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[193981_s_at]

Description ZK256.1B /REP_DB=WormBase Gene ID /WP=CE20426 /TR=Q9XTG4 /GB=CAB05001.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=CALCIUM-TRANSPORTING ATPASE 1 (EC 3.6.1.38) (GOLGI CA2+-ATPASE)
Cluster 6grp_high1_10_10 > 27      4grp_high_8_8 > 25      6grp_total > 18      4grp_total > 46      6grp_cgc > 06      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 295.4
(3)
P 		226.8
(-0)
P 		158.4
(-3)
P 		163.2
(-2.8)
A 		137
B 320.4
(3)
P 		220.8
(-3)
P 		227.9
(-2.6)
P 		250.2
(-1.2)
P 		100
C 295.5
(3)
P 		193.2
(-2)
P 		172.9
(-3)
P 		181.6
(-2.6)
P 		123
avr 303.8
(3) 		213.6
(-1.6) 		186.4
(-3) 		198.3
(-2.4) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 318
1 2 3 4 5 6 range
A 211.8
(1)
P 		236
(2.2)
P 		132
(-3)
P 		188.4
(-0.2)
P 		196.8
(0.2)
P 		252.5
(3)
P 		121
B 183.2
(0.4)
P 		166
(-1.2)
A 		147.1
(-3)
P 		149.7
(-2.8)
P 		210.8
(3)
P 		199.9
(2)
P 		64
C 450
(3)
P 		168.9
(-2.5)
A 		288.5
(-0.2)
P 		209.3
(-1.7)
A 		142.6
(-3)
P 		208.4
(-1.7)
P 		307
avr 281.7
(3) 		190.3
(-2.5) 		189.2
(-2.6) 		182.5
(-3) 		183.4
(-2.9) 		220.3
(-0.7) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 107.6
(3)
P 		49.2
(-1.2)
P 		41.4
(-1.7)
P 		23.9
(-3)
A 		84
B 102.2
(3)
P 		54.5
(-2.8)
P 		52.8
(-3)
P 		53.3
(-2.9)
P 		49
C 97.8
(3)
P 		55.5
(-0.8)
P 		60.3
(-0.3)
P 		30.6
(-3)
P 		67
avr 102.5
(3) 		53.1
(-1.5) 		51.5
(-1.6) 		35.9
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 39
(-1.9)
P 		83.9
(3)
P 		28.7
(-3)
P 		47.8
(-0.9)
P 		59.4
(0.3)
P 		61.5
(0.6)
P 		55
B 63.7
(0.4)
P 		55.2
(-0.7)
A 		35.7
(-3)
P 		39.7
(-2.5)
P 		85.7
(3)
P 		74.5
(1.7)
P 		50
C 166.8
(3)
P 		51.5
(-2.5)
A 		73.4
(-1.5)
P 		42.1
(-3)
A 		46.1
(-2.8)
P 		69.8
(-1.7)
P 		125
avr 89.8
(3) 		63.5
(-0.4) 		45.9
(-2.7) 		43.2
(-3) 		63.7
(-0.4) 		68.6
(0.3) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193981_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.805
Sequence Type Exemplar sequence
Representative Public ID ZK256.1B NCBI
Target Description ZK256.1B /REP_DB=WormBase Gene ID /WP=CE20426 /TR=Q9XTG4 /GB=CAB05001.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=CALCIUM-TRANSPORTING ATPASE 1 (EC 3.6.1.38) (GOLGI CA2+-ATPASE)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026689(11), NM_001026691(11), NM_001026690(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026689 NCBI Caenorhabditis elegans PMR-type Golgi ATPase family member (pmr-1) (pmr-1) mRNA, complete cds. 11/11 None
NM_001026691 NCBI Caenorhabditis elegans PMR-type Golgi ATPase family member (pmr-1) (pmr-1) mRNA, complete cds. 11/11 None
NM_001026690 NCBI Caenorhabditis elegans PMR-type Golgi ATPase family member (pmr-1) (pmr-1) mRNA, complete cds. 11/11 None
ZK256.1a ENSEMBL cdna:known chromosome:CEL140:I:13012557:13031740:1 gene:ZK256.1 11/11 A 
ZK256.1c ENSEMBL cdna:known chromosome:CEL140:I:13019550:13031740:1 gene:ZK256.1 11/11 A 
ZK256.1b ENSEMBL cdna:known chromosome:CEL140:I:13020340:13029125:1 gene:ZK256.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000015700 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13020270-13028982(+) 98.77 100.0
Public Domain and Genome References
