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[193919_at] alcohol dehydrogenase; non-specific lipid transfer protein

Description M03A8.1 /REP_DB=WormBase Gene ID /WP=CE04770 /TR=Q21481 /GB=AAA83184.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alcohol dehydrogenase; non-specific lipid transfer protein
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_highest_nonorm > 38      6grp_total > 46      4grp_total > 24      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6511
1 2 3 4 range
A 4894.3
(-3)
P 		6677.4
(-0.8)
P 		9752.6
(3)
P 		9743.9
(3)
P 		4858
B 4960.6
(-3)
P 		6102.9
(-1.9)
P 		9576.4
(1.3)
P 		11405.1
(3)
P 		6445
C 5007.3
(-3)
P 		5621.2
(-2.4)
P 		8841.7
(0.7)
P 		11155
(3)
P 		6148
avr 4954.1
(-3) 		6133.8
(-1.8) 		9390.2
(1.6) 		10768
(3) 		5814

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4311
1 2 3 4 5 6 range
A 9300.3
(3)
P 		6619.6
(-0.7)
P 		7212.4
(0.1)
P 		6435.2
(-1)
P 		4989.6
(-3)
P 		7389.1
(0.3)
P 		4311
B 8568.5
(3)
P 		6210.6
(-1.5)
P 		7213.8
(0.4)
P 		5428.1
(-3)
P 		5602.2
(-2.7)
P 		7253.3
(0.5)
P 		3140
C 7949
(1.8)
P 		6342.3
(-1)
P 		7619.7
(1.2)
P 		6518.8
(-0.7)
P 		5185.8
(-3)
P 		8631.2
(3)
P 		3445
avr 8605.9
(3) 		6390.8
(-1) 		7348.6
(0.7) 		6127.4
(-1.4) 		5259.2
(-3) 		7757.9
(1.5) 		3347
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1655
1 2 3 4 range
A 1782
(-1.1)
P 		1449.5
(-2.9)
P 		2549.5
(3)
P 		1426.3
(-3)
P 		1123
B 1581.9
(-2.5)
P 		1507.3
(-3)
P 		2219.1
(1.6)
P 		2430.4
(3)
P 		923
C 1657.6
(-2.8)
P 		1616.1
(-3)
P 		3081.6
(3)
P 		1880.9
(-1.9)
P 		1466
avr 1673.8
(-2.2) 		1524.3
(-3) 		2616.7
(3) 		1912.5
(-0.9) 		1092

