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[193908_s_at]

Description ZC504.4A /REP_DB=WormBase Gene ID /WP=CE25672 /GEN=mig-15 /TR=Q9XYC3 /GB=CAB63416.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (PAK-STE20 subfamily)
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 40      6grp_total > 15      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 952.8
(3)
P 		769.1
(0.6)
P 		622.6
(-1.3)
P 		486.8
(-3)
P 		466
B 979.6
(3)
P 		715.9
(-0.3)
P 		665.5
(-0.9)
P 		502.3
(-3)
P 		477
C 924.7
(3)
P 		833
(1.6)
P 		723
(-0)
P 		524.4
(-3)
P 		400
avr 952.4
(3) 		772.7
(0.6) 		670.4
(-0.8) 		504.5
(-3) 		448

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 975.1
(3)
P 		855.5
(1.5)
P 		504
(-2.8)
P 		631.8
(-1.2)
P 		561.9
(-2.1)
P 		486.5
(-3)
P 		489
B 1032.1
(3)
P 		852.9
(1.1)
P 		581.5
(-1.8)
P 		603.2
(-1.6)
P 		481.8
(-2.9)
P 		469.4
(-3)
P 		563
C 819.1
(1.5)
P 		925.2
(3)
P 		626
(-1.1)
P 		554.2
(-2.1)
P 		535.6
(-2.3)
P 		487.9
(-3)
P 		437
avr 942.1
(3) 		877.9
(2.2) 		570.5
(-1.8) 		596.4
(-1.5) 		526.4
(-2.4) 		481.3
(-3) 		461
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 346.9
(3)
P 		167
(-0.9)
P 		162.8
(-1)
P 		71.3
(-3)
P 		276
B 312.4
(3)
P 		176.8
(-1)
P 		154.2
(-1.6)
P 		107
(-3)
P 		205
C 306.1
(3)
P 		239.5
(1.2)
P 		252
(1.5)
P 		88.4
(-3)
P 		218
avr 321.8
(3) 		194.4
(-0.3) 		189.7
(-0.4) 		88.9
(-3) 		233

