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[193978_at] serine/threonine kinase (KIN1/SNF1/Nim1 subfamily)

Description F15A2.6 /REP_DB=WormBase Gene ID /WP=CE28218 /GEN=sad-1 /TR=Q19469 /GB=CAA94127.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (KIN1-SNF1-Nim1 subfamily)
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 05      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 310
1 2 3 4 range
A 528.4
(3)
P 		325.9
(-0.9)
P 		218.3
(-3)
P 		228.9
(-2.8)
P 		310
B 504.1
(3)
P 		317.9
(-0.9)
P 		246.9
(-2.4)
P 		218.3
(-3)
P 		286
C 445.1
(3)
P 		324.4
(-0.5)
P 		243.9
(-2.8)
P 		236.3
(-3)
P 		209
avr 492.5
(3) 		322.7
(-0.8) 		236.4
(-2.8) 		227.8
(-3) 		265

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 308
1 2 3 4 5 6 range
A 387.4
(3)
P 		335.7
(1.8)
P 		120.9
(-3)
P 		185.1
(-1.6)
P 		183.1
(-1.6)
P 		209.8
(-1)
P 		267
B 294.3
(1.4)
P 		333
(3)
P 		209.8
(-2)
P 		184.2
(-3)
P 		234.4
(-1)
P 		190.4
(-2.8)
P 		149
C 428.5
(3)
P 		324.6
(0.6)
P 		230.6
(-1.5)
P 		307.6
(0.3)
P 		178.6
(-2.7)
P 		164.5
(-3)
P 		264
avr 370.1
(3) 		331.1
(1.7) 		187.1
(-3) 		225.6
(-1.7) 		198.7
(-2.6) 		188.2
(-3) 		183
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 159
1 2 3 4 range
A 192.4
(3)
P 		70.8
(-1.6)
P 		57.1
(-2.1)
P 		33.5
(-3)
P 		159
B 160.7
(3)
P 		78.5
(-1.3)
P 		57.2
(-2.4)
P 		46.5
(-3)
P 		114
C 147.3
(3)
P 		93.3
(-0)
P 		85
(-0.5)
P 		39.8
(-3)
P 		108
avr 166.8
(3) 		80.9
(-1.1) 		66.4
(-1.7) 		39.9
(-3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 71.4
(-0.1)
P 		119.4
(3)
P 		26.3
(-3)
P 		46.9
(-1.7)
P 		55.3
(-1.1)
P 		51.1
(-1.4)
P 		93
B 102.4
(2.2)
P 		110.9
(3)
P 		50.9
(-2.8)
P 		48.8
(-3)
P 		95.2
(1.5)
P 		71
(-0.9)
P 		62
C 158.8
(3)
P 		99
(-0.5)
P 		58.7
(-2.8)
P 		61.9
(-2.6)
P 		57.8
(-2.8)
P 		55.1
(-3)
P 		104
avr 110.9
(3) 		109.8
(2.9) 		45.3
(-3) 		52.5
(-2.3) 		69.4
(-0.8) 		59.1
(-1.7) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193978_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17774
Sequence Type Exemplar sequence
Representative Public ID F15A2.6 NCBI
Target Description F15A2.6 /REP_DB=WormBase Gene ID /WP=CE28218 /GEN=sad-1 /TR=Q19469 /GB=CAA94127.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (KIN1-SNF1-Nim1 subfamily)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077852(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077852 NCBI Caenorhabditis elegans Synapses of Amphids Defective family member (sad-1) (sad-1) mRNA, complete cds. 11/11 A 
SNAP00000028419 ENSEMBL cdna:SNAP chromosome:CEL140:X:13488007:13493557:-1 11/11 None
GENEFINDER00000028422 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13488007:13494814:-1 11/11 None
F15A2.6 ENSEMBL cdna:known chromosome:CEL140:X:13487483:13500569:-1 gene:F15A2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13488006-13494751(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase (KIN1/SNF1/Nim1 subfamily)
Gene Symbol sad-1
Ensembl F15A2.6
Entrez Gene 181471 Entrez gene
SwissProt Q19469 EMBL-EBI
WormBase CE28218 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.4766.1.S1_S_AT similar to BR serine/threonine-protein kinase 1 (SAD1 kinase) (SAD1A) cfa
