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[193946_s_at] alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase

Description B0416.6 /REP_DB=WormBase Gene ID /WP=CE26434 /GB=AAG38885.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 20      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 81      6grp_total > 40      4grp_total > 55      6grp_highest_sub > 02      6grp_cgc > 72      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 397.7
(3)
P 		293.2
(-1.7)
P 		265.2
(-3)
P 		326.1
(-0.2)
P 		133
B 300.4
(0.2)
P 		290.9
(-1)
P 		276.2
(-3)
P 		321.4
(3)
P 		45
C 357.2
(3)
P 		241.2
(-1)
P 		343.5
(2.5)
P 		184
(-3)
P 		173
avr 351.8
(3) 		275.1
(-3) 		295
(-1.4) 		277.2
(-2.8) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 172
1 2 3 4 5 6 range
A 253.7
(-0.7)
P 		309.8
(1.3)
P 		358.9
(3)
P 		340.4
(2.4)
P 		186.6
(-3)
P 		226.1
(-1.6)
P 		172
B 311.4
(1.8)
P 		336.5
(3)
P 		291
(0.8)
P 		227.7
(-2.3)
P 		213.6
(-3)
P 		259.3
(-0.8)
P 		123
C 307.6
(1.1)
P 		308.8
(1.1)
P 		260
(-2.5)
P 		333.3
(3)
P 		253.9
(-3)
P 		287.3
(-0.5)
P 		79
avr 290.9
(1.4) 		318.4
(3) 		303.3
(2.1) 		300.5
(1.9) 		218
(-3) 		257.6
(-0.6) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 144.8
(3)
P 		63.6
(-2)
P 		69.3
(-1.7)
P 		47.7
(-3)
P 		97
B 95.8
(3)
P 		71.9
(-1.5)
P 		64
(-3)
P 		68.5
(-2.2)
P 		32
C 118.2
(2.9)
P 		69.3
(-0.4)
P 		119.7
(3)
P 		31
(-3)
P 		89
avr 119.6
(3) 		68.3
(-1.4) 		84.3
(-0) 		49.1
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 46.8
(-3)
P 		110.2
(3)
P 		78.2
(-0)
P 		86.3
(0.7)
P 		56.3
(-2.1)
P 		55.1
(-2.2)
P 		63
B 108.4
(2.6)
P 		112
(3)
P 		70.7
(-1.8)
P 		60.3
(-3)
P 		86.8
(0.1)
P 		96.7
(1.2)
P 		52
C 114
(3)
P 		94.2
(0.5)
P 		66.2
(-3)
P 		67
(-2.9)
P 		82.2
(-1)
P 		96.2
(0.8)
P 		48
avr 89.7
(0.2) 		105.5
(3) 		71.7
(-2.9) 		71.2
(-3) 		75.1
(-2.3) 		82.7
(-1) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193946_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16036
Sequence Type Exemplar sequence
Representative Public ID B0416.6 NCBI
Target Description B0416.6 /REP_DB=WormBase Gene ID /WP=CE26434 /GB=AAG38885.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077165(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077165 NCBI Caenorhabditis elegans GLYcosylation related family member (gly-13) (gly-13) mRNA, complete cds. 11/11 A 
SNAP00000036824 ENSEMBL cdna:SNAP chromosome:CEL140:X:9293931:9297854:-1 11/11 None
GENEFINDER00000036832 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9293931:9296522:-1 11/11 None
B0416.6.1 ENSEMBL cdna:known chromosome:CEL140:X:9293605:9296554:-1 gene:B0416.6 11/11 None
B0416.6.2 ENSEMBL cdna:known chromosome:CEL140:X:9293605:9296554:-1 gene:B0416.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9293930-9296522(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase
Gene Symbol gly-13
Ensembl B0416.6
Entrez Gene 181160 Entrez gene
SwissProt Q11068 EMBL-EBI
WormBase CE26434 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
139 Golgi membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3827 alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG38885 Glycosylation related protein 13 [Caenorhabditis elegans] ref|NP_509566.1| GLYcosylation related family member (gly-13) [Caenorhabditis elegans] sp|Q11068|MGAT1_CAEEL Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I) gb|AAD03022.1| UDP-N-acetylglucosamine:a-3-D-mannoside b-1,2-N-acetylglucosaminyltransferase I [Caenorhabditis elegans] 0.0
blast E89606 protein B0416.6 [imported] - Caenorhabditis elegans 0.0
blast CAE69842 Hypothetical protein CBG16168 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.4E-14
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 2.1E-73
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509566.1 1 7-22
B0416.6.1 1 7-22
B0416.6.2 1 7-22
GENEFINDER00000036832 1 7-22
Sequence
Target Sequence 
>C. ELEGANS:193946_S_AT
tgactgcggcttctacgttgtacagaagtgacttctttgcaggacttggctggatgatgt
cttcgaaaacgtggcatgaactagaaccaatttggcctgtaggattttgggatgactgga
tgcgagaccctgcacggcgtaaagatcgtcaatgcatccgacctgaaatcagtaggacag
ggatgatgtcttatggaaaagaaggcgccagcaaaggccagttcttcagcaagcatttgg
caaagataaaagtcaacgataagtacatcaactttgggaagattgacttggattatttgt
tgcctgccaatttcgcgaagaaaacaaatttggaagtcatgaaggaagccgttgagctga
gcatagataatgtggcttcttttgtattgagctcggagaacaaaggaaaaagtgtcagag
tgatgtacgatggcaatattgactacatccggaaagctgacaaacttcatataatgcacg
atttcaaagctggtgtcccacgtactgcatatgatggaatagtcacttgttttataaacg
gaattcgtatttacttggtacctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACTGCGGCTTCTACGTTGTACAG 315 571 737 Antisense
AGTGACTTCTTTGCAGGACTTGGCT 92 59 763 Antisense
AGAACCAATTTGGCCTGTAGGATTT 376 67 819 Antisense
GACCCTGCACGGCGTAAAGATCGTC 281 381 862 Antisense
AAGATCGTCAATGCATCCGACCTGA 376 153 878 Antisense
GGCCAGTTCTTCAGCAAGCATTTGG 122 543 952 Antisense
GGCTTCTTTTGTATTGAGCTCGGAG 613 537 1110 Antisense
GACTACATCCGGAAAGCTGACAAAC 558 373 1177 Antisense
GCACGATTTCAAAGCTGGTGTCCCA 557 315 1212 Antisense
GTGTCCCACGTACTGCATATGATGG 653 485 1229 Antisense
GGAATTCGTATTTACTTGGTACCTG 248 533 1276 Antisense
Sequence Source Affymetrix Proprietary Database