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[194033_s_at]

Description F17E5.1A /REP_DB=WormBase Gene ID /WP=CE27131 /GEN=lin-2 /TR=SW:P54936 /GB=CAA90760.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=MAGUK protein, calcium-calmodulin dependent protein kinase
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 13      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 90      6grp_total > 24      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 217.9
(3)
P 		128.1
(-1.8)
P 		106.7
(-3)
P 		108.6
(-2.9)
P 		111
B 169
(3)
P 		116.7
(-2)
P 		117
(-2)
P 		106.6
(-3)
P 		62
C 220
(3)
P 		132.3
(-1.9)
P 		111.8
(-3)
P 		113.5
(-2.9)
P 		108
avr 202.3
(3) 		125.7
(-2) 		111.8
(-2.9) 		109.6
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 179.6
(3)
P 		154.3
(1.5)
P 		127.1
(-0)
P 		103.7
(-1.4)
P 		108.2
(-1.1)
P 		75.5
(-3)
P 		104
B 204.1
(3)
P 		187
(2.2)
P 		123.9
(-0.6)
P 		115.5
(-1)
P 		72.9
(-2.9)
P 		69.8
(-3)
P 		134
C 163.1
(1.2)
P 		213.5
(3)
P 		160.2
(1.1)
P 		102
(-1.1)
P 		100.8
(-1.1)
P 		49.4
(-3)
P 		164
avr 182.3
(2.9) 		184.9
(3) 		137.1
(0.6) 		107.1
(-0.9) 		94
(-1.5) 		64.9
(-3) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 79.3
(3)
P 		27.8
(-1.9)
P 		27.9
(-1.9)
P 		15.9
(-3)
P 		63
B 53.9
(3)
P 		28.8
(-1.8)
P 		27.1
(-2.2)
P 		22.7
(-3)
P 		31
C 72.8
(3)
P 		38
(-0.9)
P 		39
(-0.8)
P 		19.1
(-3)
P 		54
avr 68.7
(3) 		31.5
(-1.5) 		31.3
(-1.5) 		19.2
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 33.1
(-0.6)
P 		54.9
(3)
P 		27.7
(-1.5)
P 		26.3
(-1.7)
P 		32.7
(-0.6)
P 		18.4
(-3)
P 		37
B 71
(3)
P 		62.2
(1.8)
P 		30.1
(-2.5)
P 		30.6
(-2.4)
P 		29.6
(-2.5)
P 		26
(-3)
P 		45
C 60.5
(2.4)
P 		65.1
(3)
P 		40.8
(0)
P 		20.5
(-2.5)
P 		32.6
(-1)
P 		16.5
(-3)
P 		49
avr 54.9
(2.1) 		60.7
(3) 		32.9
(-1.1) 		25.8
(-2.2) 		31.6
(-1.3) 		20.3
(-3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194033_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17084
Sequence Type Exemplar sequence
Representative Public ID F17E5.1A NCBI
Target Description F17E5.1A /REP_DB=WormBase Gene ID /WP=CE27131 /GEN=lin-2 /TR=SW:P54936 /GB=CAA90760.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=MAGUK protein, calcium-calmodulin dependent protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029416(11), NM_001029417(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029416 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-2) (lin-2) mRNA, complete cds. 11/11 None
NM_001029417 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-2) (lin-2) mRNA, complete cds. 11/11 None
SNAP00000016293 ENSEMBL cdna:SNAP chromosome:CEL140:X:12399909:12402085:-1 11/11 None
GENEFINDER00000016297 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12399909:12405782:-1 11/11 None
F17E5.1a ENSEMBL cdna:known chromosome:CEL140:X:12399439:12413946:-1 gene:F17E5.1 11/11 A 
