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[194042_s_at] origin recognition complex, second largest subunit ORC2

Description F59E10.1 /REP_DB=WormBase Gene ID /WP=CE11492 /GEN=orc-2 /TR=SW:Q21037 /GB=CAA85415.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=origin recognition complex, second largest subunit ORC2
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 87      4grp_highest_8_8 > 26      4grp_high_8_8 > 58      6grp_highest_nonorm > 81      6grp_total > 77      4grp_total > 61      6grp_highest_sub > Da      6grp_cgc > 39      6grp_high1_sub > In      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 252.9
(3)
P 		203.2
(-3)
P 		226.3
(-0.2)
P 		203.8
(-2.9)
P 		50
B 243
(2.9)
P 		223.8
(2)
P 		245.2
(3)
P 		117.8
(-3)
P 		127
C 254.8
(0.1)
P 		224.5
(-1.9)
P 		297.9
(3)
P 		207.7
(-3)
P 		90
avr 250.2
(2.5) 		217.2
(0.1) 		256.5
(3) 		176.4
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 217.5
(-2.7)
P 		239.4
(-2)
P 		205.9
(-3)
P 		274.9
(-1)
P 		246.8
(-1.8)
P 		411.6
(3)
P 		206
B 191.8
(-2.4)
P 		203.3
(-1.9)
P 		178.4
(-3)
P 		295
(2.3)
P 		252.8
(0.4)
P 		310.4
(3)
P 		132
C 310.6
(3)
P 		193.7
(-3)
P 		251
(-0.1)
P 		203
(-2.5)
P 		274
(1.1)
P 		286
(1.7)
P 		117
avr 240
(-1.6) 		212.1
(-3) 		211.8
(-3) 		257.6
(-0.8) 		257.9
(-0.8) 		336
(3) 		124
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 92.1
(3)
P 		44.1
(-1.6)
P 		59.2
(-0.2)
P 		29.8
(-3)
P 		62
B 77.5
(3)
P 		55.3
(0.5)
P 		56.8
(0.6)
P 		25.1
(-3)
P 		52
C 84.3
(1.3)
P 		64.5
(-0.4)
P 		103.8
(3)
P 		35
(-3)
P 		69
avr 84.6
(3) 		54.6
(-0.3) 		73.3
(1.8) 		30
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 40.1
(-3)
P 		85.1
(1.5)
P 		44.8
(-2.5)
P 		69.7
(-0)
P 		74.5
(0.4)
P 		100.2
(3)
P 		60
B 66.7
(-1.1)
P 		67.7
(-1)
P 		43.3
(-3)
P 		78.1
(-0.1)
P 		102.7
(1.9)
P 		115.7
(3)
P 		72
C 115.1
(3)
P 		59.1
(-1.5)
P 		63.9
(-1.1)
P 		40.8
(-3)
P 		88.7
(0.9)
P 		95.7
(1.4)
P 		74
avr 74
(-0.4) 		70.6
(-0.8) 		50.7
(-3) 		62.9
(-1.6) 		88.6
(1.3) 		103.9
(3) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194042_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2980
Sequence Type Exemplar sequence
Representative Public ID F59E10.1 NCBI
Target Description F59E10.1 /REP_DB=WormBase Gene ID /WP=CE11492 /GEN=orc-2 /TR=SW:Q21037 /GB=CAA85415.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=origin recognition complex, second largest subunit ORC2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063938(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063938 NCBI Caenorhabditis elegans ORC family member (orc-2) (orc-2) mRNA, complete cds. 11/11 A 
SNAP00000001007 ENSEMBL cdna:SNAP chromosome:CEL140:II:10873845:10877225:1 11/11 None
GENEFINDER00000001008 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10873845:10877225:1 11/11 None
F59E10.1 ENSEMBL cdna:known chromosome:CEL140:II:10873842:10877768:1 gene:F59E10.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10873841-10877222(+) 100.0 100.0
Public Domain and Genome References
Gene Title origin recognition complex, second largest subunit ORC2
Gene Symbol orc-2
Ensembl F59E10.1
Entrez Gene 174676 Entrez gene
SwissProt Q21037 EMBL-EBI
WormBase CE11492 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17091_S_AT origin recognition complex subunit 2 (ORC2) at
ATH1-121501:267173_AT origin recognition complex subunit 2 (ORC2) at
CANINE:1604191_AT similar to origin recognition complex, subunit 2 cfa
CANINE:1591003_AT similar to origin recognition complex, subunit 2 cfa
CANINE_2:CFA.20287.1.S1_S_AT similar to origin recognition complex, subunit 2 cfa
CANINE_2:CFA.5361.1.A1_AT similar to origin recognition complex, subunit 2 cfa
DROSOPHILA_2:1635760_AT Origin recognition complex subunit 2 dm
DROSGENOME1:152530_AT Origin recognition complex subunit 2 dm
CHICKEN:GGAAFFX.12525.1.S1_S_AT origin recognition complex, subunit 2-like (yeast) gga
HU35KSUBC:RC_N66852_AT origin recognition complex, subunit 2-like (yeast) hs
