGGAA 424 615 1602 Antisense
GGAAGCTCGGACCGTTTGTGATCCC 45 531 1623 Antisense
TTTGTGATCCCATAACCAACACCCC 84 667 1637 Antisense
ACCCCTCTTCATATTCGTGCAGGAA 685 89 1657 Antisense
GAATTCATTCGGGGCCAGTTGTAGC 322 325 1679 Antisense
GAAAATGCCGAGATACTGCTTGTTC 19 353 1719 Antisense
CGCTGAAAGCACTGGGCGGTTTGAG 165 255 1827 Antisense
GATGAATACCTACTTTTTGTTGCGT 452 411 1893 Antisense
GATTTGGGAGATCATCGACCGCAGA 592 427 1932 Antisense
Sequence Source Affymetrix Proprietary Database