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[193920_at] adenylyl cyclase

Description C44F1.5 /REP_DB=WormBase Gene ID /WP=CE08721 /TR=Q18628 /GB=CAA88885.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Adenylate and Guanylate cyclase catalytic domain (2 domains)
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 23      4grp_highest_8_8 > 49      4grp_high_8_8 > 42      6grp_highest_nonorm > 71      6grp_total > 22      4grp_total > 54      6grp_highest_sub > 02      6grp_cgc > 61      6grp_high1_sub > 02      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 365
1 2 3 4 range
A 579.3
(3)
P 		359.7
(-0.8)
P 		338
(-1.1)
P 		228.6
(-3)
P 		351
B 474.5
(3)
P 		420.7
(1.5)
P 		315.8
(-1.5)
P 		262
(-3)
P 		213
C 535
(3)
P 		364.3
(-0.2)
P 		390
(0.3)
P 		214.5
(-3)
P 		321
avr 529.6
(3) 		381.6
(-0) 		347.9
(-0.7) 		235
(-3) 		295

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 378
1 2 3 4 5 6 range
A 265.4
(-1.1)
P 		519
(3)
P 		329.1
(-0.1)
P 		406
(1.2)
P 		243.4
(-1.5)
P 		150.3
(-3)
P 		369
B 438.9
(2.8)
P 		450.4
(3)
P 		368.8
(1.3)
P 		322.9
(0.4)
P 		250.3
(-1.1)
P 		158.7
(-3)
P 		292
C 442.5
(2.8)
P 		405.4
(2.1)
P 		450.7
(3)
P 		400.2
(2)
P 		263.7
(-0.6)
P 		140.6
(-3)
P 		310
avr 382.3
(1.5) 		458.3
(3) 		382.9
(1.5) 		376.4
(1.4) 		252.5
(-1) 		149.9
(-3) 		308
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 210.9
(3)
P 		78.1
(-1.5)
P 		88.4
(-1.1)
P 		33.5
(-3)
P 		177
B 151.3
(3)
P 		103.9
(0)
P 		73.2
(-1.9)
P 		55.8
(-3)
P 		96
C 177.1
(3)
P 		104.7
(-0.1)
P 		135.9
(1.2)
P 		36.2
(-3)
P 		141
avr 179.8
(3) 		95.6
(-0.7) 		99.2
(-0.5) 		41.8
(-3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 48.9
(-2.5)
P 		184.6
(3)
P 		71.7
(-1.6)
P 		103
(-0.3)
P 		73.4
(-1.5)
P 		36.6
(-3)
P 		148
B 152.7
(3)
P 		149.9
(2.8)
P 		89.5
(-1.1)
P 		85.5
(-1.3)
P 		101.7
(-0.3)
P 		59.2
(-3)
P 		94
C 164
(3)
P 		123.7
(0.9)
P 		114.7
(0.5)
P 		80.5
(-1.3)
P 		85.3
(-1)
P 		47.1
(-3)
P 		117
avr 121.9
(1.2) 		152.7
(3) 		92
(-0.5) 		89.7
(-0.6) 		86.8
(-0.8) 		47.6
(-3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193920_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6710
Sequence Type Exemplar sequence
Representative Public ID C44F1.5 NCBI
Target Description C44F1.5 /REP_DB=WormBase Gene ID /WP=CE08721 /TR=Q18628 /GB=CAA88885.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Adenylate and Guanylate cyclase catalytic domain (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065364(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065364 NCBI Caenorhabditis elegans Adenylyl CYclase family member (acy-3) (acy-3) mRNA, complete cds. 11/11 None
SNAP00000024034 ENSEMBL cdna:SNAP chromosome:CEL140:III:3775149:3779732:-1 11/11 None
GENEFINDER00000024037 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3775149:3779761:-1 11/11 None
C44F1.5 ENSEMBL cdna:known chromosome:CEL140:III:3774871:3781875:-1 gene:C44F1.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3777379-3781875(-) 100.0 100.0
Public Domain and Genome References
Gene Title adenylyl cyclase
Gene Symbol acy-3
Ensembl C44F1.5
Entrez Gene 175489 Entrez gene
SwissProt Q18628 EMBL-EBI
WormBase CE33567 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5886 plasma membrane inferred from curator QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4016 adenylate cyclase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88853 Hypothetical protein C44F1.5 [Caenorhabditis elegans] emb|CAA88885.2| Hypothetical protein C44F1.5 [Caenorhabditis elegans] ref|NP_497765.2| Adenylyl CYclase family member (acy-3) [Caenorhabditis elegans] 0.0
blast CAE64497 Hypothetical protein CBG09223 [Caenorhabditis briggsae] 0.0
blast T19934 hypothetical protein C44F1.4 - Caenorhabditis elegans 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 5.8E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497765.2 5 7-29,349-371,376-398,413-435,465-487
C44F1.5 5 7-29,349-371,376-398,413-435,465-487
SNAP00000024034 3 5-27,42-64,94-116
GENEFINDER00000024037 3 13-35,50-72,104-126
Sequence
Target Sequence 
>C. ELEGANS:193920_AT
gggttgaactacgttttctcacattttgaccaagctatagcgaattataaaggagttgag
aaggtgaaaagtgccaatcgattctatattgttgctgttggtctgttgccggatgctgct
caaaatgttaatgaaacgccatggactattggagagcttatgagcacgttggctcatttt
attattagcatttcacaatttgcaatggaaaatgagtttcatgttcagatcggtgttgat
tgcggaagcactcttgcgtttgtagcgaacaccgatcagccccgttatgaaatattcggt
gagactcttgatagatcccgtacactcatgcaagcagccggacatgacacaactctagtt
tctgaagaagtgttcttggctcttcgtcctcgtccgttaagattcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTTGAACTACGTTTTCTCACATT 143 497 1825 Antisense
CTATATTGTTGCTGTTGGTCTGTTG 668 221 1908 Antisense
GAGTTTCATGTTCAGATCGGTGTTG 681 397 2038 Antisense
GCGGAAGCACTCTTGCGTTTGTAGC 248 293 2066 Antisense
GCGTTTGTAGCGAACACCGATCAGC 660 291 2080 Antisense
ACCGATCAGCCCCGTTATGAAATAT 644 87 2095 Antisense
GAGACTCTTGATAGATCCCGTACAC 282 393 2125 Antisense
CCCGTACACTCATGCAAGCAGCCGG 595 259 2141 Antisense
CAGCCGGACATGACACAACTCTAGT 680 201 2159 Antisense
AAGAAGTGTTCTTGGCTCTTCGTCC 468 151 2189 Antisense
TTCGTCCTCGTCCGTTAAGATTCAG 255 695 2207 Antisense
Sequence Source Affymetrix Proprietary Database