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[193827_s_at]

Description H35B03.2A /REP_DB=WormBase Gene ID /WP=CE19505 /TR=Q9TYZ2 /GB=AAC68971.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=C45E5.5 was incorporated into this gene - 980715 -js
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 86      4grp_highest_8_8 > 12      4grp_high_8_8 > 30      6grp_highest_nonorm > 82      6grp_total > 87      4grp_total > 33      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 234
1 2 3 4 range
A 249.4
(-3)
P 		353.6
(0.2)
P 		442.8
(3)
P 		377.2
(1)
P 		193
B 311.9
(-3)
P 		407
(1.4)
P 		441.1
(3)
P 		428.3
(2.4)
P 		129
C 355
(-3)
P 		426.4
(0.3)
P 		373
(-2.2)
P 		483.8
(3)
P 		129
avr 305.4
(-3) 		395.7
(1.4) 		419
(2.5) 		429.8
(3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 308.7
(-1.9)
P 		266.1
(-3)
P 		378.6
(0)
P 		346.6
(-0.8)
P 		425.4
(1.3)
P 		488.6
(3)
P 		223
B 264.6
(-3)
P 		362.9
(-0.5)
P 		335.1
(-1.2)
P 		325.7
(-1.4)
P 		442.9
(1.6)
P 		498.8
(3)
P 		234
C 267.6
(-3)
P 		336.8
(-1.6)
P 		311
(-2.1)
P 		313.5
(-2.1)
P 		352.5
(-1.3)
P 		562.1
(3)
P 		295
avr 280.3
(-3) 		321.9
(-1.9) 		341.6
(-1.4) 		328.6
(-1.8) 		406.9
(0.2) 		516.5
(3) 		236
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 90.8
(0.5)
P 		76.8
(-0.9)
P 		115.7
(3)
P 		55.2
(-3)
P 		61
B 99.5
(1.5)
P 		100.5
(2.1)
P 		102.2
(3)
P 		91.3
(-3)
P 		11
C 117.5
(1.5)
P 		122.6
(2.1)
P 		130
(3)
P 		81.6
(-3)
P 		48
avr 102.6
(1) 		100
(0.6) 		116
(3) 		76
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 56.9
(-3)
P 		94.6
(0.2)
P 		82.5
(-0.9)
P 		87.9
(-0.4)
P 		128.4
(3)
P 		119
(2.2)
P 		72
B 92.1
(-2.4)
P 		120.8
(-0.7)
P 		81.4
(-3)
P 		86.3
(-2.7)
P 		180
(2.7)
P 		186
(3)
P 		105
C 99.2
(-1.3)
P 		102.8
(-1.1)
P 		79.2
(-2.2)
P 		63
(-3)
P 		114.1
(-0.6)
P 		188.2
(3)
P 		125
avr 82.7
(-2.7) 		106.1
(-1.1) 		81
(-2.9) 		79.1
(-3) 		140.8
(1.3) 		164.4
(3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193827_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11212
Sequence Type Exemplar sequence
Representative Public ID H35B03.2A NCBI
Target Description H35B03.2A /REP_DB=WormBase Gene ID /WP=CE19505 /TR=Q9TYZ2 /GB=AAC68971.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=C45E5.5 was incorporated into this gene - 980715 -js
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068451(11), NM_068452(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068451 NCBI Caenorhabditis elegans H35B03.2a (H35B03.2) mRNA, complete cds. 11/11 None
NM_068452 NCBI Caenorhabditis elegans H35B03.2b (H35B03.2) mRNA, complete cds. 11/11 None
H35B03.2a ENSEMBL cdna:known chromosome:CEL140:IV:5729646:5732621:-1 gene:H35B03.2 11/11 None
H35B03.2b ENSEMBL cdna:known chromosome:CEL140:IV:5729787:5730754:-1 gene:H35B03.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5729792-5731665(-) 98.73 98.73
Public Domain and Genome References
Ensembl H35B03.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68971 Hypothetical protein H35B03.2a [Caenorhabditis elegans] ref|NP_500852.1| H35B03.2a [Caenorhabditis elegans] 0.0
blast CAE72661 Hypothetical protein CBG19874 [Caenorhabditis briggsae] 1.0E-164
blast AAC68972 Hypothetical protein H35B03.2b [Caenorhabditis elegans] ref|NP_500853.1| H35B03.2b [Caenorhabditis elegans] 2.0E-72
blast AAC68971 Hypothetical protein H35B03.2a [Caenorhabditis elegans] ref|NP_500852.1| H35B03.2a [Caenorhabditis elegans] 2.0E-72
blast AAC68972 Hypothetical protein H35B03.2b [Caenorhabditis elegans] ref|NP_500853.1| H35B03.2b [Caenorhabditis elegans] 8.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR002738 EMBL-EBI RNase P subunit p30 3.9E-65
Pfam IPR002738 EMBL-EBI RNase P subunit p30 1.5E-35
Sequence
Target Sequence 
>C. ELEGANS:193827_S_AT
gcagagagtttgatgcctgccacacggaaaaatacattgttcaacggccgagtcctgatc
agatccctaaaatcgaagaacataatcttctcatccggtgctgaaacaatgcttgatctc
cgttccccagtcgacgtgatgaatatgtctcttttgtggggtgttgcaaataacgaagca
cgcaaaatgatttccggtttcccgaagaaccttctccttcaagcggaatgccgtggaact
ggaaatggagatgtttgttcgatgaagttgagtgatgcagaaaaattggcaggaaatgga
gaagaagaagagcaggatggagcttctgaagagaaggttgttgttggaaaagaagaagaa
gccgagaaagtgaaagctccaccgattgaagttagaatgaatcattctcaatatcaggca
ctgctacaggcaactaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGAGAGTTTGATGCCTGCCACAC 373 313 700 Antisense
CCGAGTCCTGATCAGATCCCTAAAA 95 265 747 Antisense
TAATCTTCTCATCCGGTGCTGAAAC 655 635 782 Antisense
TGAAACAATGCTTGATCTCCGTTCC 420 573 801 Antisense
GTTCCCCAGTCGACGTGATGAATAT 196 441 821 Antisense
ATATGTCTCTTTTGTGGGGTGTTGC 579 21 842 Antisense
GAAGCACGCAAAATGATTTCCGGTT 556 339 874 Antisense
GAACCTTCTCCTTCAAGCGGAATGC 637 341 906 Antisense
GAAAGCTCCACCGATTGAAGTTAGA 102 359 1071 Antisense
ATCATTCTCAATATCAGGCACTGCT 190 31 1100 Antisense
ATCAGGCACTGCTACAGGCAACTAA 315 29 1112 Antisense
Sequence Source Affymetrix Proprietary Database