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[194043_at]

Description F35D2.5 /REP_DB=WormBase Gene ID /WP=CE02727 /TR=Q20047 /GB=AAA68329.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=transcriptional activator HAP4 and regulatory domain of BCR and P85A
Cluster 6grp_total > 15      4grp_total > 46      6grp_cgc > 23      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 451
1 2 3 4 range
A 705.8
(3)
P 		546.2
(0.2)
M 		367.6
(-3)
A 		385.1
(-2.7)
P 		338
B 728.2
(3)
P 		423
(-1.1)
P 		276.8
(-3)
A 		308.4
(-2.6)
P 		451
C 508
(3)
P 		441.4
(1)
A 		307.7
(-3)
A 		397.8
(-0.3)
M 		200
avr 647.3
(3) 		470.2
(-0.2) 		317.4
(-3) 		363.8
(-2.2) 		330

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 826
1 2 3 4 5 6 range
A 992.3
(3)
P 		767.7
(1.2)
P 		325.5
(-2.3)
P 		335.6
(-2.3)
A 		431.6
(-1.5)
M 		244.1
(-3)
A 		748
B 795.2
(3)
P 		640.8
(1.5)
P 		260.5
(-2.1)
A 		360
(-1.2)
M 		312.2
(-1.6)
A 		166.1
(-3)
A 		629
C 706.9
(3)
P 		653
(2.4)
P 		389.4
(-0.5)
P 		395.6
(-0.5)
A 		207.7
(-2.6)
A 		168.3
(-3)
A 		539
avr 831.5
(3) 		687.2
(1.6) 		325.1
(-1.8) 		363.7
(-1.4) 		317.2
(-1.8) 		192.8
(-3) 		639
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 257
(3)
P 		118.6
(-1.1)
M 		96.1
(-1.8)
A 		56.4
(-3)
P 		201
B 232.2
(3)
P 		104.5
(-1.6)
P 		64.1
(-3)
A 		65.7
(-2.9)
P 		168
C 168.2
(3)
P 		126.9
(0.5)
A 		107.3
(-0.6)
A 		67.1
(-3)
M 		101
avr 219.1
(3) 		116.7
(-0.9) 		89.2
(-2) 		63.1
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 182.9
(0.5)
P 		273
(3)
P 		70.9
(-2.7)
P 		85.1
(-2.3)
A 		130.2
(-1)
M 		59.4
(-3)
A 		214
B 276.7
(3)
P 		213.3
(1.2)
P 		63.2
(-3)
A 		95.4
(-2.1)
M 		126.9
(-1.2)
A 		61.9
(-3)
A 		215
C 262
(3)
P 		199.2
(1.2)
P 		99.1
(-1.8)
P 		79.6
(-2.3)
A 		67.2
(-2.7)
A 		56.3
(-3)
A 		206
avr 240.5
(3) 		228.5
(2.6) 		77.7
(-2.4) 		86.7
(-2.1) 		108.1
(-1.4) 		59.2
(-3) 		181
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 194043_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5769
Sequence Type Exemplar sequence
Representative Public ID F35D2.5 NCBI
Target Description F35D2.5 /REP_DB=WormBase Gene ID /WP=CE02727 /TR=Q20047 /GB=AAA68329.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=transcriptional activator HAP4 and regulatory domain of BCR and P85A
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063191 NCBI Caenorhabditis elegans SYnapse Defective family member (syd-1) (syd-1) mRNA, complete cds. 11/11 A 
F35D2.5b ENSEMBL cdna:known chromosome:CEL140:II:7585691:7591946:-1 gene:F35D2.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026977 8/11 Cross Hyb Matching Probes None
NM_001026975 8/11 Cross Hyb Matching Probes None
F35D2.5c.2 8/11 Cross Hyb Matching Probes None
F35D2.5c.1 8/11 Cross Hyb Matching Probes None
F35D2.5a 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174230 3 1 NM_001026977 174665_at (11)
NM_063191 174665_at (11), 194043_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7585998-7594839(-) 100.0 100.0
Public Domain and Genome References
Ensembl F35D2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO21428 Synapse defective protein 1, isoform b [Caenorhabditis elegans] ref|NP_495592.2| SYnapse Defective family member (syd-1) [Caenorhabditis elegans] gb|AAN38752.1| axon identity specification protein SYD-1 [Caenorhabditis elegans] 0.0
blast T16275 hypothetical protein F35D2.5 - Caenorhabditis elegans 0.0
blast AAO38644 Synapse defective protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022146.1| SYnapse Defective family member (syd-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.8E-7
Pfam IPR000008 EMBL-EBI C2 domain 0.0020
Pfam IPR000198 EMBL-EBI RhoGAP domain 8.2E-28
Sequence
Target Sequence 
>C. ELEGANS:194043_AT
agctgcttcagttgcactaccaaatgatgttcaaacgaatcgacatctcgtcttaaaaat
cattgactgccttcaactctctgctaaaaactgtctacttctagttcttgatcatttgag
tacagttctatgctcttctccacataatgggctgactccgactcgattatcattgatatt
tgcaccacttcttttcttttgtctcgacacgttctctccatatacaatatcaccaacatc
caaaatggcagcagtccgtacactcgatatcaatcaagcatcttcaagtctccaaatgat
tttgtcgatatggccctcaagagttagtgagttggtcgagttctttccttttctactcaa
ctatcctacagtatcatacagtagtccgaatccacacagcgaaagcggatcagatagtcc
ggccacgacatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTGCTTCAGTTGCACTACCAAAT 466 81 2673 Antisense
TGCCTTCAACTCTCTGCTAAAAACT 583 585 2740 Antisense
TGAGTACAGTTCTATGCTCTTCTCC 175 569 2789 Antisense
GCTGACTCCGACTCGATTATCATTG 7 297 2823 Antisense
TTGATATTTGCACCACTTCTTTTCT 342 705 2845 Antisense
ACACGTTCTCTCCATATACAATATC 191 111 2879 Antisense
GCAGTCCGTACACTCGATATCAATC 523 311 2923 Antisense
GTGAGTTGGTCGAGTTCTTTCCTTT 295 479 2999 Antisense
CCTTTTCTACTCAACTATCCTACAG 397 269 3019 Antisense
ATACAGTAGTCCGAATCCACACAGC 591 23 3048 Antisense
ATCAGATAGTCCGGCCACGACATGT 444 27 3081 Antisense
Sequence Source Affymetrix Proprietary Database