HUGENEFL:U27459_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U133A:204853_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U133_PLUS_2:204853_AT origin recognition complex, subunit 2-like (yeast) hs
HG-FOCUS:204853_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U133A_2:204853_AT origin recognition complex, subunit 2-like (yeast) hs
U133_X3P:G5453829_3P_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U95AV2:39943_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U95B:45023_R_AT origin recognition complex, subunit 2-like (yeast) hs
HG-U95E:90865_AT Origin recognition complex, subunit 2-like (yeast) hs
MU11KSUBA:C80251_RC_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MU11KSUBA:U27457_S_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MG-U74AV2:160838_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOE430A:1418225_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430A_2:1418225_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430_2:1418225_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOE430A:1418226_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430A_2:1418226_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430_2:1418226_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOE430A:1418227_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430A_2:1418227_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430_2:1418227_AT origin recognition complex, subunit 2-like (S. cerevisiae) mm
MOUSE430_2:1443800_AT Origin recognition complex, subunit 2-like (S. cerevisiae), mRNA (cDNA clone MGC:18406 IMAGE:4234871) mm
MOE430B:1443800_AT Origin recognition complex, subunit 2-like (S. cerevisiae), mRNA (cDNA clone MGC:18406 IMAGE:4234871) mm
RG-U34C:RC_AI145141_AT Origin recognition complex, subunit 2-like (S. cerevisiae) (predicted) rn
RAE230A:1389141_AT Origin recognition complex, subunit 2-like (S. cerevisiae) (predicted) rn
RAT230_2:1389141_AT Origin recognition complex, subunit 2-like (S. cerevisiae) (predicted) rn
YEAST_2:1773725_AT Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6260 DNA replication inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
808 origin recognition complex inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85415 Hypothetical protein F59E10.1 [Caenorhabditis elegans] ref|NP_496339.1| ORC family member (orc-2) [Caenorhabditis elegans] sp|Q21037|ORC2_CAEEL Origin recognition complex subunit 2 (CeOrc2) gb|AAC46954.1| ceOrc2p 0.0
blast CAE59591 Hypothetical protein CBG02998 [Caenorhabditis briggsae] 1.0E-165
blast AAF66068 origin recognition complex subunit 2 [Cricetulus griseus] 8.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR007220 EMBL-EBI Origin recognition complex subunit 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:194042_S_AT
agttgggctcgatctattcgcagaaaaatgaatagccaacagttgattcttatcattgat
aatattgaagctcctgattggagaagtgatcaagaagcattttgcgaacttcttgagaat
cgggattcggtgaaattgattgctacagttgatcacatttactcgacgttcatctggaat
tcgcgtcaactatcatcactctcattcgttcacatcacaatcaacaccttcgaaattcca
cttcaagaattaatgactggagattctcgtcttcttggtcttgatgctcgttcgaatcaa
tcctctcatacaatgtcatcgcttgatgtgttctggaaatctcttgccgtcaattcacaa
aaattattccgtctctttttccaaatgtactttgacaccaagaagcctgtcaaattctgg
gatttgttcaatgcggcaaaagatgatttcattgcttcaactgacgctgctcttcgaacc
caacttgtcgaattcaaggatcatcgggttttgaagtggacccgtggtgatgacggaaac
gatcagctgtcgggcattgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTGGGCTCGATCTATTCGCAGAA 342 65 676 Antisense
GATCACATTTACTCGACGTTCATCT 615 421 826 Antisense
GGAATTCGCGTCAACTATCATCACT 539 533 851 Antisense
AATCAACACCTTCGAAATTCCACTT 110 171 894 Antisense
GACTGGAGATTCTCGTCTTCTTGGT 49 377 930 Antisense
TTCTTGGTCTTGATGCTCGTTCGAA 667 689 947 Antisense
TCGTTCGAATCAATCCTCTCATACA 393 601 963 Antisense
TGGAAATCTCTTGCCGTCAATTCAC 450 547 1009 Antisense
TTCCGTCTCTTTTTCCAAATGTACT 135 699 1042 Antisense
TTTCATTGCTTCAACTGACGCTGCT 180 673 1122 Antisense
AACGATCAGCTGTCGGGCATTGTCG 349 143 1213 Antisense
Sequence Source Affymetrix Proprietary Database