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[194001_s_at]

Description W09H1.6A /REP_DB=WormBase Gene ID /WP=CE16576 /GEN=lec-1 /TR=SW:P36573 /GB=CAB04959.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Vertebrate galactoside-binding lectins (2 domains)
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_high_8_8 > 52      6grp_highest_nonorm > 51      6grp_total > 32      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 739
1 2 3 4 range
A 625.2
(2.2)
P 		661
(2.5)
P 		714.4
(3)
P 		84.7
(-3)
A 		630
B 658.1
(2.4)
P 		694.7
(2.9)
P 		707.1
(3)
P 		197.5
(-3)
P 		510
C 538.7
(0.5)
P 		823.5
(3)
P 		727.8
(2.1)
P 		150.7
(-3)
P 		673
avr 607.3
(1.8) 		726.4
(3) 		716.4
(2.9) 		144.3
(-3) 		582

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 552
1 2 3 4 5 6 range
A 603.8
(2.1)
P 		601
(2)
P 		658.7
(3)
P 		496
(0.3)
P 		436.8
(-0.7)
P 		297.5
(-3)
P 		361
B 705.9
(2.1)
P 		540.7
(0.2)
P 		778.7
(3)
P 		468.5
(-0.7)
P 		433.2
(-1.1)
P 		271.8
(-3)
P 		507
C 548
(0.8)
P 		527.4
(0.6)
P 		731.5
(3)
P 		507.2
(0.3)
P 		633.6
(1.8)
P 		226.6
(-3)
P 		505
avr 619.2
(1.6) 		556.4
(0.8) 		723
(3) 		490.6
(-0) 		501.2
(0.1) 		265.3
(-3) 		458
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 227.6
(3)
P 		143.5
(0.7)
P 		186.8
(1.9)
P 		12.4
(-3)
A 		215
B 209.9
(3)
P 		171.6
(1.6)
P 		163.8
(1.4)
P 		42.1
(-3)
P 		168
C 178.3
(1)
P 		236.8
(2.6)
P 		253.7
(3)
P 		25.4
(-3)
P 		228
avr 205.3
(3) 		184
(2.3) 		201.4
(2.9) 		26.6
(-3) 		179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 111.3
(-1.3)
P 		213.8
(3)
P 		143.4
(0)
P 		125.8
(-0.7)
P 		131.8
(-0.5)
P 		72.4
(-3)
P 		141
B 245.6
(3)
P 		180
(0.3)
P 		189.1
(0.6)
P 		124.1
(-2.1)
P 		176
(0.1)
P 		101.4
(-3)
P 		144
C 203.1
(2.9)
P 		160.9
(1)
P 		186.2
(2.1)
P 		102
(-1.8)
P 		205
(3)
P 		75.9
(-3)
P 		129
avr 186.7
(3) 		184.9
(2.9) 		172.9
(2.2) 		117.3
(-1) 		170.9
(2.1) 		83.2
(-3) 		104
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 194001_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3927
Sequence Type Exemplar sequence
Representative Public ID W09H1.6A NCBI
Target Description W09H1.6A /REP_DB=WormBase Gene ID /WP=CE16576 /GEN=lec-1 /TR=SW:P36573 /GB=CAB04959.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Vertebrate galactoside-binding lectins (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064400(11), NM_001027234(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064400 NCBI Caenorhabditis elegans W09H1.6a (galectin) mRNA, complete cds. 11/11 A 
NM_001027234 NCBI Caenorhabditis elegans W09H1.6b (galectin) mRNA, complete cds. 11/11 None
SNAP00000010990 ENSEMBL cdna:SNAP chromosome:CEL140:II:13197181:13199374:1 11/11 None
GENEFINDER00000010995 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13195475:13199374:1 11/11 None
W09H1.6a ENSEMBL cdna:known chromosome:CEL140:II:13195445:13199607:1 gene:W09H1.6 11/11 A 
W09H1.6b ENSEMBL cdna:known chromosome:CEL140:II:13196927:13199374:1 gene:W09H1.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13197926-13199371(+) 96.79 96.79
Public Domain and Genome References
Ensembl W09H1.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04960 Hypothetical protein W09H1.6b [Caenorhabditis elegans] ref|NP_001022405.1| W09H1.6b [Caenorhabditis elegans] 1.0E-167
blast CAB04959 Hypothetical protein W09H1.6a [Caenorhabditis elegans] ref|NP_496801.2| W09H1.6a [Caenorhabditis elegans] sp|P36573|LEC1_CAEEL 32 kDa beta-galactoside-binding lectin (32 kDa GBP) gb|AAB87718.1| beta-galactoside binding protein [Caenorhabditis elegans] dbj|BAA22942.1| 32-kDa galectin [Caenorhabditis elegans] 1.0E-162
blast CAE59442 Hypothetical protein CBG02815 [Caenorhabditis briggsae] 1.0E-159
blast CAB04960 Hypothetical protein W09H1.6b [Caenorhabditis elegans] ref|NP_001022405.1| W09H1.6b [Caenorhabditis elegans] 1.0E-157
blast CAB04959 Hypothetical protein W09H1.6a [Caenorhabditis elegans] ref|NP_496801.2| W09H1.6a [Caenorhabditis elegans] sp|P36573|LEC1_CAEEL 32 kDa beta-galactoside-binding lectin (32 kDa GBP) gb|AAB87718.1| beta-galactoside binding protein [Caenorhabditis elegans] dbj|BAA22942.1| 32-kDa galectin [Caenorhabditis elegans] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 7.3E-35
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.4E-67
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 7.3E-35
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 8.8E-23
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 4.9E-37
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.4E-67
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 7.3E-35
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.4E-67
Sequence
Target Sequence 
>C. ELEGANS:194001_S_AT
gaagagcgcaagtcgaacccaatcaagaagggagactcgttcgacattcgcatccgtgct
cacgacgatcgcttccagatcatcgtcgaccacaaggagttcaaggactacgagcaccgt
ctgccactctcctcaatctctcacctctcgattgacggagatctctacttgaaccacgtt
cactggggaggaaagtactacccagtaccatacgagagcggactcgccaacggacttcca
gtcggaaagagcctcctcgtctttggaaccgttgagaagaaggccaagcgcttccacgtc
aaccttctccgcaagaatggagacatctcgttccacttcaacccacgtttcgatgagaag
cacgtcatccgcaactctcttgccgccaacgaatggggaaatgaggagcgcgagggaaag
aacccattcgagaagggagtcggcttcgacttggtcatccaaaacgaggagtacgcgttc
caggtgttcgtcaacggagaacgctacatctcgttcgctcaccgcgccgatccacacgac
attgccgggcttcagatctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGCGCAAGTCGAACCCAATCA 409 339 256 Antisense
AAGGGAGACTCGTTCGACATTCGCA 663 159 283 Antisense
CTTCCAGATCATCGTCGACCACAAG 382 219 327 Antisense
TTCAAGGACTACGAGCACCGTCTGC 607 683 355 Antisense
TCTCTCACCTCTCGATTGACGGAGA 662 611 392 Antisense
AGATCTCTACTTGAACCACGTTCAC 531 65 414 Antisense
TACGAGAGCGGACTCGCCAACGGAC 366 645 466 Antisense
AGAAGAAGGCCAAGCGCTTCCACGT 677 71 530 Antisense
GAATGGAGACATCTCGTTCCACTTC 444 333 570 Antisense
GGAGAACGCTACATCTCGTTCGCTC 32 519 751 Antisense
ACGACATTGCCGGGCTTCAGATCTC 414 91 791 Antisense
Sequence Source Affymetrix Proprietary Database