F17E5.1b ENSEMBL cdna:known chromosome:CEL140:X:12399439:12405782:-1 gene:F17E5.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12399908-12413946(-) 100.0 100.0
Public Domain and Genome References
Ensembl F17E5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90760 Hypothetical protein F17E5.1a [Caenorhabditis elegans] emb|CAA63314.1| LIN-2A [Caenorhabditis elegans] ref|NP_001024587.1| abnormal cell LINeage family member (lin-2) [Caenorhabditis elegans] sp|P54936|LIN2_CAEEL Abnormal cell lineage protein 2 (Protein lin-2) 0.0
blast T21073 hypothetical protein F17E5.1a - Caenorhabditis elegans 0.0
blast CAE57253 Hypothetical protein CBG00133 [Caenorhabditis briggsae] 0.0
blast CAA90759 Hypothetical protein F17E5.1b [Caenorhabditis elegans] emb|CAA63315.1| LIN-2B [Caenorhabditis elegans] ref|NP_001024588.1| abnormal cell LINeage family member (lin-2) [Caenorhabditis elegans] 0.0
blast T21072 hypothetical protein F17E5.1b - Caenorhabditis elegans 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 1.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.7E-22
Pfam IPR001452 EMBL-EBI SH3 6.8E-5
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-52
Pfam IPR004172 EMBL-EBI L27 domain 3.4E-12
Pfam IPR004172 EMBL-EBI L27 domain 3.8E-16
Pfam IPR008144 EMBL-EBI Guanylate kinase 2.6E-62
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.7E-22
Pfam IPR001452 EMBL-EBI SH3 6.8E-5
Pfam IPR004172 EMBL-EBI L27 domain 3.8E-16
Pfam IPR008144 EMBL-EBI Guanylate kinase 2.6E-62
Pfam IPR001452 EMBL-EBI SH3 6.8E-5
Pfam IPR008144 EMBL-EBI Guanylate kinase 2.6E-62
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.7E-22
Pfam IPR001452 EMBL-EBI SH3 6.8E-5
Pfam IPR004172 EMBL-EBI L27 domain 3.8E-16
Pfam IPR008144 EMBL-EBI Guanylate kinase 2.6E-62
Sequence
Target Sequence 
>C. ELEGANS:194033_S_AT
gatcaacttgatctggtgacttatgaagaagtaatgaggctgtcacaatatcggagaaaa
accttagtacttcttggagctcatggcgttggcagaagacatatcaagaacactttgata
cacagacacccgaatagatttgcatatccaattccccatacaactcgaccgccacgaaaa
gatgaagttgatggtaaacattactattttgttacaaatgagcaaatgatggcggacatt
caaaataatgaatatctagagtatggaacgcacgaagaaagtatgtatggaacaaagcta
gaaaccattcgcaatattcataagagtggaaaaattgcaattttggatgtggaaccacaa
gcattaaaggttctgaggactgcagaatattcaccatttgttgtgtttattgcagcacca
aacttgcaaggaatgcaggatccggatggttctttggaaaagctcttgaatgaatcggat
gttcttcggcaagcttttggtcaccttttcgactttatcatcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAACTTGATCTGGTGACTTATG 9 423 2290 Antisense
AACCTTAGTACTTCTTGGAGCTCAT 678 147 2349 Antisense
GGAGCTCATGGCGTTGGCAGAAGAC 124 521 2365 Antisense
GAACACTTTGATACACAGACACCCG 76 353 2397 Antisense
AGATTTGCATATCCAATTCCCCATA 539 65 2425 Antisense
ATACAACTCGACCGCCACGAAAAGA 437 1 2447 Antisense
GAGGACTGCAGAATATTCACCATTT 190 405 2664 Antisense
GTGTTTATTGCAGCACCAAACTTGC 469 485 2692 Antisense
GAATGCAGGATCCGGATGGTTCTTT 541 327 2720 Antisense
GATGTTCTTCGGCAAGCTTTTGGTC 54 411 2767 Antisense
TCACCTTTTCGACTTTATCATCACA 123 627 2790 Antisense
Sequence Source Affymetrix Proprietary Database