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[193932_s_at] DnaJ, prokaryotic heat shock protein, Zinc finger, C2H2 type

Description T03F6.2 /REP_DB=WormBase Gene ID /WP=CE16336 /TR=O62360 /GB=CAB03279.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DnaJ, prokaryotic heat shock protein, Zinc finger, C2H2 type
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 75      4grp_highest_8_8 > 26      4grp_high_8_8 > 60      6grp_highest_nonorm > 83      6grp_total > 66      4grp_total > 60      6grp_highest_sub > Da      6grp_cgc > 47      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 296
1 2 3 4 range
A 413.7
(0.8)
P 		465
(2.3)
P 		488.6
(3)
P 		280.5
(-3)
P 		208
B 440.4
(0.8)
P 		435.4
(0.7)
P 		532.1
(3)
P 		281.5
(-3)
P 		251
C 473.5
(-0.6)
P 		405.4
(-3)
P 		576.6
(3)
P 		412.6
(-2.7)
P 		171
avr 442.5
(0.4) 		435.3
(0.2) 		532.4
(3) 		324.9
(-3) 		208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 400
1 2 3 4 5 6 range
A 332.6
(-2)
P 		424.2
(-0.1)
P 		361.9
(-1.4)
P 		401.9
(-0.6)
P 		285
(-3)
P 		572.5
(3)
P 		288
B 325.9
(-3)
P 		366.5
(-2.1)
P 		434.8
(-0.5)
P 		377.8
(-1.8)
P 		378.9
(-1.8)
P 		590.9
(3)
P 		265
C 421
(-1.9)
P 		360.3
(-3)
P 		495.3
(-0.5)
P 		444.1
(-1.5)
P 		362.7
(-3)
P 		684.8
(3)
P 		325
avr 359.8
(-2.6) 		383.7
(-2.1) 		430.7
(-1.1) 		407.9
(-1.6) 		342.2
(-3) 		616.1
(3) 		274
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 150.6
(3)
P 		100.9
(0.3)
P 		127.7
(1.7)
P 		41.1
(-3)
P 		110
B 140.4
(3)
P 		107.5
(0.5)
P 		123.3
(1.7)
P 		60
(-3)
P 		80
C 156.7
(1)
P 		116.6
(-0.9)
P 		201
(3)
P 		69.6
(-3)
P 		131
avr 149.2
(2.9) 		108.3
(0.3) 		150.7
(3) 		56.9
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 61.3
(-3)
P 		150.9
(3)
P 		78.8
(-1.8)
P 		101.9
(-0.3)
P 		86
(-1.3)
P 		139.4
(2.2)
P 		90
B 113.4
(-2.3)
P 		122
(-1.9)
P 		105.6
(-2.7)
P 		100.1
(-3)
P 		153.9
(-0.3)
P 		220.3
(3)
P 		120
C 156
(-0.1)
P 		109.9
(-2.1)
P 		126.1
(-1.4)
P 		89.3
(-3)
P 		117.4
(-1.8)
P 		229.3
(3)
P 		140
avr 110.2
(-2.2) 		127.6
(-1.2) 		103.5
(-2.6) 		97.1
(-3) 		119.1
(-1.7) 		196.3
(3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193932_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6096
Sequence Type Exemplar sequence
Representative Public ID T03F6.2 NCBI
Target Description T03F6.2 /REP_DB=WormBase Gene ID /WP=CE16336 /TR=O62360 /GB=CAB03279.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DnaJ, prokaryotic heat shock protein, Zinc finger, C2H2 type
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067358(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067358 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-17) (dnj-17) mRNA, complete cds. 11/11 None
SNAP00000014317 ENSEMBL cdna:SNAP chromosome:CEL140:III:13390441:13392851:-1 11/11 None
GENEFINDER00000014321 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13390441:13392851:-1 11/11 None
T03F6.2 ENSEMBL cdna:known chromosome:CEL140:III:13390439:13393397:-1 gene:T03F6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13390436-13393393(-) 100.0 100.0
Public Domain and Genome References
Gene Title DnaJ, prokaryotic heat shock protein, Zinc finger, C2H2 type
Gene Symbol dnj-17
Ensembl T03F6.2
Entrez Gene 176761 Entrez gene
SwissProt O62360 EMBL-EBI
WormBase CE16336 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260251_AT DNAJ heat shock N-terminal domain-containing protein at
CANINE:1592021_AT similar to DnaJ homology subfamily A member 5 isoform 2 cfa
CANINE:1589417_S_AT similar to DnaJ homology subfamily A member 5 isoform 2 cfa
CANINE_2:CFA.11088.1.A1_S_AT similar to DnaJ homology subfamily A member 5 isoform 2 cfa
CANINE_2:CFAAFFX.28709.1.S1_AT similar to DnaJ homology subfamily A member 5 isoform 2 cfa
DROSOPHILA_2:1626565_AT dm
DROSGENOME1:151900_AT dm
CHICKEN:GGA.11790.1.S1_AT similar to hypothetical protein LOC134218 gga
CHICKEN:GGAAFFX.25833.1.S1_AT similar to hypothetical protein LOC134218 gga
HG-U133_PLUS_2:234094_X_AT DnaJ homology subfamily A member 5 hs
HG-U133B:234094_X_AT DnaJ homology subfamily A member 5 hs
HU35KSUBA:RC_AA453437_AT DnaJ homology subfamily A member 5 hs
HU35KSUBA:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HU35KSUBB:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HU35KSUBC:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HU35KSUBD:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HUGENEFL:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HC-G110:HUM_ALU_AT DnaJ homology subfamily A member 5 hs
HU35KSUBC:RC_AA159825_AT DnaJ homology subfamily A member 5 hs
