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[193887_s_at] NADH-ubiquinone oxidoreductase 49KD subunit

Description K09A9.5 /REP_DB=WormBase Gene ID /WP=CE11980 /GEN=gas-1 /TR=SW:Q93873 /GB=CAB01886.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 49KD subunit
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 41      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 25      6grp_total > 42      4grp_total > 51      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3557
1 2 3 4 range
A 4317.7
(-0)
P 		6096
(3)
P 		5029.4
(1.2)
P 		2548.5
(-3)
P 		3548
B 4619.9
(0.1)
P 		5334.4
(1.5)
P 		6076.2
(3)
P 		3055.5
(-3)
P 		3021
C 4413.3
(0.4)
P 		5856.6
(3)
P 		4570.6
(0.7)
P 		2538.8
(-3)
P 		3318
avr 4450.3
(0.4) 		5762.3
(3) 		5225.4
(1.9) 		2714.3
(-3) 		3048

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3220
1 2 3 4 5 6 range
A 3577.5
(-0.2)
P 		4783.1
(3)
P 		4541.1
(2.4)
P 		4089.9
(1.1)
P 		2548
(-3)
P 		2797.4
(-2.3)
P 		2235
B 3469.1
(-0.8)
P 		3974.3
(0.2)
P 		5494.9
(3)
P 		3849.9
(-0.1)
P 		2981.1
(-1.7)
P 		2275.4
(-3)
P 		3220
C 4709
(2)
P 		3748.6
(-0.1)
P 		5177.6
(3)
P 		3843.7
(0.1)
P 		3847.7
(0.1)
P 		2419.4
(-3)
P 		2758
avr 3918.5
(0.3) 		4168.7
(0.9) 		5071.2
(3) 		3927.8
(0.3) 		3125.6
(-1.5) 		2497.4
(-3) 		2574
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1311
1 2 3 4 range
A 1572.1
(3)
P 		1323.3
(1.8)
P 		1314.8
(1.7)
P 		373
(-3)
P 		1199
B 1473.2
(3)
P 		1317.5
(1.9)
P 		1408
(2.5)
P 		651.1
(-3)
P 		822
C 1460.9
(1.9)
P 		1683.8
(3)
P 		1593
(2.6)
P 		428.1
(-3)
P 		1256
avr 1502.1
(3) 		1441.5
(2.6) 		1438.6
(2.6) 		484.1
(-3) 		1018

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1086
1 2 3 4 5 6 range
A 659.5
(-3)
P 		1701.1
(3)
P 		989
(-1.1)
P 		1037.3
(-0.8)
P 		768.9
(-2.4)
P 		681.1
(-2.9)
P 		1042
B 1207.1
(1.4)
P 		1322.9
(2.9)
P 		1334.3
(3)
P 		1019.7
(-0.9)
P 		1211.2
(1.5)
P 		848.5
(-3)
P 		486
C 1745.4
(3)
P 		1143.8
(-0.7)
P 		1317.8
(0.4)
P 		773
(-3)
P 		1245.1
(-0.1)
P 		809.9
(-2.8)
P 		972
avr 1204
(1.2) 		1389.3
(3) 		1213.7
(1.3) 		943.3
(-1.4) 		1075.1
(-0.1) 		779.8
(-3) 		610
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193887_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17917
Sequence Type Exemplar sequence
Representative Public ID K09A9.5 NCBI
Target Description K09A9.5 /REP_DB=WormBase Gene ID /WP=CE11980 /GEN=gas-1 /TR=SW:Q93873 /GB=CAB01886.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase 49KD subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078168(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078168 NCBI Caenorhabditis elegans General Anaesthetic Sensitivity abnormal family member (gas-1) (gas-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000037626 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:15586937:15590309:-1 11/11 None
SNAP00000037620 ENSEMBL cdna:SNAP chromosome:CEL140:X:15588615:15590309:-1 11/11 None
K09A9.5.1 ENSEMBL cdna:known chromosome:CEL140:X:15586731:15590315:-1 gene:K09A9.5 11/11 None
K09A9.5.2 ENSEMBL cdna:known chromosome:CEL140:X:15586731:15590317:-1 gene:K09A9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15586936-15590309(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase 49KD subunit
Gene Symbol gas-1
Ensembl K09A9.5
Entrez Gene 181646 Entrez gene
SwissProt Q93873 EMBL-EBI
WormBase CE11980 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1630695_AT dm
DROSGENOME1:150980_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01886 Hypothetical protein K09A9.5 [Caenorhabditis elegans] ref|NP_510569.1| General Anaesthetic Sensitivity abnormal family member (gas-1) [Caenorhabditis elegans] sp|Q93873|NUCM_CAEEL Probable NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor (Complex I-49KD) (CI-49KD) 0.0
blast CAE63330 Hypothetical protein CBG07730 [Caenorhabditis briggsae] 0.0
blast NP_498423 Temporarily Assigned Gene name family member (tag-99) [Caenorhabditis elegans] gb|AAC77506.1| Temporarily assigned gene name protein 99 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001135 EMBL-EBI NADH-ubiquinone oxidoreductase, chain 49kDa 1.0E-126
Pfam IPR001135 EMBL-EBI NADH-ubiquinone oxidoreductase, chain 49kDa 1.0E-126
Pfam IPR001135 EMBL-EBI NADH-ubiquinone oxidoreductase, chain 49kDa 1.2E-20
Pfam IPR001135 EMBL-EBI NADH-ubiquinone oxidoreductase, chain 49kDa 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193887_S_AT
gacatgctcaccgaaaaccgaatctggaaggccagaactattgacatcggacttgtgtca
gctgctgatgctctcaactggggattcagtggagtcatggtccgtggatctggaatcaag
caagacgtgagaaagaccgaaccatacgatgcctatgctgatatggaatttgatgttcca
atcggaaccaagggtgattgctacgacagatacttgtgccgtattgaggagatgagacag
tcgttgaacattgtgcatcagtgcctcaacaagatgccagccggagaaatcaaggtcgac
gatcacaaggttgtgccaccaaagagagccgagatgaaggagaacatggagtctcttatt
catcatttcaagttcttcaccgaaggattccaagtgccaccagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACATGCTCACCGAAAACCGAATCT 58 369 847 Antisense
AGGCCAGAACTATTGACATCGGACT 113 49 875 Antisense
ACATCGGACTTGTGTCAGCTGCTGA 594 105 890 Antisense
GCTGATGCTCTCAACTGGGGATTCA 368 295 910 Antisense
AGTGGAGTCATGGTCCGTGGATCTG 76 59 934 Antisense
GACCGAACCATACGATGCCTATGCT 238 383 981 Antisense
GAACATTGTGCATCAGTGCCTCAAC 281 351 1092 Antisense
GCCTCAACAAGATGCCAGCCGGAGA 539 281 1109 Antisense
AGGTTGTGCCACCAAAGAGAGCCGA 498 57 1154 Antisense
TCATCATTTCAAGTTCTTCACCGAA 319 621 1206 Antisense
CCGAAGGATTCCAAGTGCCACCAGG 295 263 1226 Antisense
Sequence Source Affymetrix Proprietary Database