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[193957_s_at]

Description T14G12.4 /REP_DB=WormBase Gene ID /WP=CE04965 /TR=Q22510 /GB=AAA82436.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=fork head domain transcription factor (SLP2, D. melanogaster)
Cluster 6grp_total > 08      4grp_total > 63      6grp_cgc > 0      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 130.2
(3)
P 		85.5
(-3)
A 		120.7
(1.7)
A 		106.8
(-0.1)
A 		45
B 102.8
(3)
A 		60.1
(-3)
A 		96.3
(2.1)
A 		70.4
(-1.6)
A 		43
C 32.4
(-3)
P 		79.8
(3)
P 		71.4
(1.9)
A 		65.5
(1.2)
A 		47
avr 88.5
(0.8) 		75.1
(-3) 		96.1
(3) 		80.9
(-1.3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 104.2
(2.1)
A 		78
(-0.5)
A 		113
(3)
M 		99.5
(1.6)
M 		76.7
(-0.7)
A 		53.7
(-3)
A 		59
B 160.5
(3)
P 		86.7
(0.1)
M 		8.4
(-3)
A 		63.5
(-0.8)
A 		59.8
(-1)
A 		71.5
(-0.5)
A 		152
C 55.5
(-1.9)
P 		68.4
(-0.4)
A 		83.1
(1.2)
A 		98.7
(3)
A 		45.7
(-3)
A 		72.3
(0)
A 		53
avr 106.7
(3) 		77.7
(-0.8) 		68.2
(-2) 		87.2
(0.5) 		60.7
(-3) 		65.8
(-2.3) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 47.4
(3)
P 		18.6
(-2.4)
A 		31.6
(0)
A 		15.6
(-3)
A 		32
B 32.8
(3)
A 		14.8
(-3)
A 		22.3
(-0.5)
A 		15
(-2.9)
A 		18
C 10.7
(-3)
P 		23
(2.2)
P 		24.9
(3)
A 		11
(-2.9)
A 		14
avr 30.3
(3) 		18.8
(-1.2) 		26.3
(1.5) 		13.9
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 19.2
(-0.5)
A 		27.8
(3)
A 		24.6
(1.7)
M 		25.2
(1.9)
M 		23.1
(1.1)
A 		13.1
(-3)
A 		15
B 55.8
(3)
P 		28.9
(0)
M 		2
(-3)
A 		16.8
(-1.3)
A 		24.3
(-0.5)
A 		26.7
(-0.2)
A 		54
C 20.6
(0.7)
P 		20.9
(0.9)
A 		21.1
(1)
A 		19.9
(0.3)
A 		14.8
(-3)
A 		24.2
(3)
A 		9
avr 31.9
(3) 		25.9
(0.8) 		15.9
(-3) 		20.6
(-1.2) 		20.7
(-1.2) 		21.3
(-1) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193957_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18618
Sequence Type Exemplar sequence
Representative Public ID T14G12.4 NCBI
Target Description T14G12.4 /REP_DB=WormBase Gene ID /WP=CE04965 /TR=Q22510 /GB=AAA82436.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=fork head domain transcription factor (SLP2, D. melanogaster)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076243(11), NM_076244(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076243 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-2) (fkh-2) mRNA, complete cds. 11/11 None
NM_076244 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-2) (fkh-2) mRNA, complete cds. 11/11 None
SNAP00000013394 ENSEMBL cdna:SNAP chromosome:CEL140:X:3740557:3742240:-1 11/11 None
GENEFINDER00000013405 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3740557:3742240:-1 11/11 None
T14G12.4a ENSEMBL cdna:known chromosome:CEL140:X:3740225:3742273:-1 gene:T14G12.4 11/11 A 
T14G12.4b.2 ENSEMBL cdna:known chromosome:CEL140:X:3740557:3742238:-1 gene:T14G12.4 11/11 None
T14G12.4b.1 ENSEMBL cdna:known chromosome:CEL140:X:3740557:3742233:-1 gene:T14G12.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3740557-3742241(-) 100.0 100.0
Public Domain and Genome References
Ensembl T14G12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508644 ForKHead transcription factor family member (fkh-2) [Caenorhabditis elegans] gb|AAA82436.1| Forkhead transcription factor family protein 2, isoform a [Caenorhabditis elegans] 1.0E-119
blast CAE68479 Hypothetical protein CBG14280 [Caenorhabditis briggsae] 6.0E-92
blast NP_508645 ForKHead transcription factor family member (fkh-2) [Caenorhabditis elegans] gb|AAL02521.1| Forkhead transcription factor family protein 2, isoform b [Caenorhabditis elegans] 3.0E-88
blast NP_508644 ForKHead transcription factor family member (fkh-2) [Caenorhabditis elegans] gb|AAA82436.1| Forkhead transcription factor family protein 2, isoform a [Caenorhabditis elegans] 3.0E-88
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.00000002044225E-38
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 3.000000056533E-31
Pfam IPR001766 EMBL-EBI Fork head transcription factor 2.1E-64
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.2E-53
Sequence
Target Sequence 
>C. ELEGANS:193957_S_AT
gcaaggattccaagtcaccatcatccagcaactcagaagagaaaactccaagctctccga
acgacaagccaccattctcctacaatgcattgatcatgatggccatcaaagacagtccag
aaaaacgtctcacattggctggaatttacgaatacatcgtcaccaattacccattctacc
gggacaacaagcagggatggcaaaactccatccgccacaacctctccctcaacaagtgct
ttgtcaaagttccaagaaactttgatgatccaggcaaagggaactattggatgctcgatg
caactgctgaggacgaggtattcattggtggagcaactggaaaactgcgccgtcgccctt
cctctctctcgcgtgctagaatggacgcctacaagcagtacggagcagccgctgcaaacc
tcttcccatacttcagtccaggaatgccagccctccctcgtcacccgtatctgactgctc
caaatggctttctccctcgtcaaatgatgccgattccagcgatgcctcaagttttcgcac
aacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAGGATTCCAAGTCACCATCATC 430 321 224 Antisense
GAAAAACGTCTCACATTGGCTGGAA 78 355 343 Antisense
CGAATACATCGTCACCAATTACCCA 67 253 372 Antisense
GATGCTCGATGCAACTGCTGAGGAC 609 413 513 Antisense
CTCTCTCTCGCGTGCTAGAATGGAC 492 231 585 Antisense
TGGACGCCTACAAGCAGTACGGAGC 97 549 605 Antisense
GTACGGAGCAGCCGCTGCAAACCTC 690 457 621 Antisense
CTCTTCCCATACTTCAGTCCAGGAA 347 229 643 Antisense
GTATCTGACTGCTCCAAATGGCTTT 168 453 690 Antisense
CAAATGATGCCGATTCCAGCGATGC 563 193 724 Antisense
CGATGCCTCAAGTTTTCGCACAACC 238 247 743 Antisense
Sequence Source Affymetrix Proprietary Database