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[194014_s_at]

Description F28H6.1 /REP_DB=WormBase Gene ID /WP=CE18646 /GEN=akt-2 /TR=Q9XTG7 /GB=CAB07403.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain, Protein kinase C terminal domain
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 57      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 83      6grp_total > 58      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 28      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 667.6
(1.6)
P 		557
(-1.3)
P 		494.3
(-3)
P 		721.5
(3)
P 		227
B 730.7
(3)
P 		530.6
(-0.8)
P 		414.2
(-3)
P 		634
(1.2)
P 		317
C 625
(0.9)
P 		536.7
(-2.3)
P 		516.9
(-3)
P 		681.9
(3)
P 		165
avr 674.4
(2.9) 		541.4
(-1.1) 		475.1
(-3) 		679.1
(3) 		204

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 447
1 2 3 4 5 6 range
A 563.3
(0.3)
P 		432.6
(-1.5)
P 		319.9
(-3)
P 		382.2
(-2.2)
P 		392
(-2)
P 		767.3
(3)
P 		447
B 524.3
(0.9)
P 		368.9
(-2.5)
P 		358.5
(-2.7)
P 		368.5
(-2.5)
P 		346.9
(-3)
P 		623
(3)
P 		276
C 626
(2.5)
P 		390.8
(-2.4)
P 		363.4
(-3)
P 		364.7
(-3)
P 		363
(-3)
P 		652.3
(3)
P 		289
avr 571.2
(1) 		397.4
(-2.1) 		347.3
(-3) 		371.8
(-2.6) 		367.3
(-2.6) 		680.9
(3) 		334
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 243.1
(3)
P 		120.9
(-2.3)
P 		129.2
(-2)
P 		105.6
(-3)
P 		138
B 233
(3)
P 		131
(-1.5)
P 		96
(-3)
P 		135.1
(-1.3)
P 		137
C 206.9
(3)
P 		154.3
(-0.4)
P 		180.1
(1.3)
P 		115
(-3)
P 		92
avr 227.7
(3) 		135.4
(-2.1) 		135.1
(-2.1) 		118.6
(-3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 103.8
(-1.3)
P 		153.8
(1.3)
P 		69.7
(-3)
P 		96.9
(-1.6)
P 		118.3
(-0.5)
P 		186.8
(3)
P 		117
B 182.4
(0.9)
P 		122.8
(-1.5)
P 		87
(-3)
P 		97.6
(-2.6)
P 		140.9
(-0.8)
P 		232.3
(3)
P 		145
C 232
(3)
P 		119.3
(-1.3)
P 		92.5
(-2.3)
P 		73.3
(-3)
P 		117.5
(-1.3)
P 		218.4
(2.5)
P 		159
avr 172.7
(1.2) 		132
(-0.7) 		83.1
(-3) 		89.3
(-2.7) 		125.6
(-1) 		212.5
(3) 		129
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194014_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17664
Sequence Type Exemplar sequence
Representative Public ID F28H6.1 NCBI
Target Description F28H6.1 /REP_DB=WormBase Gene ID /WP=CE18646 /GEN=akt-2 /TR=Q9XTG7 /GB=CAB07403.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain, Protein kinase C terminal domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029441(11), NM_077956(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029441 NCBI Caenorhabditis elegans AKT kinase family member (akt-2) (akt-2) mRNA, complete cds. 11/11 None
NM_077956 NCBI Caenorhabditis elegans AKT kinase family member (akt-2) (akt-2) mRNA, complete cds. 11/11 A 
SNAP00000040380 ENSEMBL cdna:SNAP chromosome:CEL140:X:14150228:14151480:1 11/11 None
GENEFINDER00000040389 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14150228:14151480:1 11/11 None
F28H6.1b.1 ENSEMBL cdna:known chromosome:CEL140:X:14147618:14151990:1 gene:F28H6.1 11/11 None
F28H6.1b.2 ENSEMBL cdna:known chromosome:CEL140:X:14147620:14151990:1 gene:F28H6.1 11/11 None
F28H6.1a ENSEMBL cdna:known chromosome:CEL140:X:14147627:14151862:1 gene:F28H6.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14147626-14151480(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28H6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC70087 Hypothetical protein F28H6.1b [Caenorhabditis elegans] emb|CAD21654.1| Hypothetical protein F28H6.1b [Caenorhabditis elegans] ref|NP_001024612.1| AKT kinase family member (akt-2) [Caenorhabditis elegans] gb|AAC62468.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAA20936 Hypothetical protein F28H6.1a [Caenorhabditis elegans] emb|CAB07403.1| Hypothetical protein F28H6.1a [Caenorhabditis elegans] ref|NP_510357.3| AKT kinase family member (akt-2) [Caenorhabditis elegans] 0.0
blast CAA98240 Hypothetical protein C12D8.10a [Caenorhabditis elegans] ref|NP_001023645.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62466.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-67
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 6.0E-99
hanks 1.3.2 AGC group; AGC III RAC(Akt); Akt2/Racb 7.0E-68
hanks 1.3.1 AGC group; AGC III RAC(Akt); Akt1/Raca 3.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.3E-87
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.7E-21
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 3.5E-12
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.0E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.5E-63
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.0E-19
Sequence
Target Sequence 
>C. ELEGANS:194014_S_AT
gcggaacaccagaataccttgcaccagaggttatcgaagacatcgactatgaccgtagtg
ttgactggtggggagtcggagtggtcatgtacgaaatgatgtgtggacggcttccatttt
cagcaaaggagaacggcaaactcttcgaactaattacgacatgtgatttgaagttcccga
ataggttaagcccagaagccgtaacattactatctggactactcgaaagggtcccagcta
agcggcttggtgccggtcctgatgatgcacgtgaagtttcgagagctgaatttttcaaag
atgtggattgggaagcaacattaagaaaagaagtcgaacctccatttaagccaaatgtga
tgtctgaaacggacacgagtttctttgacagagagttcacatcaatgccggtccaactga
cgcctccacgtagaggggaagaacttccgacagttgatgaggaagaagaattacaagcca
acttcatacaatttgcgtcatactacgtgtcggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGGAACACCAGAATACCTTGCACC 209 293 1028 Antisense
GTGGGGAGTCGGAGTGGTCATGTAC 121 489 1095 Antisense
TGTGGACGGCTTCCATTTTCAGCAA 75 555 1129 Antisense
GGAGAACGGCAAACTCTTCGAACTA 155 519 1155 Antisense
AAGCCCAGAAGCCGTAACATTACTA 147 149 1215 Antisense
AACATTACTATCTGGACTACTCGAA 390 137 1230 Antisense
CAGCTAAGCGGCTTGGTGCCGGTCC 416 191 1262 Antisense
TGCCGGTCCTGATGATGCACGTGAA 277 585 1278 Antisense
AGAAGTCGAACCTCCATTTAAGCCA 289 69 1356 Antisense
AACTGACGCCTCCACGTAGAGGGGA 132 137 1442 Antisense
ATTTGCGTCATACTACGTGTCGGGA 700 15 1518 Antisense
Sequence Source Affymetrix Proprietary Database