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[193929_s_at] gamma-butyrobetaine,2-oxoglutarate dioxygenase

Description D2089.5 /REP_DB=WormBase Gene ID /WP=CE00940 /GEN=gbh-1 /TR=SW:Q19000 /GB=CAA85412.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=gamma-butyrobetaine,2-oxoglutarate dioxygenase
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 66      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 84      6grp_total > 67      4grp_total > 60      6grp_highest_sub > Cu      6grp_cgc > 47      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 617
1 2 3 4 range
A 700.3
(1)
P 		585.1
(-0.5)
P 		856.4
(3)
P 		394.3
(-3)
P 		462
B 698.9
(1)
P 		771.3
(3)
P 		654.8
(-0.3)
P 		557.7
(-3)
P 		214
C 799.6
(0.8)
P 		627.1
(-1)
P 		1011.2
(3)
P 		436.2
(-3)
P 		575
avr 732.9
(1.3) 		661.2
(0.1) 		840.8
(3) 		462.7
(-3) 		378

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 578
1 2 3 4 5 6 range
A 533
(-0.8)
P 		384
(-3)
P 		688.3
(1.5)
P 		513.4
(-1.1)
P 		550.8
(-0.5)
P 		787.1
(3)
P 		403
B 800.8
(1.2)
P 		409.7
(-3)
P 		690
(0)
P 		455.8
(-2.5)
P 		558.9
(-1.4)
P 		962.4
(3)
P 		553
C 510.7
(-1.7)
P 		476.7
(-2.3)
P 		479.2
(-2.2)
P 		451.3
(-2.6)
P 		427.2
(-3)
P 		824.7
(3)
P 		398
avr 614.8
(-0.4) 		423.5
(-3) 		619.2
(-0.3) 		473.5
(-2.3) 		512.3
(-1.8) 		858.1
(3) 		435
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 255
(3)
P 		127
(-0.9)
P 		223.9
(2.1)
P 		57.7
(-3)
P 		197
B 222.9
(3)
P 		190.5
(1.1)
P 		151.7
(-1.1)
P 		118.8
(-3)
P 		104
C 264.7
(1.1)
P 		180.3
(-0.7)
P 		352.4
(3)
P 		73.5
(-3)
P 		279
avr 247.5
(3) 		165.9
(0) 		242.7
(2.8) 		83.3
(-3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 98.3
(-3)
P 		136.6
(-0.5)
P 		149.9
(0.3)
P 		130.2
(-0.9)
P 		166.2
(1.4)
P 		191.6
(3)
P 		93
B 278.6
(1)
P 		136.4
(-2.6)
P 		167.6
(-1.8)
P 		120.7
(-3)
P 		227.1
(-0.3)
P 		358.9
(3)
P 		238
C 189.3
(0.2)
P 		145.5
(-1.2)
P 		122
(-2)
P 		90.8
(-3)
P 		138.2
(-1.5)
P 		276.1
(3)
P 		185
avr 188.7
(-0.2) 		139.5
(-2) 		146.5
(-1.8) 		113.9
(-3) 		177.2
(-0.6) 		275.5
(3) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193929_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3597
Sequence Type Exemplar sequence
Representative Public ID D2089.5 NCBI
Target Description D2089.5 /REP_DB=WormBase Gene ID /WP=CE00940 /GEN=gbh-1 /TR=SW:Q19000 /GB=CAA85412.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=gamma-butyrobetaine,2-oxoglutarate dioxygenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063904(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063904 NCBI Caenorhabditis elegans Gamma Butyrobetaine Hydroxylase family member (gbh-1) (gbh-1) mRNA, complete cds. 11/11 None
SNAP00000043967 ENSEMBL cdna:SNAP chromosome:CEL140:II:10690437:10692506:-1 11/11 None
GENEFINDER00000043970 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10690437:10693072:-1 11/11 None
D2089.5 ENSEMBL cdna:known chromosome:CEL140:II:10690387:10693716:-1 gene:D2089.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10690433-10693695(-) 100.0 100.0
Public Domain and Genome References
Gene Title gamma-butyrobetaine,2-oxoglutarate dioxygenase
Gene Symbol gbh-1
Ensembl D2089.5
Entrez Gene 174648 Entrez gene
SwissProt Q19000 EMBL-EBI
WormBase CE00940 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597058_AT similar to butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) cfa
CANINE:1590693_S_AT similar to butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) cfa
CANINE_2:CFA.9989.1.A1_AT similar to butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) cfa
CANINE_2:CFAAFFX.15922.1.S1_AT similar to butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) cfa