HU35KSUBD:RC_AA599920_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.34359.0.A1_3P_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.296725.0.S1_3P_X_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.296725.0.S1_3P_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.131887.2.A1_3P_X_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.131887.2.A1_3P_A_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.131887.1.A1_3P_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.131887.0.A1_3P_AT DnaJ homology subfamily A member 5 hs
U133_X3P:234094_3P_X_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:230893_AT DnaJ homology subfamily A member 5 hs
HG-U133B:230893_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:235001_AT DnaJ homology subfamily A member 5 hs
HG-U133B:235001_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:235032_AT DnaJ homology subfamily A member 5 hs
HG-U133B:235032_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:238337_S_AT DnaJ homology subfamily A member 5 hs
HG-U133B:238337_S_AT DnaJ homology subfamily A member 5 hs
HG-U95C:61713_AT DnaJ homology subfamily A member 5 hs
HG-U95C:61916_AT DnaJ homology subfamily A member 5 hs
HG-U95D:73791_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS2.301470.1.S1_3P_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:1556375_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:238335_AT DnaJ homology subfamily A member 5 hs
HG-U133B:238335_AT DnaJ homology subfamily A member 5 hs
HG-U133_PLUS_2:238336_S_AT DnaJ homology subfamily A member 5 hs
HG-U133B:238336_S_AT DnaJ homology subfamily A member 5 hs
U133_X3P:HS.131887.2.S1_3P_AT DnaJ homology subfamily A member 5 hs
MOUSE430A_2:1460711_AT RIKEN cDNA 4930461P20 gene mm
MOE430A:1460711_AT RIKEN cDNA 4930461P20 gene mm
MOUSE430_2:1460711_AT RIKEN cDNA 4930461P20 gene mm
MU11KSUBA:AA681894_AT RIKEN cDNA 4930461P20 gene mm
MU11KSUBA:AA681894_G_AT RIKEN cDNA 4930461P20 gene mm
MG-U74AV2:93478_AT RIKEN cDNA 4930461P20 gene mm
MG-U74BV2:111970_AT RIKEN cDNA 4930461P20 gene mm
MG-U74CV2:169515_R_AT RIKEN cDNA 4930461P20 gene mm
MOE430B:1457281_AT RIKEN cDNA 4930461P20 gene mm
MOUSE430_2:1457281_AT RIKEN cDNA 4930461P20 gene mm
MOE430B:1420220_X_AT RIKEN cDNA 4930461P20 gene mm
MOUSE430_2:1420220_X_AT RIKEN cDNA 4930461P20 gene mm
MOE430B:1420219_AT RIKEN cDNA 4930461P20 gene mm
MOUSE430_2:1420219_AT RIKEN cDNA 4930461P20 gene mm
MU19KSUBA:TC25691_AT PREDICTED: RIKEN cDNA 4930461P20 [Mus musculus], mRNA sequence mm
RG-U34B:RC_AA850140_AT putative regulation protein GS3 rn
RAE230A:1369543_S_AT putative regulation protein GS3 rn
RAT230_2:1369543_S_AT putative regulation protein GS3 rn
YEAST_2:1777481_AT Protein that contains a 70 amino acid J-domain, may function as a co-chaperone to recruit Hsp70-like activity to specific sites; mutation causes defects in fluid-phase endocytosis Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03279 Hypothetical protein T03F6.2 [Caenorhabditis elegans] ref|NP_499759.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-17) [Caenorhabditis elegans] 0.0
blast CAE62857 Hypothetical protein CBG07036 [Caenorhabditis briggsae] 1.0E-170
blast XP_536503 PREDICTED: similar to DnaJ homology subfamily A member 5 isoform 2 isoform 1 [Canis familiaris] 2.0E-84
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 7.99999999855967E-23
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 5.00000015687197E-23
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.0E-32
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.0E-32
Sequence
Target Sequence 
>C. ELEGANS:193932_S_AT
acttccatattgtgttgtttgtagcaaatcgttcaaaactgtaaacgccaagttgaatca
tgagaactcgaagcaacacatccgtcagctgaatgagctgaaaaagcatatgaaagaaga
agattccacgattttcaccgaagcagaaccgaaacccgacgaaacaccgaaaggaaagaa
gaataagagaaaggataggaaaaaggggaatggaatatttgatgtggaagaaacagaagc
agtagaagccgagattcaggaagcgccgcccgtggaaaatgagcagccggaggaagagga
aaaggaatgtaccccggaagttgttaagaaagagaaaaagaagagaagagctgataaaaa
tcaaaacccatcggcgacagtagaggaagaagcaccgaagtcaactgaaccaaaatctgc
aatttgtgataaatgccgtgaggtattcgatagccggaccaggttatttgctcatttaca
agaaaccggacacgccacgttgctcatcaacaaaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTCCATATTGTGTTGTTTGTAGC 2 105 954 Antisense
GAGAACTCGAAGCAACACATCCGTC 680 391 1015 Antisense
GCAACACATCCGTCAGCTGAATGAG 57 319 1026 Antisense
AAGATTCCACGATTTTCACCGAAGC 58 157 1073 Antisense
TCACCGAAGCAGAACCGAAACCCGA 438 621 1088 Antisense
AAATCAAAACCCATCGGCGACAGTA 377 113 1311 Antisense
GAAGCACCGAAGTCAACTGAACCAA 633 341 1342 Antisense
GAGGTATTCGATAGCCGGACCAGGT 175 403 1393 Antisense
GGACCAGGTTATTTGCTCATTTACA 470 523 1409 Antisense
TTACAAGAAACCGGACACGCCACGT 551 599 1429 Antisense
GCCACGTTGCTCATCAACAAACCAG 288 279 1447 Antisense
Sequence Source Affymetrix Proprietary Database