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[193841_s_at] Domain found in Dishevelled, Egl-10, and Pleckstrin

Description F55H2.4 /REP_DB=WormBase Gene ID /WP=CE00211 /TR=SW:P34464 /GB=CAA81602.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Domain found in Dishevelled, Egl-10, and Pleckstrin
Cluster 6grp_total > 98      4grp_total > 27      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 29.1
(-0.2)
A 		15.5
(-3)
A 		24.8
(-1.1)
A 		44.3
(3)
A 		29
B 40.9
(1.1)
A 		50.1
(3)
P 		28.3
(-1.6)
A 		21.5
(-3)
A 		29
C 10.5
(-3)
A 		34.1
(2.5)
A 		14.7
(-2)
A 		36.2
(3)
A 		26
avr 26.8
(-0.8) 		33.2
(2.6) 		22.6
(-3) 		34
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 3.5
(-3)
A 		23.9
(-2.2)
A 		4.4
(-3)
A 		30.6
(-1.9)
A 		121.1
(1.7)
P 		154.7
(3)
P 		151
B 23
(-2.3)
A 		10.8
(-2.8)
A 		4.5
(-3)
A 		34.6
(-1.9)
A 		114.3
(1)
P 		168.4
(3)
P 		164
C 16
(-2.5)
A 		8.7
(-2.8)
A 		4.7
(-3)
A 		48.6
(-1.1)
A 		144.6
(3)
P 		145.3
(3)
P 		141
avr 14.2
(-2.6) 		14.5
(-2.6) 		4.5
(-3) 		37.9
(-1.7) 		126.7
(1.8) 		156.1
(3) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 10.6
(3)
A 		3.4
(-3)
A 		6.5
(-0.4)
A 		6.5
(-0.4)
A 		7
B 13.1
(3)
A 		12.4
(2.5)
P 		6.6
(-1.6)
A 		4.6
(-3)
A 		9
C 3.5
(-3)
A 		9.8
(3)
A 		5.1
(-1.5)
A 		6.1
(-0.5)
A 		6
avr 9.1
(3) 		8.5
(1.9) 		6.1
(-2.3) 		5.7
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 0.6
(-3)
A 		8.5
(-1.7)
A 		1
(-2.9)
A 		7.8
(-1.8)
A 		36.5
(2.8)
P 		37.7
(3)
P 		37
B 8
(-2.3)
A 		3.6
(-2.8)
A 		1.1
(-3)
A 		9.2
(-2.2)
A 		46.4
(1.4)
P 		62.8
(3)
P 		62
C 5.9
(-2.4)
A 		2.7
(-2.8)
A 		1.2
(-3)
A 		9.8
(-1.9)
A 		46.8
(2.8)
P 		48.6
(3)
P 		47
avr 4.8
(-2.5) 		4.9
(-2.5) 		1.1
(-3) 		8.9
(-2) 		43.2
(2.2) 		49.7
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193841_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7390
Sequence Type Exemplar sequence
Representative Public ID F55H2.4 NCBI
Target Description F55H2.4 /REP_DB=WormBase Gene ID /WP=CE00211 /TR=SW:P34464 /GB=CAA81602.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Domain found in Dishevelled, Egl-10, and Pleckstrin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070006(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070006 NCBI Caenorhabditis elegans LEThal family member (let-99) (let-99) mRNA, complete cds. 11/11 A 
SNAP00000007116 ENSEMBL cdna:SNAP chromosome:CEL140:III:9504660:9506977:1 10/11 None
GENEFINDER00000007123 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9504660:9507066:1 9/11 None
SNAP00000042644 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12567827:12570418:1 11/11 None
GENEFINDER00000042660 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12567827:12570418:1 11/11 None
F55H2.4 ENSEMBL cdna:pseudogene chromosome:CEL140:III:9504660:9506977:1 gene:F55H2.4 11/11 A 
K08E7.3 ENSEMBL cdna:known chromosome:CEL140:IV:12567827:12570576:1 gene:K08E7.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9504655-9505898(+) 100.0 100.0
Public Domain and Genome References
Gene Title Domain found in Dishevelled, Egl-10, and Pleckstrin
Gene Symbol let-99
Ensembl F55H2.4
K08E7.3
Entrez Gene 178210 Entrez gene
SwissProt Q21341 EMBL-EBI
WormBase CE06147 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01225 Hypothetical protein K08E7.3 [Caenorhabditis elegans] ref|NP_502407.1| LEThal family member (let-99) [Caenorhabditis elegans] sp|Q21341|LET99_CAEEL Lethal protein 0.0
blast CAE65196 Hypothetical protein CBG10071 [Caenorhabditis briggsae] 0.0
blast P34464 Hypothetical protein F55H2.4 in chromosome III pir||S40987 hypothetical protein F55H2.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 7.7E-18
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 3.9E-15
Sequence
Target Sequence 
>C. ELEGANS:193841_S_AT
cgaaagtcaatcaaccaacccgtcgttcaacatctcttgatcgaaatcatcgtgtactgg
aacaggaaaaagctgaaactgctcttcgtaaccagaaagtaaccactccgccaattcgag
aagctccaaaagatgtggtcttcatgcatccacaaagaccattgcctgcagttcctagca
gatatcaaaatcatcagacaaacatcgcaggatcaaatccagcattgttatcgcgtggaa
gaatgtacgaatccatcatgagaagatcgtcggttgttccagttgcagactctctccagc
cgaacaacgaatgctcattgtggaaaaaagagttgcttggacgattggaacagatttacg
atcgcactcttccctgtgaatgggcgtctaaagtcgacggacacgatattcaatggaaca
tgcttgaaatagatcatgttgatggaatcgtcaaatcaagatgtcagggactgcagccag
attatccggaaactgtcattcaattcatggattatcttgttcgctatccatttgtcacgt
ccaaaaaggcggatacaatgggggaatacaacgtcaatcggattttcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAAGTCAATCAACCAACCCGTCG 589 251 542 Antisense
TTGCCTGCAGTTCCTAGCAGATATC 221 701 703 Antisense
GCAGTTCCTAGCAGATATCAAAATC 8 313 709 Antisense
CATCGCAGGATCAAATCCAGCATTG 551 211 744 Antisense
AATCCAGCATTGTTATCGCGTGGAA 704 165 757 Antisense
GGAAGAATGTACGAATCCATCATGA 583 529 778 Antisense
GAATCCATCATGAGAAGATCGTCGG 139 329 790 Antisense
GAGAAGATCGTCGGTTGTTCCAGTT 480 391 801 Antisense
TCGTCGGTTGTTCCAGTTGCAGACT 436 605 808 Antisense
TTCAATTCATGGATTATCTTGTTCG 496 685 1040 Antisense
ATACAACGTCAATCGGATTTTCATA 584 23 1107 Antisense
Sequence Source Affymetrix Proprietary Database