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[193961_at]

Description B0207.3 /REP_DB=WormBase Gene ID /WP=CE27564 /GEN=gpa-14 /TR=O01428 /GB=AAB52458.3 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein, alpha subunit
Cluster 6grp_total > 35      4grp_total > 14      6grp_cgc > 34      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 133.9
(-0.1)
P 		51.9
(-2.7)
A 		42.9
(-3)
A 		229
(3)
A 		186
B 197.5
(3)
M 		60.9
(-2.4)
A 		46.8
(-3)
A 		169.6
(1.9)
M 		151
C 172.4
(0.9)
P 		64.4
(-2.3)
A 		40.9
(-3)
A 		244.5
(3)
A 		204
avr 167.9
(1.4) 		59.1
(-2.5) 		43.5
(-3) 		214.4
(3) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 158.1
(2.8)
A 		106.7
(-0.3)
P 		78.3
(-2)
A 		61.1
(-3)
A 		161.3
(3)
A 		71.4
(-2.4)
A 		100
B 146.3
(2.6)
P 		152.8
(3)
A 		151.7
(2.9)
P 		104.2
(-0.2)
A 		62.1
(-3)
P 		101.3
(-0.4)
M 		91
C 110.6
(0.1)
A 		182.4
(3)
P 		145.3
(1.5)
P 		112.9
(0.1)
A 		36.3
(-3)
A 		124.4
(0.6)
P 		146
avr 138.3
(2.1) 		147.3
(3) 		125.1
(0.8) 		92.7
(-2.4) 		86.6
(-3) 		99
(-1.8) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 48.8
(3)
P 		11.3
(-3)
A 		11.2
(-3)
A 		33.5
(0.6)
A 		38
B 63
(3)
M 		15
(-2.5)
A 		10.8
(-3)
A 		36.1
(-0.1)
M 		52
C 57.1
(3)
P 		18.5
(-2.4)
A 		14.3
(-3)
A 		41.2
(0.8)
A 		43
avr 56.3
(3) 		14.9
(-2.6) 		12.1
(-3) 		36.9
(0.4) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 29.1
(-0.5)
A 		37.9
(1)
P 		17
(-2.7)
A 		15.5
(-3)
A 		48.7
(3)
A 		17.4
(-2.7)
A 		33
B 50.9
(3)
P 		50.9
(3)
A 		36.8
(-0.3)
P 		27.6
(-2.4)
A 		25.2
(-3)
P 		37.8
(-0.1)
M 		26
C 41
(1)
A 		55.6
(3)
P 		37
(0.5)
P 		22.7
(-1.5)
A 		11.7
(-3)
A 		41.7
(1.1)
P 		44
avr 40.3
(1.2) 		48.1
(3) 		30.3
(-1.1) 		21.9
(-3) 		28.5
(-1.5) 		32.3
(-0.6) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193961_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.866
Sequence Type Exemplar sequence
Representative Public ID B0207.3 NCBI
Target Description B0207.3 /REP_DB=WormBase Gene ID /WP=CE27564 /GEN=gpa-14 /TR=O01428 /GB=AAB52458.3 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein, alpha subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025795(9), NM_001025794(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025795 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-14) (gpa-14) mRNA, complete cds. 9/11 None
NM_001025794 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-14) (gpa-14) mRNA, complete cds. 9/11 None
GENEFINDER00000009183 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5950554:5953672:1 9/11 None
SNAP00000009171 ENSEMBL cdna:SNAP chromosome:CEL140:I:5950712:5953672:1 9/11 None
B0207.3b ENSEMBL cdna:known chromosome:CEL140:I:5950712:5953672:1 gene:B0207.3 9/11 None
B0207.3a ENSEMBL cdna:known chromosome:CEL140:I:5950712:5953672:1 gene:B0207.3 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5950711-5953672(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0207.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001020966 G Protein, Alpha subunit family member (gpa-14) [Caenorhabditis elegans] 0.0
blast AAP68962 G protein, alpha subunit protein 14, isoform b [Caenorhabditis elegans] 0.0
blast AAB52458 G protein, alpha subunit protein 14, isoform a [Caenorhabditis elegans] ref|NP_001020965.1| G Protein, Alpha subunit family member (gpa-14) [Caenorhabditis elegans] gb|AAG32089.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9BIG2|GPA14_CAEEL Guanine nucleotide-binding protein alpha-14 subunit 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 9.6E-58
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 4.4E-21
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 4.8E-59
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 4.4E-21
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.2E-57
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 4.4E-21
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.7E-5
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 3.9E-43
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 4.4E-21
Sequence
Target Sequence 
>C. ELEGANS:193961_AT
tcattactcaacatcctagtgaaactgaaaattttagattttttcctcttttatttcaga
atgattgacgtgggtggtcaaagatcggaacgaaagaaatggttgcatttgtttgatgat
gccaaggttgtactttttgtgattgatttgacaggttatgcgaaaagatcagaagaaagt
cgaatggaggtaaaatacttcgaaacttttcagtttaaaatttctagaacttggatattg
agaattctaagtcgatttccaaaattctttcgagacgttggatcaaatgcctatgatatg
aaagttgcactcaagattttcaatgaagttgcagcagcaagtgcattggcaaacgctgta
tttttattattttttaacaaagttgacttgttcaaagaaattctttcacaggtcaatctt
cagccttgcttttccaaatttgacggagagaatacttacgaagagacgagtaaatacatt
tgtgaaaagtttattcgagcagctagctcgaaaaaatcagtattcccccatttcacaaca
gcaacaaacacggaaaatgttaagttagtatttcgcgcctgcatggaaagtgtattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATTACTCAACATCCTAGTGAAAC 262 615 598 Antisense
TTTTTTCCTCTTTTATTTCAGAATG 646 675 636 Antisense
GATGCCAAGGTTGTACTTTTTGTGA 359 413 715 Antisense
AATTCTTTCGAGACGTTGGATCAAA 405 177 860 Antisense
GTGCATTGGCAAACGCTGTATTTTT 104 481 938 Antisense
GAAATTCTTTCACAGGTCAATCTTC 600 363 994 Antisense
CACAGGTCAATCTTCAGCCTTGCTT 528 195 1004 Antisense
CAGCCTTGCTTTTCCAAATTTGACG 424 203 1018 Antisense
CCCCATTTCACAACAGCAACAAACA 643 261 1123 Antisense
TTAAGTTAGTATTTCGCGCCTGCAT 83 683 1157 Antisense
TCGCGCCTGCATGGAAAGTGTATTT 16 601 1170 Antisense
Sequence Source Affymetrix Proprietary Database