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[193921_s_at] N2,N2-dimethylguanosine tRNA methyltransferase

Description ZC376.5 /REP_DB=WormBase Gene ID /WP=CE15201 /GEN=trm-1 /TR=SW:Q23270 /GB=CAB00881.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=N2,N2-dimethylguanosine tRNA methyltransferase
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 33      4grp_high_8_8 > 51      6grp_highest_nonorm > 73      6grp_total > 78      4grp_total > 61      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 622.3
(3)
P 		602.6
(2.6)
P 		619.9
(3)
P 		303.7
(-3)
P 		319
B 711.5
(3)
P 		599
(1.3)
P 		646.3
(2)
P 		317.2
(-3)
P 		394
C 656.5
(3)
P 		656
(3)
P 		653.7
(3)
P 		280.8
(-3)
P 		376
avr 663.4
(3) 		619.2
(2.3) 		640
(2.6) 		300.6
(-3) 		363

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 403.6
(-3)
P 		561.1
(1)
P 		589
(1.7)
P 		468.1
(-1.4)
P 		571.3
(1.3)
P 		639.4
(3)
P 		236
B 554.3
(-1)
P 		521.5
(-2.2)
P 		500.6
(-2.9)
P 		497.2
(-3)
P 		553.1
(-1.1)
P 		671
(3)
P 		174
C 446.5
(-2.9)
P 		509.7
(-1.3)
P 		529.5
(-0.9)
P 		442.9
(-3)
P 		532.6
(-0.8)
P 		685.2
(3)
P 		242
avr 468.1
(-3) 		530.8
(-1.1) 		539.7
(-0.8) 		469.4
(-3) 		552.3
(-0.4) 		665.2
(3) 		197
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 226.6
(3)
P 		130.8
(-0.2)
P 		162.1
(0.9)
P 		44.5
(-3)
P 		182
B 226.9
(3)
P 		147.9
(0)
P 		149.8
(0.1)
P 		67.6
(-3)
P 		159
C 217.3
(2.7)
P 		188.6
(1.7)
P 		227.8
(3)
P 		47.3
(-3)
P 		181
avr 223.6
(3) 		155.8
(0.6) 		179.9
(1.5) 		53.1
(-3) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 74.4
(-3)
P 		199.5
(3)
P 		128.3
(-0.4)
P 		118.7
(-0.9)
P 		172.4
(1.7)
P 		155.7
(0.9)
P 		125
B 192.9
(0.3)
P 		173.6
(-0.6)
P 		121.6
(-3)
P 		131.7
(-2.5)
P 		224.7
(1.8)
P 		250.2
(3)
P 		129
C 165.5
(0.3)
P 		155.5
(-0.2)
P 		134.8
(-1)
P 		89.1
(-3)
P 		172.3
(0.6)
P 		229.4
(3)
P 		140
avr 144.3
(-1.1) 		176.2
(0.8) 		128.2
(-2.1) 		113.2
(-3) 		189.8
(1.7) 		211.8
(3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193921_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14640
Sequence Type Exemplar sequence
Representative Public ID ZC376.5 NCBI
Target Description ZC376.5 /REP_DB=WormBase Gene ID /WP=CE15201 /GEN=trm-1 /TR=SW:Q23270 /GB=CAB00881.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=N2,N2-dimethylguanosine tRNA methyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074112(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074112 NCBI Caenorhabditis elegans TRNA Methylase family member (trm-1) (trm-1) mRNA, complete cds. 11/11 None
SNAP00000015358 ENSEMBL cdna:SNAP chromosome:CEL140:V:14185814:14188057:-1 11/11 None
GENEFINDER00000015361 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14184152:14188057:-1 11/11 None
ZC376.5 ENSEMBL cdna:known chromosome:CEL140:V:14185712:14188209:-1 gene:ZC376.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14185813-14188057(-) 99.68 100.0
Public Domain and Genome References
Gene Title N2,N2-dimethylguanosine tRNA methyltransferase
Gene Symbol trm-1
Ensembl ZC376.5
Entrez Gene 179920 Entrez gene
SwissProt Q23270 EMBL-EBI
WormBase CE15201 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246521_AT N2,N2-dimethylguanosine tRNA methyltransferase family protein at
CANINE:1585692_AT similar to Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) cfa
CANINE_2:CFA.11348.1.A1_S_AT similar to Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) cfa
CANINE_2:CFAAFFX.26112.1.S1_AT similar to Probable N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) cfa
DROSOPHILA_2:1636003_AT dm
DROSGENOME1:154663_AT dm
HG-U133A:203701_S_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:203701_S_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133A_2:203701_S_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:210463_X_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133A:210463_X_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133A_2:210463_X_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
U133_X3P:G12803346_3P_A_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U95AV2:32096_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U95D:71211_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:216454_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133A_2:216454_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
