[Home] | [Total List]

[194036_s_at] alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

Description M01F1.1 /REP_DB=WormBase Gene ID /WP=CE01029 /TR=Q21450 /GB=CAA86513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Cluster 6grp_total > 88      4grp_total > 10      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 3.3
(-3)
A 		6.4
(-2.5)
A 		6.3
(-2.5)
A 		40.7
(3)
A 		37
B 1.8
(-3)
A 		10.9
(0.3)
A 		3.2
(-2.5)
A 		18.6
(3)
A 		17
C 4.1
(-2.8)
A 		2.6
(-3)
A 		4.2
(-2.8)
A 		57.9
(3)
A 		55
avr 3.1
(-3) 		6.6
(-2.4) 		4.6
(-2.8) 		39.1
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 9.4
(-2.6)
A 		17.2
(-2.1)
A 		3
(-3)
A 		11.3
(-2.5)
A 		72.2
(1.3)
A 		98.5
(3)
A 		96
B 4.7
(-2.8)
A 		2.2
(-3)
A 		11.9
(-2.4)
A 		12.1
(-2.4)
A 		32.1
(-1.1)
A 		97.7
(3)
A 		96
C 6.6
(-2.9)
A 		5.6
(-3)
A 		6.2
(-3)
A 		7.9
(-2.9)
A 		60.1
(0.1)
A 		110
(3)
P 		104
avr 6.9
(-3) 		8.3
(-2.9) 		7
(-3) 		10.4
(-2.8) 		54.8
(0) 		102.1
(3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.2
(-3)
A 		1.4
(-2.8)
A 		1.6
(-2.5)
A 		6
(3)
A 		5
B 0.6
(-3)
A 		2.7
(0.7)
A 		0.7
(-2.8)
A 		4
(3)
A 		3
C 1.3
(-2.7)
A 		0.8
(-3)
A 		1.5
(-2.5)
A 		9.8
(3)
A 		9
avr 1
(-3) 		1.6
(-2.4) 		1.3
(-2.7) 		6.6
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 1.7
(-2.7)
A 		6.1
(-1.6)
A 		0.6
(-3)
A 		2.9
(-2.4)
A 		21.8
(2.4)
A 		24
(3)
A 		23
B 1.6
(-2.8)
A 		0.7
(-3)
A 		2.9
(-2.6)
A 		3.2
(-2.6)
A 		13
(-0.9)
A 		36.4
(3)
A 		36
C 2.4
(-2.9)
A 		1.7
(-3)
A 		1.6
(-3)
A 		1.6
(-3)
A 		19.4
(0)
A 		36.8
(3)
P 		35
avr 1.9
(-3) 		2.8
(-2.8) 		1.7
(-3) 		2.6
(-2.8) 		18.1
(0.2) 		32.4
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194036_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7062
Sequence Type Exemplar sequence
Representative Public ID M01F1.1 NCBI
Target Description M01F1.1 /REP_DB=WormBase Gene ID /WP=CE01029 /TR=Q21450 /GB=CAA86513.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065318(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065318 NCBI Caenorhabditis elegans GLYcosylation related family member (gly-14) (gly-14) mRNA, complete cds. 11/11 A 
SNAP00000008224 ENSEMBL cdna:SNAP chromosome:CEL140:III:3490402:3499039:-1 11/11 None
GENEFINDER00000008238 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3490402:3499039:-1 11/11 None
M01F1.1 ENSEMBL cdna:known chromosome:CEL140:III:3490402:3493215:-1 gene:M01F1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3490401-3493207(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Gene Symbol gly-14
Ensembl M01F1.1
Entrez Gene 175453 Entrez gene
SwissProt Q21450 EMBL-EBI
WormBase CE01029 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17209_S_AT alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, putative at
ATH1-121501:253035_AT alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, putative at
CANINE_2:CFA.18900.1.S1_S_AT similar to Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I) cfa
CANINE_2:CFAAFFX.20036.1.S1_AT similar to Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I) cfa
DROSOPHILA_2:1623607_AT UDP-GlcNAc:a-3-D-mannoside-1,2-N-acetylglucosaminyltransferase I dm
DROSGENOME1:153109_AT UDP-GlcNAc:a-3-D-mannoside-1,2-N-acetylglucosaminyltransferase I dm
HG-U133_PLUS_2:201126_S_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U133A_2:201126_S_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U133A:201126_S_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-FOCUS:201126_S_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HUGENEFL:M55621_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
