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[193970_s_at]

Description C47B2.7B /REP_DB=WormBase Gene ID /WP=CE24853 /TR=O62108 /GB=CAB16862.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Elongation factor Tu family (contains ATP-GTP binding P-loop)
Cluster 6grp_total > 86      4grp_total > 14      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 97.9
(0.3)
A 		12.7
(-3)
A 		16
(-2.9)
A 		169.2
(3)
A 		157
B 127.9
(2.6)
A 		14.8
(-3)
A 		43.5
(-1.6)
A 		135.8
(3)
P 		121
C 107
(2.4)
A 		22.4
(-2.6)
A 		14.7
(-3)
A 		117.4
(3)
A 		103
avr 110.9
(1.6) 		16.6
(-3) 		24.7
(-2.6) 		140.8
(3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 27.5
(-3)
A 		54.1
(-2.1)
A 		98.2
(-0.5)
A 		105.2
(-0.2)
A 		144.2
(1.2)
A 		195.7
(3)
P 		168
B 84.7
(-1.3)
A 		56.3
(-2.4)
A 		42.7
(-3)
A 		115.9
(0)
A 		129.6
(0.6)
A 		187.3
(3)
P 		145
C 56.6
(-2.2)
A 		29.5
(-3)
A 		113.9
(-0.4)
A 		82.4
(-1.4)
A 		150.1
(0.7)
A 		223.8
(3)
P 		194
avr 56.3
(-2.6) 		46.6
(-3) 		84.9
(-1.5) 		101.2
(-0.9) 		141.3
(0.6) 		202.3
(3) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 35.7
(3)
A 		2.8
(-3)
A 		4.2
(-2.7)
A 		24.8
(1)
A 		33
B 40.8
(3)
A 		3.7
(-3)
A 		10.1
(-2)
A 		28.9
(1.1)
P 		37
C 35.4
(3)
A 		6.4
(-2.7)
A 		5.1
(-3)
A 		19.8
(-0.1)
A 		30
avr 37.3
(3) 		4.3
(-3) 		6.5
(-2.6) 		24.5
(0.7) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 5.1
(-3)
A 		19.2
(-1)
A 		21.4
(-0.7)
A 		26.7
(0)
A 		43.5
(2.4)
A 		47.6
(3)
P 		43
B 29.5
(-1.1)
A 		18.7
(-2.2)
A 		10.4
(-3)
A 		30.7
(-0.9)
A 		52.6
(1.3)
A 		69.8
(3)
P 		59
C 21
(-1.9)
A 		9
(-3)
A 		29
(-1.2)
A 		16.6
(-2.3)
A 		48.6
(0.6)
A 		74.9
(3)
P 		66
avr 18.5
(-2.6) 		15.6
(-3) 		20.3
(-2.4) 		24.7
(-1.9) 		48.2
(1) 		64.1
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193970_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.385
Sequence Type Exemplar sequence
Representative Public ID C47B2.7B NCBI
Target Description C47B2.7B /REP_DB=WormBase Gene ID /WP=CE24853 /TR=O62108 /GB=CAB16862.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Elongation factor Tu family (contains ATP-GTP binding P-loop)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060875(11), NM_060874(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060875 NCBI Caenorhabditis elegans C47B2.7a (C47B2.7) mRNA, complete cds. 11/11 None
NM_060874 NCBI Caenorhabditis elegans C47B2.7b (C47B2.7) mRNA, complete cds. 11/11 None
GENEFINDER00000041238 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12988691:12991992:1 11/11 None
C47B2.7a ENSEMBL cdna:known chromosome:CEL140:I:12986959:12990560:1 gene:C47B2.7 11/11 None
C47B2.7b ENSEMBL cdna:known chromosome:CEL140:I:12986959:12990541:1 gene:C47B2.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041236 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12986889-12990472(+) 98.4 100.0
Public Domain and Genome References
Ensembl C47B2.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB16863 Hypothetical protein C47B2.7a [Caenorhabditis elegans] ref|NP_493276.1| C47B2.7a [Caenorhabditis elegans] 0.0
blast CAB16862 Hypothetical protein C47B2.7b [Caenorhabditis elegans] ref|NP_493275.1| C47B2.7b [Caenorhabditis elegans] 0.0
blast CAE63467 Hypothetical protein CBG07934 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 7.0E-10
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 7.0E-10
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 1.8E-15
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 7.0E-10
Pfam IPR002942 EMBL-EBI RNA-binding S4 1.1E-12
Sequence
Target Sequence 
>C. ELEGANS:193970_S_AT
tgctggcaaccgtggaacctatccagttttttagaaaatcaataaattccaaatcgaaaa
tccacgtggcagtcgcttttgagaccgttatggcggaatgtcaatttctcaaagaaaagg
aaaatggagaatttgaaatgcttccaagtctcctggcaccgtgccaagtgctattttcat
tcgaaaaatcgatatttctacccgaaaactacagtaacccgttcatggccgcccgtctcg
aacaacaaccgggcagtggatgccgtttcgctttttccggaattttcagccaaattttgc
ctgaaaatgggaaaaatttgccgccgagatttacgagaaaatgcagaaaaggacatgtgg
aaagagtggaaaaagatggattttcggcgatttgtactggaatgttcaaggccgaaacga
attttgacgtttttcgcggttttcagatctgcacctcatctggtccacgtggaaaaatcg
aaggcgctttcggaaaatcgggcaaatttcgtgtaaccttcgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGGCAACCGTGGAACCTATCCA 205 583 893 Antisense
TCCACGTGGCAGTCGCTTTTGAGAC 132 587 953 Antisense
ATTTGAAATGCTTCCAAGTCTCCTG 599 17 1023 Antisense
ACCGTGCCAAGTGCTATTTTCATTC 322 89 1050 Antisense
CCGTCTCGAACAACAACCGGGCAGT 92 265 1125 Antisense
AACCGGGCAGTGGATGCCGTTTCGC 652 141 1139 Antisense
AATTTGCCGCCGAGATTTACGAGAA 549 173 1207 Antisense
GACGTTTTTCGCGGTTTTCAGATCT 274 369 1318 Antisense
TTTCAGATCTGCACCTCATCTGGTC 286 673 1333 Antisense
GAAGGCGCTTTCGGAAAATCGGGCA 628 329 1372 Antisense
GCAAATTTCGTGTAACCTTCGCCGA 694 317 1394 Antisense
Sequence Source Affymetrix Proprietary Database