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[193889_at] mitotic chromosome and X-chromosome associated MIX-1 protein

Description M106.1 /REP_DB=WormBase Gene ID /WP=CE18083 /GEN=mix-1 /GB=CAA20330.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=mitotic chromosome and X-chromosome associated MIX-1 protein
Cluster 6grp_total > 37      4grp_total > 55      6grp_cgc > 15      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 373
1 2 3 4 range
A 616.5
(3)
P 		416.9
(-1.1)
A 		475.6
(0.1)
M 		323.1
(-3)
A 		293
B 696.4
(3)
P 		456.4
(-2)
P 		488.8
(-1.3)
M 		406.8
(-3)
A 		290
C 595.8
(3)
P 		360.9
(-2.5)
A 		490.5
(0.6)
P 		337.4
(-3)
M 		258
avr 636.2
(3) 		411.4
(-1.8) 		485
(-0.2) 		355.8
(-3) 		280

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 384
1 2 3 4 5 6 range
A 572.8
(2.5)
P 		577.6
(2.6)
P 		378
(-3)
P 		536.1
(1.5)
A 		399.2
(-2.4)
A 		590.9
(3)
P 		213
B 514.3
(2)
P 		538.9
(3)
P 		428
(-1.5)
P 		505
(1.6)
M 		391.6
(-3)
M 		469.8
(0.2)
P 		147
C 762.4
(3)
P 		583.5
(-0.1)
P 		502.6
(-1.4)
P 		448.7
(-2.4)
P 		411.5
(-3)
A 		485.6
(-1.7)
P 		351
avr 616.5
(3) 		566.7
(1.6) 		436.2
(-2) 		496.6
(-0.3) 		400.8
(-3) 		515.4
(0.2) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 224.5
(3)
P 		90.5
(-1.5)
A 		124.3
(-0.4)
M 		47.3
(-3)
A 		177
B 222.1
(3)
P 		112.7
(-1.8)
P 		113.3
(-1.8)
M 		86.7
(-3)
A 		135
C 197.2
(3)
P 		103.8
(-1)
A 		171
(1.9)
P 		56.9
(-3)
M 		140
avr 214.6
(3) 		102.3
(-1.5) 		136.2
(-0.1) 		63.6
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 105.6
(-1.9)
P 		205.4
(3)
P 		82.3
(-3)
P 		136
(-0.4)
A 		120.5
(-1.1)
A 		143.9
(0)
P 		123
B 179
(3)
P 		179.4
(3)
P 		103.9
(-3)
P 		133.8
(-0.6)
M 		159.1
(1.4)
M 		175.2
(2.7)
P 		76
C 282.6
(3)
P 		178
(-0.3)
P 		127.9
(-1.8)
P 		90.2
(-3)
P 		133.1
(-1.7)
A 		162.5
(-0.7)
P 		192
avr 189.1
(3) 		187.6
(2.9) 		104.7
(-3) 		120
(-1.9) 		137.6
(-0.7) 		160.5
(1) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193889_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3318
Sequence Type Exemplar sequence
Representative Public ID M106.1 NCBI
Target Description M106.1 /REP_DB=WormBase Gene ID /WP=CE18083 /GEN=mix-1 /GB=CAA20330.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=mitotic chromosome and X-chromosome associated MIX-1 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063930(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063930 NCBI Caenorhabditis elegans MItosis and X associated family member (mix-1) (mix-1) mRNA, complete cds. 11/11 A 
SNAP00000018716 ENSEMBL cdna:SNAP chromosome:CEL140:II:10836243:10841131:1 11/11 None
GENEFINDER00000018728 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10835695:10841131:1 11/11 None
M106.1 ENSEMBL cdna:known chromosome:CEL140:II:10824206:10841319:1 gene:M106.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10824291-10841128(+) 100.0 100.0
Public Domain and Genome References
Gene Title mitotic chromosome and X-chromosome associated MIX-1 protein
Gene Symbol mix-1
Ensembl M106.1
Entrez Gene 174669 Entrez gene
SwissProt Q09591 EMBL-EBI
WormBase CE18083 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
7059 chromosome segregation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
793 condensed chromosome inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87054 Hypothetical protein M106.1 [Caenorhabditis elegans] emb|CAA86786.1| Hypothetical protein M106.1 [Caenorhabditis elegans] emb|CAA20330.1| Hypothetical protein M106.1 [Caenorhabditis elegans] ref|NP_496331.1| MItosis and X associated family member (mix-1) [Caenorhabditis elegans] sp|Q09591|MIX1_CAEEL Mitotic chromosome and X-chromosome associated protein mix-1 (Structural maintenance of chromosome 2) (Protein let-29) gb|AAC47834.1| mitotic chromosome and X-chromosome associated MIX-1 protein [Caenorhabditis elegans] 0.0
blast CAE59599 Hypothetical protein CBG03006 [Caenorhabditis briggsae] 0.0
blast T23981 hypothetical protein R06F6.10 - Caenorhabditis elegans 0.0
blast CAE59599 Hypothetical protein CBG03006 [Caenorhabditis briggsae] 1.0E-173
Protein Domains
Database ID Description E-Value
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 5.59999990463257
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 4.09999990463257
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 2.0
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 4.09999990463257
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 2.70000004768372
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 1.0E-19
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 2.4E-20
Pfam IPR003405 EMBL-EBI Structural maintenance of chromosome protein SMC, C-terminal 1.0E-25
Pfam IPR003395 EMBL-EBI SMC protein, N-terminal 4.6E-47
Pfam IPR010935 EMBL-EBI SMCs flexible hinge 9.5E-38
Sequence
Target Sequence 
>C. ELEGANS:193889_AT
ttggacaaatcttcaactgcctgcttcccgatgcccatgcctcactggtacctccagagg
gaaagacagtttgtgagggtcttgaagtgaaagtctcattcggtggagtcgtcaaggata
gtttacatgaactttctggaggacagcggtctcttgtcgcgttgtcactgattttagcta
tgctgaaattcaagccagctccattgtatattctcgacgaagttgatgcggcccttgatt
tgtcacacaccgcgaatattggaatgatgatcaagacgcatttccatcataatcagttca
tcatcgtctcactcaaacaaggaatgttctcaaatgcagatgttctcttccaaacacgtt
ttgccgatggacattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGACAAATCTTCAACTGCCTGCT 329 709 3179 Antisense
GCCTCACTGGTACCTCCAGAGGGAA 242 283 3217 Antisense
GAAAGTCTCATTCGGTGGAGTCGTC 598 359 3267 Antisense
CTTTCTGGAGGACAGCGGTCTCTTG 423 221 3310 Antisense
TCTTGTCGCGTTGTCACTGATTTTA 534 617 3330 Antisense
GCCAGCTCCATTGTATATTCTCGAC 455 279 3372 Antisense
CGAAGTTGATGCGGCCCTTGATTTG 147 253 3396 Antisense
CTTGATTTGTCACACACCGCGAATA 201 215 3412 Antisense
GACGCATTTCCATCATAATCAGTTC 13 381 3453 Antisense
AGATGTTCTCTTCCAAACACGTTTT 25 69 3516 Antisense
AACACGTTTTGCCGATGGACATTCT 625 135 3531 Antisense
Sequence Source Affymetrix Proprietary Database