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[194053_s_at] ACYL-COENZYME A OXIDASE, PEROXISOMAL (EC 1.3.3.6) (PALMITOYL-COA OXIDASE) (AOX)

Description F25C8.1 /REP_DB=WormBase Gene ID /WP=CE18634 /TR=Q9XV65 /GB=CAB04173.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=ACYL-COENZYME A OXIDASE, PEROXISOMAL (EC 1.3.3.6) (PALMITOYL-COA OXIDASE) (AOX)
Cluster 6grp_total > 48      4grp_total > 43      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 61.6
(-0.9)
P 		99.8
(3)
A 		40.7
(-3)
A 		71.3
(0.1)
A 		59
B 33.7
(-3)
A 		69.5
(3)
A 		56.8
(0.9)
A 		45
(-1.1)
A 		36
C 72
(3)
P 		59.4
(-1)
P 		53.2
(-3)
A 		57.5
(-1.6)
A 		19
avr 55.8
(-1.7) 		76.2
(3) 		50.2
(-3) 		57.9
(-1.2) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 56.3
(0.8)
A 		35.2
(-3)
A 		59.4
(1.4)
A 		66.8
(2.7)
A 		63.2
(2.1)
A 		68.2
(3)
A 		33
B 53.5
(3)
A 		51.8
(2.8)
M 		6.2
(-3)
A 		39.4
(1.2)
A 		36.7
(0.9)
A 		25.3
(-0.6)
A 		47
C 37.3
(-1.4)
A 		35
(-1.9)
A 		42.5
(-0.1)
A 		45.7
(0.7)
A 		30.7
(-3)
A 		55.1
(3)
A 		24
avr 49
(2.3) 		40.7
(-1.1) 		36
(-3) 		50.6
(3) 		43.5
(0.1) 		49.5
(2.5) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 22.4
(3)
P 		21.7
(2.7)
A 		10.6
(-2.9)
A 		10.4
(-3)
A 		12
B 10.7
(-2.1)
A 		17.2
(3)
A 		13.2
(-0.2)
A 		9.6
(-3)
A 		8
C 23.8
(3)
P 		17.1
(0.1)
P 		18.5
(0.7)
A 		9.7
(-3)
A 		14
avr 19
(3) 		18.7
(2.8) 		14.1
(-0.2) 		9.9
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 10.4
(-3)
A 		12.5
(-1.6)
A 		12.9
(-1.3)
A 		17
(1.6)
A 		19.1
(3)
A 		16.6
(1.3)
A 		9
B 18.6
(3)
A 		17.3
(2.5)
M 		1.5
(-3)
A 		10.4
(0.1)
A 		14.9
(1.7)
A 		9.4
(-0.2)
A 		17
C 13.8
(0)
A 		10.7
(-2)
A 		10.8
(-2)
A 		9.2
(-3)
A 		9.9
(-2.5)
A 		18.4
(3)
A 		9
avr 14.3
(2.5) 		13.5
(1.8) 		8.4
(-3) 		12.2
(0.6) 		14.6
(2.8) 		14.8
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194053_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13029
Sequence Type Exemplar sequence
Representative Public ID F25C8.1 NCBI
Target Description F25C8.1 /REP_DB=WormBase Gene ID /WP=CE18634 /TR=Q9XV65 /GB=CAB04173.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=ACYL-COENZYME A OXIDASE, PEROXISOMAL (EC 1.3.3.6) (PALMITOYL-COA OXIDASE) (AOX)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075635(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075635 NCBI Caenorhabditis elegans F25C8.1 (F25C8.1) mRNA, complete cds. 11/11 None
SNAP00000010152 ENSEMBL cdna:SNAP chromosome:CEL140:V:20905521:20907797:-1 11/11 None
GENEFINDER00000010157 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20905521:20907519:-1 9/11 None
F25C8.1 ENSEMBL cdna:known chromosome:CEL140:V:20905472:20907797:-1 gene:F25C8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20905520-20907797(-) 100.0 100.0
Public Domain and Genome References
Gene Title ACYL-COENZYME A OXIDASE, PEROXISOMAL (EC 1.3.3.6) (PALMITOYL-COA OXIDASE) (AOX)
Ensembl F25C8.1
Entrez Gene 180376 Entrez gene
SwissProt Q9XV65 EMBL-EBI
WormBase CE18634 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6635 fatty acid beta-oxidation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5777 peroxisome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3997 acyl-CoA oxidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04173 Hypothetical protein F25C8.1 [Caenorhabditis elegans] ref|NP_508036.1| F25C8.1 [Caenorhabditis elegans] 0.0
blast CAE61624 Hypothetical protein CBG05550 [Caenorhabditis briggsae] 1.0E-162
blast CAE69737 Hypothetical protein CBG16008 [Caenorhabditis briggsae] 1.0E-143
Protein Families
Method ID Description E-Value
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-69
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR002655 EMBL-EBI Acyl-CoA oxidase 5.9E-45
Pfam IPR002655 EMBL-EBI Acyl-CoA oxidase 3.8E-41
Sequence
Target Sequence 
>C. ELEGANS:194053_S_AT
gcagtgtcgactagcatgcggtggccacggttattctcacgctagtggtattccagagct
gtccgcgttttcttgtggcagctgtacttatgaaggagacaacatagtgttgctattgca
agtggcaaacgaatgcgaactctacccagaacatgaagcttggaatcgttgctctatcga
gctatgcaaggctgcaaggtggcacgtccgactttatatagtacggaattttcttcaaaa
agtttgcacagctccaaaggatctgcaaccagttcttcgagcgctttcaaatctgtatat
atttgatcttcaagtatctaataaaggtcattttatggaaaacggatatatgacatctca
acagatagaccaactcaaaatgggtattaacgagtcactttccacgattcgtccggatgc
tgtttccattgtggacggttttgctatacacgaatttgaactcaaaagtgttctcggtcg
acga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGTGTCGACTAGCATGCGGTGGC 691 311 1209 Antisense
GCCACGGTTATTCTCACGCTAGTGG 571 279 1232 Antisense
CTAGTGGTATTCCAGAGCTGTCCGC 364 225 1250 Antisense
CGAATGCGAACTCTACCCAGAACAT 570 253 1338 Antisense
GCTTGGAATCGTTGCTCTATCGAGC 46 307 1366 Antisense
CAAGGTGGCACGTCCGACTTTATAT 295 183 1403 Antisense
AAGGATCTGCAACCAGTTCTTCGAG 37 161 1465 Antisense
GTTCTTCGAGCGCTTTCAAATCTGT 353 443 1480 Antisense
TCACTTTCCACGATTCGTCCGGATG 41 617 1603 Antisense
TCCGGATGCTGTTTCCATTGTGGAC 47 595 1620 Antisense
ACTCAAAAGTGTTCTCGGTCGACGA 591 99 1668 Antisense
Sequence Source Affymetrix Proprietary Database