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1671
1 2 3 4 5 6 range
A 1714.4
(-1.5)
P 		2354.3
(3)
P 		1570.7
(-2.5)
P 		1632.1
(-2.1)
P 		1505.6
(-3)
P 		1799
(-0.9)
P 		849
B 2981.4
(3)
P 		2067.2
(-0.6)
P 		1751.7
(-1.8)
P 		1437.7
(-3)
P 		2276.1
(0.3)
P 		2704.7
(1.9)
P 		1544
C 2946.3
(3)
P 		1935.2
(-0.7)
P 		1939.3
(-0.7)
P 		1310.9
(-3)
P 		1678
(-1.7)
P 		2889.4
(2.8)
P 		1635
avr 2547.4
(3) 		2118.9
(0.6) 		1753.9
(-1.4) 		1460.2
(-3) 		1819.9
(-1) 		2464.4
(2.5) 		1087
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193919_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16366
Sequence Type Exemplar sequence
Representative Public ID M03A8.1 NCBI
Target Description M03A8.1 /REP_DB=WormBase Gene ID /WP=CE04770 /TR=Q21481 /GB=AAA83184.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alcohol dehydrogenase; non-specific lipid transfer protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076745(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076745 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-28) (dhs-28) mRNA, complete cds. 11/11 None
GENEFINDER00000018347 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6818699:6819942:1 11/11 None
SNAP00000018337 ENSEMBL cdna:SNAP chromosome:CEL140:X:6818721:6819942:1 11/11 None
M03A8.1 ENSEMBL cdna:known chromosome:CEL140:X:6818391:6820015:1 gene:M03A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6818397-6819942(+) 97.94 100.0
Public Domain and Genome References
Gene Title alcohol dehydrogenase; non-specific lipid transfer protein
Gene Symbol dhs-28
Ensembl M03A8.1
Entrez Gene 180950 Entrez gene
SwissProt Q21481 EMBL-EBI
WormBase CE04770 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604291_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE:1597302_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFA.14480.1.S1_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFA.18106.1.S1_S_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFAAFFX.1219.1.S1_S_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
DROSOPHILA_2:1638695_AT dm
DROSGENOME1:152603_AT dm
CHICKEN:GGA.3546.1.S1_AT hydroxysteroid (17-beta) dehydrogenase 4 gga
HG-U133A:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133_PLUS_2:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133A_2:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-FOCUS:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HUGENEFL:X87176_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:G4504504_3P_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_S_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U95AV2:36626_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_X_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133_PLUS_2:1563696_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
MOUSE430A_2:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOE430A:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430_2:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MU11KSUBB:X89998_S_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MG-U74AV2:97515_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOE430A:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430A_2:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430_2:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MU19KSUBB:TC31197_AT Hydroxysteroid (17-beta) dehydrogenase 4, mRNA (cDNA clone MGC:13992 IMAGE:4209394) mm
MU19KSUBB:TC33135_I_AT Hydroxysteroid (17-beta) dehydrogenase 4, mRNA (cDNA clone MGC:13992 IMAGE:4209394) mm
RT-U34:RC_AI013834_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:RC_AI013834_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:S83279_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:S83279_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:RC_AI101743_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:RC_AI101743_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:S83279_G_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:S83279_G_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RAE230A:1367672_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RAT230_2:1367672_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
YEAST_2:1769308_AT Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5498 sterol carrier activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83184 Dehydrogenases, short chain protein 28 [Caenorhabditis elegans] ref|NP_509146.1| DeHydrogenases, Short chain family member (dhs-28) [Caenorhabditis elegans] 0.0
blast CAE65794 Hypothetical protein CBG10899 [Caenorhabditis briggsae] 0.0
blast CAE65794 Hypothetical protein CBG10899 [Caenorhabditis briggsae] 1.0E-166
blast XP_635235 hypothetical protein DDB0201628 [Dictyostelium discoideum] dbj|BAA94961.1| peroxisomal multifunctional enzyme MFE homolog [Dictyostelium discoideum] gb|EAL61607.1| hypothetical protein DDB0201628 [Dictyostelium discoideum] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.3E-54
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.1E-32
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 4.8E-31
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Sequence
Target Sequence 
>C. ELEGANS:193919_AT
aagctggagctggatggtacggaactattcaatattacaaatctaaaggaaaggttattt
cacacgcatcagctgatgacattgccaagaactggtcaaccatcaccaacatgaacggag
ccgagtacatcggaaccatcactgagcagagtgctagacttgtctcaatcctggaagagc
acgaagcttcatctggaagtagcagtgcttcttctggtgcttcttctggtggtgctttcc
cctcaaacatcaggagtagtgcactgttccaggaaatggctgatggtgtgaaggctgacc
caactgcagtaaaaactttgaaatcgattgtcctttacatcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCTGGAGCTGGATGGTACGGAAC 155 151 719 Antisense
AAAGGTTATTTCACACGCATCAGCT 310 121 768 Antisense
GCCAAGAACTGGTCAACCATCACCA 694 277 802 Antisense
CCATCACCAACATGAACGGAGCCGA 85 271 818 Antisense
TACATCGGAACCATCACTGAGCAGA 608 641 844 Antisense
GAGCAGAGTGCTAGACTTGTCTCAA 612 389 862 Antisense
AGTAGCAGTGCTTCTTCTGGTGCTT 92 63 916 Antisense
TGCTTTCCCCTCAAACATCAGGAGT 550 579 951 Antisense
TCAGGAGTAGTGCACTGTTCCAGGA 673 603 968 Antisense
TGGTGTGAAGGCTGACCCAACTGCA 446 553 1002 Antisense
TGAAATCGATTGTCCTTTACATCAT 211 573 1037 Antisense
Sequence Source Affymetrix Proprietary Database