DROSOPHILA_2:1634134_AT dm
DROSOPHILA_2:1623313_AT dm
DROSGENOME1:148833_AT dm
U133_X3P:HS2.182081.1.S1_3P_X_AT BR serine/threonine kinase 1 hs
U133_X3P:HS2.182081.1.S1_3P_S_AT BR serine/threonine kinase 1 hs
U133_X3P:HS2.182081.1.S1_3P_A_AT BR serine/threonine kinase 1 hs
U133_X3P:1552504_3P_A_AT BR serine/threonine kinase 1 hs
HG-U133_PLUS_2:1552504_A_AT BR serine/threonine kinase 1 hs
HG-U95E:67204_AT BR serine/threonine kinase 1 hs
MOE430B:1439892_AT BR serine/threonine kinase 1 mm
MOUSE430_2:1439892_AT BR serine/threonine kinase 1 mm
MG-U74BV2:105888_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
MU19KSUBB:TC30057_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
MU19KSUBB:TC30057_G_AT BR serine/threonine kinase 1, mRNA (cDNA clone MGC:99905 IMAGE:6402113) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
30424 axon inferred from direct assay QuickGO AmiGO
30425 dendrite inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from sequence similarity QuickGO AmiGO
5524 ATP binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94127 Hypothetical protein F15A2.6 [Caenorhabditis elegans] ref|NP_510253.1| Synapses of Amphids Defective family member (sad-1) [Caenorhabditis elegans] gb|AAG50270.1| serine/threonine kinase SAD-1 [Caenorhabditis elegans] 0.0
blast CAE63138 Hypothetical protein CBG07440 [Caenorhabditis briggsae] 0.0
blast AAF49569 CG6114-PA [Drosophila melanogaster] ref|NP_648814.3| CG6114-PA [Drosophila melanogaster] 1.0E-162
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-69
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-69
hanks 2.2.4 CaMK Group; CaMK II KIN1/SNF1/Nim1; p78 5.0E-85
hanks 2.2.4 CaMK Group; CaMK II KIN1/SNF1/Nim1; p78 2.0E-85
hanks 2.2.3 CaMK Group; CaMK II KIN1/SNF1/Nim1; kin1+ 2.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.6E-95
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-76
Pfam IPR000719 EMBL-EBI Protein kinase 8.6E-95
Sequence
Target Sequence 
>C. ELEGANS:193978_AT
tgttgatattatcccatctcctcaacaagtagtgatagctggagaaactccgacatatgt
agtacagtttgttctgctggcgggaccagttcggaggtttaaacgacttgtggaacactt
gtcagcaattctgcaaaattctactcaacaaagagctgatcgacaacaacaagcagccct
aatggttcgaccaaggaggttaagtgactccagcgttggtagtgcttgttcggacagtga
aagcaatgcgagcagtattaatatgatagcgcggcattcggataaaactgaaacaacttc
tgccacgtcatctgacccttacggtccatccccgtcaatgcgatctgttggaagtggaac
agctaactcatacaaatcgccaacccctcatcgccgtaacacgactgctgtcactgcttc
atcgtcatccgcatcaaaccgatatggtccaagcagtagctcgtctggtagttacagtaa
caatgctgactattcctatcatcccgagtattcacagagaagcaatggctcaagtgctcc
gaagaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTGATATTATCCCATCTCCTCAA 564 563 2064 Antisense
AGTACAGTTTGTTCTGCTGGCGGGA 93 63 2124 Antisense
GTGGAACACTTGTCAGCAATTCTGC 348 491 2173 Antisense
ACAAGCAGCCCTAATGGTTCGACCA 237 111 2232 Antisense
GTGACTCCAGCGTTGGTAGTGCTTG 360 483 2267 Antisense
ATCCCCGTCAATGCGATCTGTTGGA 566 37 2391 Antisense
GCTAACTCATACAAATCGCCAACCC 659 301 2425 Antisense
TCGTCATCCGCATCAAACCGATATG 261 605 2485 Antisense
GGTCCAAGCAGTAGCTCGTCTGGTA 272 499 2509 Antisense
TATTCCTATCATCCCGAGTATTCAC 483 665 2554 Antisense
ATGGCTCAAGTGCTCCGAAGAATCA 470 51 2588 Antisense
Sequence Source Affymetrix Proprietary Database