DROSOPHILA_2:1638582_AT dm
DROSGENOME1:145074_AT dm
CHICKEN:GGA.12310.1.S1_AT similar to gamma butyrobetaine hydroxylase gga
HU35KSUBA:RC_AA455988_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HU35KSUBD:RC_F10380_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-U133A:205363_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-U133_PLUS_2:205363_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-FOCUS:205363_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-U133A_2:205363_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
U133_X3P:G4502368_3P_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-U95AV2:38339_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
HG-U95D:79797_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 hs
MOUSE430_2:1419618_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) mm
MOE430A:1419618_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) mm
MOUSE430A_2:1419618_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) mm
MG-U74BV2:111482_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) mm
MU19KSUBB:TC29809_AT Butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) (Bbox1), mRNA mm
MU19KSUBB:TC32319_AT Butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) (Bbox1), mRNA mm
RAE230A:1369546_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) rn
RAT230_2:1369546_AT butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) rn
RAE230B:1391293_AT Butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) rn
RAT230_2:1391293_AT Butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) rn
RG-U34C:RC_AI170038_AT Butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1 (gamma-butyrobetaine hydroxylase) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85412 Hypothetical protein D2089.5 [Caenorhabditis elegans] ref|NP_496305.1| Gamma Butyrobetaine Hydroxylase family member (gbh-1) [Caenorhabditis elegans] sp|Q19000|BODG_CAEEL Probable gamma-butyrobetaine dioxygenase (Gamma-butyrobetaine,2-oxoglutarate dioxygenase) (Gamma-butyrobetaine hydroxylase) (Gamma-BBH) 0.0
blast CAE59623 Hypothetical protein CBG03032 [Caenorhabditis briggsae] 0.0
blast AAM82167 gamma-butyrobetaine,2-oxoglutarate dioxygenase; GAMMA-BBH [Angiostrongylus cantonensis] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR004994 EMBL-EBI Gamma-butyrobetaine hydroxylase 1.0E-126
Pfam IPR004994 EMBL-EBI Gamma-butyrobetaine hydroxylase 1.0E-126
Pfam IPR004994 EMBL-EBI Gamma-butyrobetaine hydroxylase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193929_S_AT
gggactaccatttcacacggattttccgtctttgtcacaccctccacagctccaaatgct
ccatatgctacaaagtgcggaagaaggaggacatagtctatttgttgatggatttcatgt
cgcagagcagctccgagttgaaaagccagagatcttcaaaatcttgaccactcaatccat
ggaatacattgaagaaggatatgatgttcatgagattaatggaaaaactatacgctttga
ttatgacatgtgtgcaaggcacaaggttattcggctgaatgatgacggaaaagtcaataa
gatacagttcggaaacgccatgcgcagttggttctacgattgtgagccaagcaaggttca
ggatgtttatagagcaatgaaaacattcaccgaatactgctatcaaccacgcaatatgct
gaaattccggcttgaagacggtgacaccgttttatgggctaatcaacgattattacacac
tcgagatggattccgaaatgccccagaaaaggccagaacactgacgggttgctatttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGACTACCATTTCACACGGATTTT 559 493 663 Antisense
CACGGATTTTCCGTCTTTGTCACAC 48 199 678 Antisense
GGATTTCATGTCGCAGAGCAGCTCC 70 511 772 Antisense
AAATCTTGACCACTCAATCCATGGA 592 115 821 Antisense
GCAAGGCACAAGGTTATTCGGCTGA 382 321 916 Antisense
GATACAGTTCGGAAACGCCATGCGC 315 423 963 Antisense
CGCCATGCGCAGTTGGTTCTACGAT 650 257 978 Antisense
TTCACCGAATACTGCTATCAACCAC 237 687 1048 Antisense
GGTGACACCGTTTTATGGGCTAATC 428 499 1102 Antisense
AGATGGATTCCGAAATGCCCCAGAA 566 67 1146 Antisense
GAACACTGACGGGTTGCTATTTCGA 66 351 1178 Antisense
Sequence Source Affymetrix Proprietary Database