HG-U133A:216454_AT TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) hs
MOUSE430A_2:1437314_A_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOE430A:1437314_A_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOUSE430_2:1437314_A_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MU11KSUBA:AA103548_S_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MU11KSUBB:MSA.33517.0_S_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MG-U74AV2:99656_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MG-U74CV2:130627_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOE430A:1451249_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOUSE430A_2:1451249_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOUSE430_2:1451249_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOE430A:1436494_X_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOUSE430A_2:1436494_X_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MOUSE430_2:1436494_X_AT DNA segment, Chr 8, ERATO Doi 812, expressed mm
MU19KSUBA:TC24393_AT DNA segment, Chr 8, ERATO Doi 812, expressed, mRNA (cDNA clone MGC:37065 IMAGE:4950980) mm
MU19KSUBB:TC31027_AT DNA segment, Chr 8, ERATO Doi 812, expressed, mRNA (cDNA clone MGC:37065 IMAGE:4950980) mm
MU19KSUBC:TC41316_AT DNA segment, Chr 8, ERATO Doi 812, expressed, mRNA (cDNA clone MGC:37065 IMAGE:4950980) mm
MU19KSUBC:TC41316_G_AT DNA segment, Chr 8, ERATO Doi 812, expressed, mRNA (cDNA clone MGC:37065 IMAGE:4950980) mm
RG-U34B:RC_AI012114_AT similar to D8Ertd812e protein (predicted) rn
RAE230A:1389056_AT similar to D8Ertd812e protein (predicted) rn
RAT230_2:1389056_AT similar to D8Ertd812e protein (predicted) rn
YEAST_2:1775856_AT tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
8033 tRNA processing inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
4809 tRNA (guanine-N2-)-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00881 Hypothetical protein ZC376.5 [Caenorhabditis elegans] ref|NP_506513.1| TRNA Methylase family member (trm-1) [Caenorhabditis elegans] sp|Q23270|TRM1_CAEEL N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) gb|AAD13737.1| N2,N2-dimethylguanosine tRNA methyltransferase [Caenorhabditis elegans] 0.0
blast CAE61005 Hypothetical protein CBG04743 [Caenorhabditis briggsae] 0.0
blast CAB00880 Hypothetical protein ZC376.1 [Caenorhabditis elegans] ref|NP_506510.1| ZC376.1 [Caenorhabditis elegans] 1.0E-103
blast EAA14208 ENSANGP00000010185 [Anopheles gambiae str. PEST] ref|XP_318825.2| ENSANGP00000010185 [Anopheles gambiae str. PEST] 2.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR002905 EMBL-EBI N2,N2-dimethylguanosine tRNA methyltransferase 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 4.3E-58
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000015361 1 528-550
Sequence
Target Sequence 
>C. ELEGANS:193921_S_AT
tcgatgcatccattgcttacttccactccaccaaattggtccaatttatcttgctccaat
tcacagcaaaccgttcgtcacttcccttctggaacgtctgaaatctactcctgaagctga
aagacttggaactcacggacgtcttcaaggtgttcttacgatggtcaatgaagaactgga
tgatgttttatattatgagcacaaccaaatggcgaatgttgtgaaagtttccgttccaaa
atctcaaagtgtgagatctgcgattttaaatgccggtttcaaagtttctggatctcactg
taatccccgtgcaatcaaaacaaatgctccgatgcacttgctatgggatatttatagaca
agttgctaaagacacaagtgtggatcgcgagaagcgtctggccaaagaatcagcaggata
tcacattctcggacaacccatcaccaacacagtcaacttcactcttcacccaggagccat
cgaacaggccaagaaagagaaccttgtacggttccaatgcaataaagggaagaattgggg
ccctcgccagaaagcaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATGCATCCATTGCTTACTTCCA 481 603 981 Antisense
TCCAATTCACAGCAAACCGTTCGTC 467 587 1035 Antisense
TCACTTCCCTTCTGGAACGTCTGAA 613 621 1058 Antisense
ACGTCTGAAATCTACTCCTGAAGCT 407 93 1074 Antisense
GAACTCACGGACGTCTTCAAGGTGT 196 349 1109 Antisense
TTCTGGATCTCACTGTAATCCCCGT 513 693 1266 Antisense
AATGCTCCGATGCACTTGCTATGGG 710 165 1303 Antisense
GTGGATCGCGAGAAGCGTCTGGCCA 463 489 1360 Antisense
GCAGGATATCACATTCTCGGACAAC 611 311 1393 Antisense
CACCAACACAGTCAACTTCACTCTT 679 201 1422 Antisense
TTGGGGCCCTCGCCAGAAAGCAAAA 282 665 1515 Antisense
Sequence Source Affymetrix Proprietary Database