[Home] | [Total List]

[194058_s_at]

Description C12D8.10B /REP_DB=WormBase Gene ID /WP=CE05274 /GEN=akt-1 /TR=Q17942 /GB=CAA98238.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain, Protein kinase C terminal domain
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 45      4grp_high_8_8 > 08      6grp_highest_nonorm > 79      6grp_total > 78      4grp_total > 23      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 647
1 2 3 4 range
A 1676.1
(3)
P 		1240.2
(-3)
P 		1287.7
(-2.3)
P 		1356.9
(-1.4)
P 		436
B 1841.3
(3)
P 		1194.7
(-3)
P 		1311.1
(-1.9)
P 		1808.8
(2.7)
P 		647
C 1690
(3)
P 		1203.6
(-3)
P 		1375.5
(-0.9)
P 		1604.4
(1.9)
P 		486
avr 1735.8
(3) 		1212.8
(-3) 		1324.8
(-1.7) 		1590
(1.3) 		523

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1621
1 2 3 4 5 6 range
A 1295.9
(-2.2)
P 		1597.6
(-1)
P 		1069.7
(-3)
P 		1453.9
(-1.6)
P 		1604.3
(-1)
P 		2690.6
(3)
P 		1621
B 1360.8
(-2)
P 		1420.7
(-1.7)
P 		1121.3
(-3)
P 		1291.2
(-2.3)
P 		1634.7
(-0.9)
P 		2555
(3)
P 		1434
C 1509.8
(-1.6)
P 		1469.5
(-1.8)
P 		1455.9
(-1.8)
P 		1183.5
(-3)
P 		1699.3
(-0.8)
P 		2569.7
(3)
P 		1386
avr 1388.8
(-2.3) 		1495.9
(-1.8) 		1215.6
(-3) 		1309.5
(-2.6) 		1646.1
(-1.1) 		2605.1
(3) 		1390
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 412
1 2 3 4 range
A 610.3
(3)
P 		269.2
(-2)
P 		336.6
(-1)
P 		198.6
(-3)
P 		412
B 587.2
(3)
P 		295.1
(-3)
P 		303.8
(-2.8)
P 		385.4
(-1.1)
P 		292
C 559.4
(3)
P 		346
(-1.4)
P 		479.4
(1.3)
P 		270.5
(-3)
P 		289
avr 585.6
(3) 		303.4
(-2.6) 		373.3
(-1.2) 		284.8
(-3) 		301

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 720
1 2 3 4 5 6 range
A 238.9
(-2.9)
P 		568.2
(1.8)
P 		233
(-3)
P 		368.7
(-1.1)
P 		484.1
(0.6)
P 		655.1
(3)
P 		422
B 473.5
(-1.2)
P 		472.9
(-1.2)
P 		272.3
(-3)
P 		342
(-2.4)
P 		664.1
(0.5)
P 		952.7
(3)
P 		680
C 559.6
(0.1)
P 		448.4
(-1)
P 		370.6
(-1.7)
P 		238
(-3)
P 		549.9
(0)
P 		860.2
(3)
P 		622
avr 424
(-1.5) 		496.5
(-0.7) 		292
(-3) 		316.2
(-2.7) 		566
(0.1) 		822.7
(3) 		531
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194058_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12013
Sequence Type Exemplar sequence
Representative Public ID C12D8.10B NCBI
Target Description C12D8.10B /REP_DB=WormBase Gene ID /WP=CE05274 /GEN=akt-1 /TR=Q17942 /GB=CAA98238.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain, Protein kinase C terminal domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028475(11), NM_001028474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028475 NCBI Caenorhabditis elegans AKT kinase family member (akt-1) (akt-1) mRNA, complete cds. 11/11 None
NM_001028474 NCBI Caenorhabditis elegans AKT kinase family member (akt-1) (akt-1) mRNA, complete cds. 11/11 None
SNAP00000021008 ENSEMBL cdna:SNAP chromosome:CEL140:V:10251537:10255032:1 11/11 None
GENEFINDER00000021017 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10250814:10255032:1 11/11 None
C12D8.10b.2 ENSEMBL cdna:known chromosome:CEL140:V:10251486:10255476:1 gene:C12D8.10 11/11 A 
C12D8.10b.1 ENSEMBL cdna:known chromosome:CEL140:V:10251488:10255476:1 gene:C12D8.10 11/11 A 
C12D8.10a ENSEMBL cdna:known chromosome:CEL140:V:10251488:10255476:1 gene:C12D8.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179424 3 2 NM_001028474 175808_s_at (11), 194058_s_at (11)
NM_001028475 175808_s_at (11), 194058_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10251536-10255032(+) 100.0 100.0
Public Domain and Genome References
Ensembl C12D8.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98238 Hypothetical protein C12D8.10b [Caenorhabditis elegans] ref|NP_001023646.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62467.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAA98240 Hypothetical protein C12D8.10a [Caenorhabditis elegans] ref|NP_001023645.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62466.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAE64774 Hypothetical protein CBG09565 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-69
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-60
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 6.0E-99
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 1.0E-106
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-94
Pfam IPR001849 EMBL-EBI Pleckstrin-like 4.9E-23
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Pfam IPR000719 EMBL-EBI Protein kinase 3.1E-93
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Pfam IPR000719 EMBL-EBI Protein kinase 4.8E-41
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-68
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Pfam IPR000719 EMBL-EBI Protein kinase 4.8E-41
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-68
Pfam IPR001849 EMBL-EBI Pleckstrin-like 4.9E-23
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Sequence
Target Sequence 
>C. ELEGANS:194058_S_AT
aactccggagtatcttgcaccagaggttcttgatgatcatgactacggacgttgtgttga
ttggtggggagttggagttgtgatgtacgagatgatgtgtggaaggcttccattttattc
gaaggatcataataaactattcgaacttatcatggctggtgatctaaggttcccaagcaa
gttgagtcaagaagctagaactttgctcaccggacttcttgtcaaagatccaactcagcg
attgggtggtggacccgaagatgcattggagatttgtagagcagactttttccgtactgt
cgattgggaagcaacgtacagaaaagaaatcgagccaccatataagccaaatgtgcagtc
agaaactgatactagttactttgataatgaattcacatctcaaccagttcaactgacacc
gcctagtagaagcggagcccttgctacagtcgatgagcaagaagaaatgcaatcaaactt
cacacaattctcattccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTCCGGAGTATCTTGCACCAGAG 352 137 1086 Antisense
GATGATCATGACTACGGACGTTGTG 509 409 1117 Antisense
GGCTGGTGATCTAAGGTTCCCAAGC 42 541 1239 Antisense
GAAGCTAGAACTTTGCTCACCGGAC 79 343 1276 Antisense
GGTGGTGGACCCGAAGATGCATTGG 44 501 1330 Antisense
AGAGCAGACTTTTTCCGTACTGTCG 421 69 1363 Antisense
GAAATCGAGCCACCATATAAGCCAA 425 361 1411 Antisense
GAATTCACATCTCAACCAGTTCAAC 321 325 1474 Antisense
TCAACTGACACCGCCTAGTAGAAGC 244 631 1494 Antisense
GTAGAAGCGGAGCCCTTGCTACAGT 24 457 1511 Antisense
AAACTTCACACAATTCTCATTCCAC 213 129 1560 Antisense
Sequence Source Affymetrix Proprietary Database