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[194022_s_at] MICROFIBRILLAR-ASSOCIATED PROTEIN 1 (ASSOCIATED MICROFIBRIL PROTEIN) (AMF)

Description F43G9.10 /REP_DB=WormBase Gene ID /WP=CE10372 /TR=Q93712 /GB=CAB02103.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=MICROFIBRILLAR-ASSOCIATED PROTEIN 1 (ASSOCIATED MICROFIBRIL PROTEIN) (AMF)
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 66      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 84      6grp_total > 67      4grp_total > 23      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 370
1 2 3 4 range
A 736.4
(2.3)
P 		556.4
(-1.9)
P 		510.3
(-3)
P 		766.3
(3)
P 		256
B 763.4
(3)
P 		486
(-1.5)
P 		396
(-3)
P 		651.5
(1.2)
P 		367
C 685.3
(3)
P 		482.5
(-3)
P 		544.7
(-1.2)
P 		649.4
(1.9)
P 		203
avr 728.4
(3) 		508.3
(-2.4) 		483.7
(-3) 		689.1
(2) 		245

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 448
1 2 3 4 5 6 range
A 707.1
(0.2)
P 		559.3
(-3)
P 		797.3
(2.1)
P 		622.4
(-1.6)
P 		599.7
(-2.1)
P 		837.2
(3)
P 		278
B 640.8
(-0.7)
P 		529.3
(-3)
P 		670.9
(-0.1)
P 		609.1
(-1.4)
P 		541.9
(-2.7)
P 		825.2
(3)
P 		296
C 600.5
(-2.3)
P 		683.6
(-1.1)
P 		600.8
(-2.2)
P 		626.8
(-1.9)
P 		547
(-3)
P 		977.2
(3)
P 		430
avr 649.5
(-1.4) 		590.7
(-2.5) 		689.7
(-0.6) 		619.4
(-1.9) 		562.9
(-3) 		879.9
(3) 		317
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 268.1
(3)
P 		120.8
(-2.7)
P 		133.4
(-2.2)
P 		112.2
(-3)
P 		156
B 243.4
(3)
P 		120
(-1.9)
P 		91.8
(-3)
P 		138.8
(-1.1)
P 		152
C 226.8
(3)
P 		138.7
(-1.5)
P 		189.9
(1.1)
P 		109.5
(-3)
P 		117
avr 246.1
(3) 		126.5
(-2.7) 		138.4
(-2.1) 		120.2
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 130.3
(-3)
P 		198.9
(2.6)
P 		173.6
(0.5)
P 		157.9
(-0.7)
P 		181
(1.1)
P 		203.8
(3)
P 		74
B 223
(-0.5)
P 		176.2
(-2.4)
P 		162.9
(-2.9)
P 		161.3
(-3)
P 		220.2
(-0.6)
P 		307.7
(3)
P 		146
C 222.6
(-0.1)
P 		208.6
(-0.5)
P 		152.9
(-2.2)
P 		126.1
(-3)
P 		177
(-1.5)
P 		327.1
(3)
P 		201
avr 192
(-1) 		194.6
(-0.9) 		163.1
(-2.3) 		148.4
(-3) 		192.7
(-1) 		279.5
(3) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194022_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1057
Sequence Type Exemplar sequence
Representative Public ID F43G9.10 NCBI
Target Description F43G9.10 /REP_DB=WormBase Gene ID /WP=CE10372 /TR=Q93712 /GB=CAB02103.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=MICROFIBRILLAR-ASSOCIATED PROTEIN 1 (ASSOCIATED MICROFIBRIL PROTEIN) (AMF)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059939(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059939 NCBI Caenorhabditis elegans F43G9.10 (F43G9.10) mRNA, complete cds. 11/11 None
SNAP00000043544 ENSEMBL cdna:SNAP chromosome:CEL140:I:8646723:8648360:-1 11/11 None
GENEFINDER00000043551 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8646723:8648360:-1 11/11 None
F43G9.10 ENSEMBL cdna:known chromosome:CEL140:I:8646647:8648361:-1 gene:F43G9.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8646653-8648291(-) 81.8 100.0
Public Domain and Genome References
Gene Title MICROFIBRILLAR-ASSOCIATED PROTEIN 1 (ASSOCIATED MICROFIBRIL PROTEIN) (AMF)
Ensembl F43G9.10
Entrez Gene 172661 Entrez gene
SwissProt Q93712 EMBL-EBI
WormBase CE10372 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:255093_S_AT microfibrillar-associated protein-related at
ATGENOME1:13042_AT microfibrillar-associated protein-related at
CANINE:1588826_AT similar to microfibrillar-associated protein 1 cfa
