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[193933_at]

Description F45G2.2 /REP_DB=WormBase Gene ID /WP=CE16045 /TR=O62244 /GB=CAB07611.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Myosin head (motor domain) (contains ATP-GTP binding P-loop)
Cluster 4grp_high_8_8 > 33      6grp_total > 25      4grp_total > 47      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 465
1 2 3 4 range
A 459.2
(3)
P 		166.9
(-2.2)
P 		121.9
(-3)
A 		174
(-2.1)
A 		337
B 550.2
(3)
P 		157.1
(-2.1)
A 		105.4
(-2.7)
A 		85.2
(-3)
P 		465
C 531.5
(3)
P 		179
(-2.2)
A 		122.5
(-3)
P 		160.6
(-2.4)
A 		409
avr 513.6
(3) 		167.7
(-2.2) 		116.6
(-3) 		139.9
(-2.6) 		397

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 320
1 2 3 4 5 6 range
A 115.6
(-2)
P 		338.8
(3)
P 		137.9
(-1.5)
A 		136.6
(-1.5)
P 		87.3
(-2.7)
A 		72.1
(-3)
A 		267
B 271.9
(3)
P 		197.8
(1)
P 		143.2
(-0.4)
M 		101.7
(-1.5)
P 		52.4
(-2.9)
A 		47.4
(-3)
A 		225
C 335.7
(3)
P 		190.4
(0.2)
P 		211.3
(0.6)
P 		18.7
(-3)
A 		64.4
(-2.1)
A 		83.8
(-1.8)
A 		317
avr 241.1
(3) 		242.3
(3) 		164.1
(0.3) 		85.7
(-2.4) 		68
(-3) 		67.8
(-3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 167.2
(3)
P 		36.2
(-2.5)
P 		31.9
(-2.7)
A 		25.5
(-3)
A 		142
B 175.5
(3)
P 		38.8
(-2.2)
A 		24.4
(-2.8)
A 		18.2
(-3)
P 		157
C 175.9
(3)
P 		51.5
(-2)
A 		42.7
(-2.4)
P 		27.1
(-3)
A 		149
avr 172.9
(3) 		42.2
(-2.3) 		33
(-2.6) 		23.6
(-3) 		149

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 21.3
(-2.8)
P 		120.5
(3)
P 		30
(-2.3)
A 		34.6
(-2)
P 		26.3
(-2.5)
A 		17.5
(-3)
A 		103
B 94.6
(3)
P 		65.8
(0.8)
P 		34.8
(-1.7)
M 		26.9
(-2.3)
P 		21.3
(-2.7)
A 		17.7
(-3)
A 		77
C 124.4
(3)
P 		58.1
(-0.3)
P 		53.8
(-0.5)
P 		3.8
(-3)
A 		20.8
(-2.2)
A 		28
(-1.8)
A 		121
avr 80.1
(2.9) 		81.5
(3) 		39.5
(-1.2) 		21.8
(-2.9) 		22.8
(-2.8) 		21.1
(-3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193933_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7131
Sequence Type Exemplar sequence
Representative Public ID F45G2.2 NCBI
Target Description F45G2.2 /REP_DB=WormBase Gene ID /WP=CE16045 /TR=O62244 /GB=CAB07611.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Myosin head (motor domain) (contains ATP-GTP binding P-loop)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027419(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027419 NCBI Caenorhabditis elegans F45G2.2a (F45G2.2) mRNA, complete cds. 10/11 None
SNAP00000011665 ENSEMBL cdna:SNAP chromosome:CEL140:III:13409459:13414216:1 10/11 None
GENEFINDER00000011686 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13409459:13414216:1 10/11 None
F45G2.2a ENSEMBL cdna:known chromosome:CEL140:III:13409437:13423042:1 gene:F45G2.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027420 6/11 Cross Hyb Matching Probes None
F45G2.2b.2 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176764 2 1 NM_001027419 174619_at (11), 193933_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13409454-13414419(+) 100.0 100.0
Public Domain and Genome References
Ensembl F45G2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07611 Hypothetical protein F45G2.2a [Caenorhabditis elegans] ref|NP_001022590.1| F45G2.2a [Caenorhabditis elegans] 0.0
blast CAE73701 Hypothetical protein CBG21212 [Caenorhabditis briggsae] 0.0
blast AAG24132 Hypothetical protein F58G4.1 [Caenorhabditis elegans] ref|NP_505094.1| F58G4.1 [Caenorhabditis elegans] 0.0
blast AAA73080 [Brugia malayi myosin heavy chain gene, complete cds.], gene product 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 4.5E-14
Pfam IPR002928 EMBL-EBI Myosin tail 5.4E-14
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193933_AT
gctcacttctcaattatgcactacgctggaacagttcgatacaatgctgaacagtggttg
gataagaataaggatccattgaatgattcggcagttgctatattgaagacaagtgataag
gaaggtgtgctttatcagttatgggaggaatatcaaacagatgttgatagagaggaaagt
gagaaaagaggaaaagctcaggcaaagaagaagggaaaatctgcatcgttccttactgtt
tcgactatgtacagagaatcattgacttctttgatgactatgcttcacacaacacatcct
cattttatcagatgtatcattccaaacgagaagaagacatctggattaatcgatgctcca
ttggttctcaatcagctcacgtgcaacggtgtgctggaaggaattcgaatttgcagaaaa
ggattccccaatagaatgatgttcgccgatttccgattcagatatgctatccttgcagct
gatgaagcttctgataaggatgcggctaaggcttcaaaggcaatgctccgtaagttgagc
aagaataatcagctgaatgtggatactttcaaagttggaaccacgaagtttcggctcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCACTTCTCAATTATGCACTACG 594 301 1720 Antisense
ATGCACTACGCTGGAACAGTTCGAT 467 43 1735 Antisense
CATCGTTCCTTACTGTTTCGACTAT 624 211 1943 Antisense
TTCACACAACACATCCTCATTTTAT 279 687 2003 Antisense
ATTAATCGATGCTCCATTGGTTCTC 593 11 2064 Antisense
CATTGGTTCTCAATCAGCTCACGTG 662 217 2078 Antisense
AGCTCACGTGCAACGGTGTGCTGGA 494 79 2093 Antisense
CGCCGATTTCCGATTCAGATATGCT 258 257 2163 Antisense
GATATGCTATCCTTGCAGCTGATGA 610 423 2180 Antisense
AAGGCTTCAAAGGCAATGCTCCGTA 371 161 2227 Antisense
TGGAACCACGAAGTTTCGGCTCGAT 58 547 2295 Antisense
Sequence Source Affymetrix Proprietary Database