Ensembl ZK256.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAF32230 Hypothetical protein ZK256.1c [Caenorhabditis elegans] emb|CAF32231.1| Hypothetical protein ZK256.1c [Caenorhabditis elegans] ref|NP_001021862.1| PMR-type Golgi ATPase family member (pmr-1) [Caenorhabditis elegans] 0.0
blast CAB05000 Hypothetical protein ZK256.1a [Caenorhabditis elegans] emb|CAB04015.2| Hypothetical protein ZK256.1a [Caenorhabditis elegans] emb|CAC19896.1| PMR1 protein [Caenorhabditis elegans] emb|CAC19895.1| PMR1 protein [Caenorhabditis elegans] ref|NP_001021860.1| PMR-type Golgi ATPase family member (pmr-1) [Caenorhabditis elegans] 0.0
blast CAE63458 Hypothetical protein CBG07921 [Caenorhabditis briggsae] 0.0
blast CAB05001 Hypothetical protein ZK256.1b [Caenorhabditis elegans] emb|CAB04016.1| Hypothetical protein ZK256.1b [Caenorhabditis elegans] ref|NP_001021861.1| PMR-type Golgi ATPase family member (pmr-1) [Caenorhabditis elegans] pir||T20278 hypothetical protein ZK256.1b - Caenorhabditis elegans 0.0
Protein Families
Method ID Description E-Value
ec A1A3_HUMAN (P13637) Sodium/potassium-transporting ATPase alpha-3 chain (EC 3.6.3.9) (Sodium pump 3) (Na+/K+ ATPase 3) (Alpha(III)) 0.0
ec A1A1_HUMAN (P05023) Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+ ATPase 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 7.7E-100
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-25
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.1E-26
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 7.7E-100
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-25
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.1E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021860.1 8 65-82,87-104,255-272,287-309,757-779,794-811,832-854,858-877
NP_001021862.1 7 163-180,332-349,364-386,834-856,871-888,909-931,935-954
NP_001021861.1 4 65-82,87-104,255-272,287-309
ZK256.1a 8 65-82,87-104,255-272,287-309,757-779,794-811,832-854,858-877
ZK256.1c 7 163-180,332-349,364-386,834-856,871-888,909-931,935-954
ZK256.1b 4 65-82,87-104,255-272,287-309
Sequence
Target Sequence 
>C. ELEGANS:193981_S_AT
caaaaagggctccgtgtgctcggtttggcacgtggagagagtatgcaatctttgatgttt
ctgggaatgattggaatgatggacccaccacgtcctggtgctgcagatgcgattagtatt
gttaaagctagtggtgttgatgttaagcttatcactggagatgcaatggagacggctcag
agcattggtcaatccctcggtatcctctcatcctccgattcctgcctctccggccaacaa
gtcgatcaaatgtcggatcacgatctggagctagtcatccgtcaagttaccgtattctac
cgagcatcgccacgtcataagctgaaaatcgtgaaggcactgcaggcactcggtgaggtc
gtcgcaatgaccggagacggtgtaaatgatgctgtggctcttaaaaaagcagatattgga
gtggcaatgggtgtatgtggaacagatgtatgtaaagaagcagctgatatgatcctttgt
gatgatgatttttcaacgatgaccgcggcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAAAGGGCTCCGTGTGCTCGGTT 644 189 1522 Antisense
ATTGGAATGATGGACCCACCACGTC 502 3 1591 Antisense
CGTCCTGGTGCTGCAGATGCGATTA 483 245 1612 Antisense
CTCAGAGCATTGGTCAATCCCTCGG 186 227 1697 Antisense
TCTCCGGCCAACAAGTCGATCAAAT 267 611 1748 Antisense
CGGATCACGATCTGGAGCTAGTCAT 464 243 1775 Antisense
AGCTAGTCATCCGTCAAGTTACCGT 577 75 1790 Antisense
AAGTTACCGTATTCTACCGAGCATC 257 157 1805 Antisense
GCATCGCCACGTCATAAGCTGAAAA 568 309 1825 Antisense
CACTCGGTGAGGTCGTCGCAATGAC 584 197 1868 Antisense
ATTTTTCAACGATGACCGCGGCGAT 631 13 2009 Antisense
Sequence Source Affymetrix Proprietary Database