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[194017_s_at] Carbamoyl-phosphate synthase (CPSase) (2 domains), Biotin-requiring enzymes

Description F26D10.10 /REP_DB=WormBase Gene ID /WP=CE19810 /TR=Q9XVU2 /GB=CAB02317.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Carbamoyl-phosphate synthase (CPSase) (2 domains), Biotin-requiring enzymes
Cluster 6grp_total > 88      4grp_total > 08      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 12
(-2.7)
A 		9.1
(-3)
A 		23.9
(-1.5)
A 		67.4
(3)
A 		58
B 5.9
(-3)
A 		11.3
(-2.7)
A 		40.9
(-0.8)
A 		103.1
(3)
A 		97
C 8.9
(-3)
A 		8.8
(-3)
A 		19.9
(-2.3)
A 		108.7
(3)
A 		100
avr 8.9
(-3) 		9.7
(-2.9) 		28.2
(-1.6) 		93.1
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2496
1 2 3 4 5 6 range
A 13.9
(-3)
A 		22.3
(-3)
A 		10
(-3)
A 		152.6
(-2.6)
P 		1107.5
(-0.3)
P 		2431
(3)
P 		2421
B 15.8
(-3)
A 		11.3
(-3)
A 		16.3
(-3)
A 		119
(-2.7)
P 		1125.5
(-0.2)
P 		2410.9
(3)
P 		2400
C 4.8
(-3)
A 		27.9
(-2.9)
A 		34.8
(-2.9)
A 		126.4
(-2.7)
P 		1208.2
(-0.1)
P 		2501.1
(3)
P 		2496
avr 11.5
(-3) 		20.5
(-3) 		20.4
(-3) 		132.7
(-2.7) 		1147.1
(-0.2) 		2447.7
(3) 		2436
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 4.4
(-1.2)
A 		2
(-3)
A 		6.3
(0.3)
A 		9.9
(3)
A 		8
B 1.9
(-3)
A 		2.8
(-2.7)
A 		9.5
(-0.7)
A 		22
(3)
A 		20
C 2.9
(-2.8)
A 		2.5
(-3)
A 		6.9
(-1.3)
A 		18.3
(3)
A 		16
avr 3.1
(-2.7) 		2.4
(-3) 		7.6
(-0.8) 		16.7
(3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 897
1 2 3 4 5 6 range
A 2.6
(-3)
A 		7.9
(-2.9)
A 		2.2
(-3)
A 		38.7
(-2.6)
P 		334.2
(0.4)
P 		591.9
(3)
P 		590
B 5.5
(-3)
A 		3.8
(-3)
A 		4
(-3)
A 		31.5
(-2.8)
P 		457.3
(0)
P 		899
(3)
P 		895
C 1.8
(-3)
A 		8.5
(-3)
A 		8.8
(-2.9)
A 		25.4
(-2.8)
P 		391
(-0.2)
P 		837.3
(3)
P 		836
avr 3.3
(-3) 		6.7
(-3) 		5
(-3) 		31.9
(-2.8) 		394.2
(0) 		776.1
(3) 		773
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194017_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9201
Sequence Type Exemplar sequence
Representative Public ID F26D10.10 NCBI
Target Description F26D10.10 /REP_DB=WormBase Gene ID /WP=CE19810 /TR=Q9XVU2 /GB=CAB02317.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Carbamoyl-phosphate synthase (CPSase) (2 domains), Biotin-requiring enzymes
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070664(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070664 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-5) (gln-5) mRNA, complete cds. 11/11 None
SNAP00000006839 ENSEMBL cdna:SNAP chromosome:CEL140:IV:17272337:17273642:-1 11/11 None
GENEFINDER00000006852 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:17272337:17273588:-1 11/11 None
F26D10.10 ENSEMBL cdna:known chromosome:CEL140:IV:17272212:17273923:-1 gene:F26D10.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17272342-17273594(-) 100.0 100.0
Public Domain and Genome References
Gene Title Carbamoyl-phosphate synthase (CPSase) (2 domains), Biotin-requiring enzymes
Gene Symbol gln-5
Ensembl F26D10.10
Entrez Gene 178505 Entrez gene
SwissProt Q9XVU2 EMBL-EBI
WormBase CE19810 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250100_AT glutamine synthetase, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02317 Hypothetical protein F26D10.10 [Caenorhabditis elegans] ref|NP_503065.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-5) [Caenorhabditis elegans] 0.0
blast CAB05127 Hypothetical protein C28D4.3 [Caenorhabditis elegans] ref|NP_501733.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-6) [Caenorhabditis elegans] 0.0
blast CAE72665 Hypothetical protein CBG19879 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 2.2E-30
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 2.7E-95
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 2.2E-30
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 2.7E-95
Sequence
Target Sequence 
>C. ELEGANS:194017_S_AT
ggagtgtgcgtttcattggatcccaagccaaaggttaccatgggagactggaacggtgcc
ggttgtcataccaacttctcgacaattgaaatgcgaagaccaaacggtctgaacgccatt
ttcgaagctatgaaaggcctcgaaagaactcattcggaagccatgtcggtttacgatcca
aatggaggacgggacaatcttcgacgcctcaccggtcgccacgagactagtcaagctgac
aagttctcctggggaatcgccaatcgtgcgtgctcaattcggattccacgtcaagtcgct
gacgagacgaagggctatctcgaagaccgtcgtccatcatccaactgcgatccttacctg
gtgacagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTGTGCGTTTCATTGGATCCCA 278 517 721 Antisense
GGATCCCAAGCCAAAGGTTACCATG 412 513 738 Antisense
TGGAACGGTGCCGGTTGTCATACCA 99 547 769 Antisense
CAAACGGTCTGAACGCCATTTTCGA 13 191 821 Antisense
GAACTCATTCGGAAGCCATGTCGGT 680 349 866 Antisense
TGGAGGACGGGACAATCTTCGACGC 614 543 903 Antisense
TCAAGCTGACAAGTTCTCCTGGGGA 704 629 951 Antisense
CCAATCGTGCGTGCTCAATTCGGAT 274 277 980 Antisense
AATTCGGATTCCACGTCAAGTCGCT 613 175 996 Antisense
GAAGGGCTATCTCGAAGACCGTCGT 572 335 1029 Antisense
TGCGATCCTTACCTGGTGACAGCAA 702 577 1066 Antisense
Sequence Source Affymetrix Proprietary Database