CANINE_2:CFA.11583.2.A1_S_AT similar to microfibrillar-associated protein 1 cfa
CANINE_2:CFAAFFX.20742.1.S1_S_AT similar to microfibrillar-associated protein 1 cfa
DROSOPHILA_2:1641198_AT dm
DROSGENOME1:152124_AT dm
HG-U133A:203406_AT microfibrillar-associated protein 1 hs
HG-U133_PLUS_2:203406_AT microfibrillar-associated protein 1 hs
HG-FOCUS:203406_AT microfibrillar-associated protein 1 hs
HG-U133A_2:203406_AT microfibrillar-associated protein 1 hs
HU35KSUBC:RC_AA365708_S_AT microfibrillar-associated protein 1 hs
HUGENEFL:U04209_AT microfibrillar-associated protein 1 hs
U133_X3P:G5174552_3P_AT microfibrillar-associated protein 1 hs
HG-U95AV2:36535_AT microfibrillar-associated protein 1 hs
MU11KSUBA:AA267744_F_AT microfibrillar-associated protein 1 mm
MOE430A:1449444_A_AT microfibrillar-associated protein 1 mm
MOUSE430A_2:1449444_A_AT microfibrillar-associated protein 1 mm
MOUSE430_2:1449444_A_AT microfibrillar-associated protein 1 mm
MOE430A:1449445_X_AT microfibrillar-associated protein 1 mm
MOUSE430A_2:1449445_X_AT microfibrillar-associated protein 1 mm
MOUSE430_2:1449445_X_AT microfibrillar-associated protein 1 mm
MOE430A:1419370_A_AT microfibrillar-associated protein 1 mm
MOUSE430A_2:1419370_A_AT microfibrillar-associated protein 1 mm
MOUSE430_2:1419370_A_AT microfibrillar-associated protein 1 mm
MU19KSUBA:TC20657_AT 10, 11 days embryo whole body cDNA, RIKEN full-length enriched library, clone:2810008G12 product:microfibrillar-associated protein 1, full insert sequence mm
MU19KSUBA:TC22905_AT Microfibrillar-associated protein 1 (Mfap1), mRNA mm
MU19KSUBC:TC37328_AT Microfibrillar-associated protein 1 (Mfap1), mRNA mm
MU19KSUBC:TC41258_S_AT Microfibrillar-associated protein 1 (Mfap1), mRNA mm
RAE230B:1390808_AT similar to microfibrillar-associated protein 1 rn
RAT230_2:1390808_AT similar to microfibrillar-associated protein 1 rn
Protein Similarities
Method ID Description E-Value
blast CAB02103 Hypothetical protein F43G9.10 [Caenorhabditis elegans] ref|NP_492340.1| F43G9.10 [Caenorhabditis elegans] 1.0E-142
blast CAE67086 Hypothetical protein CBG12497 [Caenorhabditis briggsae] 1.0E-104
blast XP_395869 PREDICTED: similar to ENSANGP00000028298 [Apis mellifera] 2.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR009730 EMBL-EBI Micro-fibrillar-associated 1, C-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:194022_S_AT
gatcgtgttgatttgtcatctgttctaaccgacgatgaaactgaaaatatggcatatgag
gcgtggaagctcagagaaatgaaacgattgaagagaaaccgtgacgaacgtgaagaagcc
gcacgcgagaaggcagaacttgacaagattcacgcaatgtccgaggaagaacgactcaaa
tatcttcgtttaaatccaaaggttatcactaacaaacaggacaaaggaaaatacaagttc
cttcaaaaatatttccatcgtggagctttcttcttggatgaagaagatgaagtactcaag
agaaactttgccgaagctaccaacgatgatcaatttgataaaactattctgccgaaagtt
atgcaggtcaaaaactttggaaaagcatctcgaaccaagtatacgcacttgacagaagaa
gatacaacagatcatcaaggtgtttgggcatcaacgaatcagttgaattctcaattctcc
acgaaaagagccggaggatccagaccagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGTGTTGATTTGTCATCTGTTC 101 419 868 Antisense
TTTGTCATCTGTTCTAACCGACGAT 201 667 879 Antisense
GAAGCCGCACGCGAGAAGGCAGAAC 614 343 982 Antisense
ACAAGATTCACGCAATGTCCGAGGA 10 113 1010 Antisense
GTTCCTTCAAAAATATTTCCATCGT 545 441 1104 Antisense
TTCCATCGTGGAGCTTTCTTCTTGG 369 701 1120 Antisense
AACTTTGCCGAAGCTACCAACGATG 231 139 1171 Antisense
GCATCTCGAACCAAGTATACGCACT 35 309 1252 Antisense
GGTGTTTGGGCATCAACGAATCAGT 640 499 1306 Antisense
GAATTCTCAATTCTCCACGAAAAGA 594 323 1332 Antisense
AAGAGCCGGAGGATCCAGACCAGTT 540 151 1353 Antisense
Sequence Source Affymetrix Proprietary Database