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 179.7
(-0.8)
P 		304.3
(3)
P 		109.8
(-3)
P 		160.2
(-1.4)
P 		169.6
(-1.2)
P 		118.5
(-2.7)
P 		195
B 359.1
(3)
P 		283.9
(0.9)
P 		141.2
(-3)
P 		159.8
(-2.5)
P 		195.7
(-1.5)
P 		175.1
(-2.1)
P 		218
C 303.6
(3)
P 		282.3
(2.3)
P 		159.3
(-1.5)
P 		111.5
(-3)
P 		173.3
(-1.1)
P 		163.3
(-1.4)
P 		192
avr 280.8
(2.6) 		290.2
(3) 		136.8
(-3) 		143.8
(-2.7) 		179.5
(-1.3) 		152.3
(-2.4) 		153
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193908_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17288
Sequence Type Exemplar sequence
Representative Public ID ZC504.4A NCBI
Target Description ZC504.4A /REP_DB=WormBase Gene ID /WP=CE25672 /GEN=mig-15 /TR=Q9XYC3 /GB=CAB63416.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine kinase (PAK-STE20 subfamily)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029800(11), NM_001029802(11), NM_001029801(11), NM_001029803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029800 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-15) (mig-15) mRNA, complete cds. 11/11 None
NM_001029802 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-15) (mig-15) mRNA, complete cds. 11/11 None
NM_001029801 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-15) (mig-15) mRNA, complete cds. 11/11 None
NM_001029803 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-15) (mig-15) mRNA, complete cds. 11/11 None
GENEFINDER00000031668 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10421709:10428079:-1 11/11 None
SNAP00000031666 ENSEMBL cdna:SNAP chromosome:CEL140:X:10422127:10428502:-1 11/11 None
ZC504.4a ENSEMBL cdna:known chromosome:CEL140:X:10421583:10428578:-1 gene:ZC504.4 11/11 A 
ZC504.4c ENSEMBL cdna:known chromosome:CEL140:X:10421583:10428502:-1 gene:ZC504.4 11/11 A 
ZC504.4d ENSEMBL cdna:known chromosome:CEL140:X:10422127:10428502:-1 gene:ZC504.4 11/11 None
ZC504.4b ENSEMBL cdna:known chromosome:CEL140:X:10422127:10428502:-1 gene:ZC504.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10422126-10428502(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC504.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63416 Hypothetical protein ZC504.4a [Caenorhabditis elegans] ref|NP_001024971.1| abnormal cell MIGration family member (mig-15) [Caenorhabditis elegans] gb|AAD14593.1| alternatively spliced serine/threonine protein kinase MIG-15 [Caenorhabditis elegans] 0.0
blast CAC42384 Hypothetical protein ZC504.4c [Caenorhabditis elegans] ref|NP_001024973.1| abnormal cell MIGration family member (mig-15) [Caenorhabditis elegans] sp|Q23356|MIG15_CAEEL Serine/threonine-protein kinase mig-15 (Abnormal cell migration protein 15) 0.0
blast CAB63417 Hypothetical protein ZC504.4b [Caenorhabditis elegans] ref|NP_001024972.1| abnormal cell MIGration family member (mig-15) [Caenorhabditis elegans] 0.0
blast CAD57714 Hypothetical protein ZC504.4d [Caenorhabditis elegans] ref|NP_001024974.1| abnormal cell MIGration family member (mig-15) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.19 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; PhKgM 4.0E-64
hanks 2.1.19 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; PhKgM 3.0E-64
hanks 2.1.19 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; PhKgM 2.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-79
Pfam IPR001180 EMBL-EBI Citron-like 3.6E-103
Pfam IPR001180 EMBL-EBI Citron-like 3.6E-103
Pfam IPR001180 EMBL-EBI Citron-like 3.6E-103
Pfam IPR001180 EMBL-EBI Citron-like 3.6E-103
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-79
Pfam IPR001180 EMBL-EBI Citron-like 3.6E-103
Sequence
Target Sequence 
>C. ELEGANS:193908_S_AT
ggcaccgaagccataccataagttcatgagtttcaagtcattcggatctttgagtcatgt
tcctttgattgtcgacttgacagttgaagacaatgcaagactcaaggttctttatggatc
gacaggtggattccatgcaattgatttggattctgcagcggtctatgatatttatacgcc
agcacagagcggtcaaactacgactccacattgcatagttgttcttccaaactccaatgg
aatgcaactccttctctgttatgacaatgagggagtatatgttaacacgtacggaaggat
gacaaagaatgtagtcctgcaatggggagaaatgccaagcagtgtggcgtacatttcaac
gggtcagattatgggatggggcaacaaggcgattgagattcgatccgttgacactggcca
tttggatggagtgtttatgcacaagaaagctcagaagctgaaattcttgtgtgagagaaa
tgataaagtattcttctcgtctgcgaagggtggagggtcttgccagatctacttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACCGAAGCCATACCATAAGTTC 424 533 2706 Antisense
ATGTTCCTTTGATTGTCGACTTGAC 676 45 2762 Antisense
GATTTGGATTCTGCAGCGGTCTATG 616 427 2848 Antisense
ATACGCCAGCACAGAGCGGTCAAAC 321 21 2879 Antisense
GTCAAACTACGACTCCACATTGCAT 215 463 2897 Antisense
GCATAGTTGTTCTTCCAAACTCCAA 486 307 2918 Antisense
CAATGGAATGCAACTCCTTCTCTGT 353 183 2940 Antisense
GTGTGGCGTACATTTCAACGGGTCA 479 485 3047 Antisense
TGAGATTCGATCCGTTGACACTGGC 599 567 3099 Antisense
GTATTCTTCTCGTCTGCGAAGGGTG 328 453 3193 Antisense
TGGAGGGTCTTGCCAGATCTACTTC 101 549 3216 Antisense
Sequence Source Affymetrix Proprietary Database