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[193897_at] Phorbol esters / diacylglycerol binding domain (2 domains)

Description R12G8.1 /REP_DB=WormBase Gene ID /WP=CE16313 /TR=O45720 /GB=CAB07853.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phorbol esters - diacylglycerol binding domain (2 domains)
Cluster 6grp_total > 15      4grp_total > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 398
1 2 3 4 range
A 16.7
(-2.9)
A 		17.8
(-2.9)
A 		13.8
(-3)
A 		305.7
(3)
A 		292
B 18.4
(-2.9)
A 		9.4
(-3)
A 		18.2
(-2.9)
A 		407
(3)
P 		398
C 22.5
(-3)
A 		22.8
(-3)
A 		29
(-2.8)
A 		270.7
(3)
A 		248
avr 19.2
(-3) 		16.7
(-3) 		20.3
(-2.9) 		327.8
(3) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 33.7
(-1.8)
A 		102.9
(3)
A 		59.2
(-0)
A 		16.9
(-3)
A 		23.7
(-2.5)
A 		21.2
(-2.7)
A 		86
B 102.2
(3)
A 		71.2
(0.9)
A 		14.9
(-3)
A 		16.3
(-2.9)
A 		22.8
(-2.5)
A 		16.4
(-2.9)
A 		87
C 129
(2.4)
A 		141
(3)
A 		20.5
(-2.8)
A 		17.1
(-3)
A 		40.6
(-1.9)
A 		18.9
(-2.9)
A 		124
avr 88.3
(1.9) 		105
(3) 		31.5
(-2) 		16.8
(-3) 		29
(-2.2) 		18.8
(-2.9) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 6.1
(-2.6)
A 		3.9
(-3)
A 		3.6
(-3)
A 		44.7
(3)
A 		41
B 5.9
(-2.7)
A 		2.3
(-3)
A 		4.2
(-2.9)
A 		86.7
(3)
P 		84
C 7.4
(-2.9)
A 		6.6
(-3)
A 		10.1
(-2.5)
A 		45.7
(3)
A 		39
avr 6.5
(-2.8) 		4.3
(-3) 		6
(-2.8) 		59
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 6.2
(-2.6)
A 		36.6
(3)
A 		12.9
(-1.4)
A 		4.3
(-3)
A 		7.2
(-2.5)
A 		5.2
(-2.8)
A 		32
B 35.6
(3)
A 		23.7
(0.8)
A 		3.6
(-3)
A 		4.3
(-2.9)
A 		9.2
(-2)
A 		6.1
(-2.5)
A 		32
C 47.8
(3)
A 		43
(2.4)
A 		5.2
(-2.8)
A 		3.4
(-3)
A 		13.1
(-1.7)
A 		6.3
(-2.6)
A 		44
avr 29.9
(2.1) 		34.4
(3) 		7.2
(-2.4) 		4
(-3) 		9.8
(-1.9) 		5.9
(-2.6) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193897_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14387
Sequence Type Exemplar sequence
Representative Public ID R12G8.1 NCBI
Target Description R12G8.1 /REP_DB=WormBase Gene ID /WP=CE16313 /TR=O45720 /GB=CAB07853.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Phorbol esters - diacylglycerol binding domain (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075085(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075085 NCBI Caenorhabditis elegans R12G8.1 (R12G8.1) mRNA, complete cds. 11/11 None
SNAP00000026660 ENSEMBL cdna:SNAP chromosome:CEL140:V:17750657:17751799:-1 11/11 None
GENEFINDER00000026662 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17749843:17751799:-1 11/11 None
R12G8.1 ENSEMBL cdna:known chromosome:CEL140:V:17750657:17751799:-1 gene:R12G8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17750656-17751799(-) 100.0 100.0
Public Domain and Genome References
Gene Title Phorbol esters / diacylglycerol binding domain (2 domains)
Ensembl R12G8.1
Entrez Gene 187844 Entrez gene
SwissProt O45720 EMBL-EBI
WormBase CE16313 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07853 Hypothetical protein R12G8.1 [Caenorhabditis elegans] ref|NP_507486.1| R12G8.1 [Caenorhabditis elegans] 1.0E-89
blast CAB07853 Hypothetical protein R12G8.1 [Caenorhabditis elegans] ref|NP_507486.1| R12G8.1 [Caenorhabditis elegans] 7.0E-84
blast CAE61713 Hypothetical protein CBG05662 [Caenorhabditis briggsae] 3.0E-67
blast CAE61713 Hypothetical protein CBG05662 [Caenorhabditis briggsae] 3.0E-63
blast AAG24119 Hypothetical protein F48G7.10 [Caenorhabditis elegans] ref|NP_503273.1| F48G7.10 [Caenorhabditis elegans] 3.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.9E-17
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 8.2E-21
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.4E-18
Sequence
Target Sequence 
>C. ELEGANS:193897_AT
ggcaccggaactgcaaattcctaccgtaacccgtcaatcggatggtactaagatccatga
aatttcgggccacaagttcaaagccgtcggacttgctcagccgacgttttgctcattttg
ctccaaattcatatatggtgttggaaaacagggttacaagtgtctaggctgtgagacagt
cgttcataagcggtgccatgccctcatcaccgcccgctgcaccttcggaccatcttcccg
agctccggttcctgctgaaactacaaatctgaaaactcgtcgtagccgatctgcttcgga
agaacctccatcaaatactcatcactttaacaagcatttcttcactcgcccgacattttg
tgatcactgtggaagtatgctctatgggctcacgaagcaaggcgttcagtgcacggattg
tcatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACCGGAACTGCAAATTCCTACC 607 533 21 Antisense
TTCAAAGCCGTCGGACTTGCTCAGC 70 687 97 Antisense
GACAGTCGTTCATAAGCGGTGCCAT 429 365 195 Antisense
CTCCGGTTCCTGCTGAAACTACAAA 514 233 263 Antisense
TCGTCGTAGCCGATCTGCTTCGGAA 427 597 297 Antisense
GGAAGAACCTCCATCAAATACTCAT 80 527 318 Antisense
TTTAACAAGCATTTCTTCACTCGCC 489 673 346 Antisense
TTCACTCGCCCGACATTTTGTGATC 398 687 361 Antisense
GGAAGTATGCTCTATGGGCTCACGA 282 531 391 Antisense
GGGCTCACGAAGCAAGGCGTTCAGT 433 491 406 Antisense
GGCGTTCAGTGCACGGATTGTCATG 618 541 421 Antisense
Sequence Source Affymetrix Proprietary Database