U133_X3P:G6031182_3P_A_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U95AV2:39778_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U95C:54213_R_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U95D:76283_AT mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
HG-U95D:76285_S_AT Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase hs
MU11KSUBA:L07037_S_AT mannoside acetylglucosaminyltransferase 1 mm
MG-U74AV2:96087_AT mannoside acetylglucosaminyltransferase 1 mm
MG-U74AV2:161492_I_AT mannoside acetylglucosaminyltransferase 1 mm
MOE430A:1423609_A_AT mannoside acetylglucosaminyltransferase 1 mm
MOUSE430A_2:1423609_A_AT mannoside acetylglucosaminyltransferase 1 mm
MOUSE430_2:1423609_A_AT mannoside acetylglucosaminyltransferase 1 mm
RG-U34A:E07296CDS_S_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RG-U34A:D16302_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RG-U34A:RC_AA957132_S_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RAE230A:1367796_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RAT230_2:1367796_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RAE230B:1379088_X_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
RAT230_2:1379088_X_AT Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
139 Golgi membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3827 alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86513 Hypothetical protein M01F1.1 [Caenorhabditis elegans] ref|NP_497719.1| GLYcosylation related family member (gly-14) [Caenorhabditis elegans] gb|AAD03024.1| UDP-N-acetylglucosamine:a-3-D-mannoside b-1,2-N-acetylglucosaminyltransferase I [Caenorhabditis elegans] 0.0
blast CAE64951 Hypothetical protein CBG09782 [Caenorhabditis briggsae] 1.0E-159
blast AAG38885 Glycosylation related protein 13 [Caenorhabditis elegans] ref|NP_509566.1| GLYcosylation related family member (gly-13) [Caenorhabditis elegans] sp|Q11068|MGAT1_CAEEL Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I) gb|AAD03022.1| UDP-N-acetylglucosamine:a-3-D-mannoside b-1,2-N-acetylglucosaminyltransferase I [Caenorhabditis elegans] 1.0E-140
blast CAE64952 Hypothetical protein CBG09783 [Caenorhabditis briggsae] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.9E-10
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 2.5E-39
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497719.1 1 7-26
M01F1.1 1 7-26
GENEFINDER00000008238 2 365-387,475-497
Sequence
Target Sequence 
>C. ELEGANS:194036_S_AT
cagaagtgacttttttgccggattgggttggatgatgacacgaaaaacatgggaagaact
ggagccaatttggccgaatggattctgggatgattggatgagagagccagttcagaggaa
acaacgacaatgtattaggcctgaaattagtagaactgggatgatgaaatatggaaagga
gggcaccagcaaaggccaattcttcagtgatcatctggaaaagatcaaagtcaacgattt
gccggttgatttctcgcaaattaatctcgattatttgcaaaaaaacgaattcgaatcgag
actgagcctggatatccgaaatgctgttcccgtcgatattgacgatataacttatccaga
ttggaagccagattatgaaggaatgaaagcaataatctactacactggaagaacggattt
cgtggcaaaagccgatcggctgagtttgatgcacgattttaaggctggagtccctcgaac
tgcgtacaatggaattgtgacgtgcttctacaagggaacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAAGTGACTTTTTTGCCGGATTG 313 205 750 Antisense
GAGCCAATTTGGCCGAATGGATTCT 204 385 811 Antisense
GGAGGGCACCAGCAAAGGCCAATTC 192 517 927 Antisense
AAGGCCAATTCTTCAGTGATCATCT 113 163 941 Antisense
ACGATTTGCCGGTTGATTTCTCGCA 211 93 983 Antisense
GAATCGAGACTGAGCCTGGATATCC 454 329 1042 Antisense
AATGCTGTTCCCGTCGATATTGACG 294 167 1069 Antisense
GCCGATCGGCTGAGTTTGATGCACG 527 283 1180 Antisense
CGATTTTAAGGCTGGAGTCCCTCGA 321 247 1203 Antisense
AGTCCCTCGAACTGCGTACAATGGA 101 59 1218 Antisense
GTGACGTGCTTCTACAAGGGAACCA 273 483 1246 Antisense
Sequence Source Affymetrix Proprietary Database