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[193823_at]

Description R05A10.1 /REP_DB=WormBase Gene ID /WP=CE28066 /TR=O45699 /GB=CAB05263.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Reverse transcriptase (RNA-dependent DNA polymerase)
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 58      4grp_high_8_8 > 04      6grp_highest_nonorm > 92      6grp_total > 59      4grp_total > 05      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 852
1 2 3 4 range
A 384.3
(-0.2)
P 		94
(-2.5)
A 		26.7
(-3)
A 		796.4
(3)
P 		770
B 309
(-0.8)
P 		104
(-2.5)
A 		43.4
(-3)
P 		759.9
(3)
P 		717
C 340.8
(-0.9)
P 		64.4
(-2.9)
P 		47.8
(-3)
A 		878.9
(3)
P 		831
avr 344.7
(-0.6) 		87.5
(-2.6) 		39.3
(-3) 		811.7
(3) 		772

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 608
1 2 3 4 5 6 range
A 368.6
(-0.2)
P 		203.5
(-2.1)
P 		193.8
(-2.2)
P 		121.6
(-3)
P 		302.3
(-0.9)
P 		647.2
(3)
P 		526
B 485.7
(1.2)
P 		176.4
(-2.2)
P 		135.5
(-2.7)
P 		105.5
(-3)
P 		312.1
(-0.7)
P 		643.2
(3)
P 		538
C 697
(3)
P 		231.6
(-1.6)
P 		120.8
(-2.7)
P 		89.3
(-3)
P 		316.8
(-0.8)
P 		643.8
(2.5)
P 		608
avr 517.1
(1.6) 		203.8
(-1.9) 		150
(-2.5) 		105.5
(-3) 		310.4
(-0.7) 		644.7
(3) 		539
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 139.9
(3)
P 		20.4
(-2.4)
A 		7
(-3)
A 		116.6
(1.9)
P 		133
B 98.5
(0.5)
P 		25.7
(-2.4)
A 		10.1
(-3)
P 		161.9
(3)
P 		152
C 112.8
(1.4)
P 		18.5
(-2.9)
P 		16.6
(-3)
A 		148.2
(3)
P 		132
avr 117.1
(1.9) 		21.5
(-2.5) 		11.2
(-3) 		142.2
(3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 240
1 2 3 4 5 6 range
A 67.9
(-1.2)
P 		72.4
(-1)
P 		42.2
(-2.5)
P 		30.8
(-3)
P 		91.2
(-0.1)
P 		157.6
(3)
P 		127
B 169
(1)
P 		58.7
(-2.1)
P 		32.9
(-2.9)
P 		27.9
(-3)
P 		126.8
(-0.2)
P 		239.8
(3)
P 		212
C 258.4
(3)
P 		70.7
(-1.7)
P 		30.7
(-2.7)
P 		18
(-3)
P 		102.5
(-0.9)
P 		215.5
(1.9)
P 		240
avr 165.1
(1.7) 		67.3
(-1.6) 		35.3
(-2.7) 		25.6
(-3) 		106.8
(-0.3) 		204.3
(3) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193823_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9210
Sequence Type Exemplar sequence
Representative Public ID R05A10.1 NCBI
Target Description R05A10.1 /REP_DB=WormBase Gene ID /WP=CE28066 /TR=O45699 /GB=CAB05263.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Reverse transcriptase (RNA-dependent DNA polymerase)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070320
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070320 NCBI Caenorhabditis elegans R05A10.1 (R05A10.1) mRNA, complete cds. A 
R05A10.1 ENSEMBL cdna:known chromosome:CEL140:IV:14182346:14192643:1 gene:R05A10.1 A 
GENEFINDER00000012484 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14182348:14184404:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14182056-14184424(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05A10.1
Entrez Gene 178372 Entrez gene
SwissProt O45699 EMBL-EBI
WormBase CE36884 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05263 Hypothetical protein R05A10.1 [Caenorhabditis elegans] emb|CAC42314.2| Hypothetical protein R05A10.1 [Caenorhabditis elegans] ref|NP_502721.2| R05A10.1 [Caenorhabditis elegans] 0.0
blast CAB05263 Hypothetical protein R05A10.1 [Caenorhabditis elegans] emb|CAC42314.2| Hypothetical protein R05A10.1 [Caenorhabditis elegans] ref|NP_502721.2| R05A10.1 [Caenorhabditis elegans] 1.0E-111
blast T23192 hypothetical protein K01G12.2 - Caenorhabditis elegans 4.0E-99
blast CAE71759 Hypothetical protein CBG18749 [Caenorhabditis briggsae] 5.0E-69
blast CAE71759 Hypothetical protein CBG18749 [Caenorhabditis briggsae] 1.0E-68
blast CAE74599 Hypothetical protein CBG22381 [Caenorhabditis briggsae] 2.0E-43
Sequence
Target Sequence 
>C. ELEGANS:193823_AT
gaaacataatgattctccactccctgattcttgtttctgcggtgtctttggtctcctcac
tggacactacaaaaaccatgacgtcacagctacacgagttccaaatgaacaactcggccg
gtgtaatgtctgcattggaaatgttaccaaaggtatggtcaacgggggaatttctcaata
tcacaacagtttcatgaatagcatcattggtgcatggcgtggctttgttaaaaaaaagta
ttaccttcagacatgtggagtgatgggaatgccagaatgtgacaaagttccattgccaaa
aggatttgaagcagcaatgaagaaacacggagagaaaaatgaagttccgggaaaaaccaa
tgttgatggtgtggacttgccaaagggatggtcattggttgatggtggaaaaaagccaat
aactggaacaaatgcatcagttgttactcatattctgatgttcaatccaacacaactcag
agcaaaaagaggtggattcaagggggcgggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACATAATGATTCTCCACTCCCT 4 357 24 Antisense
AAAACCATGACGTCACAGCTACACG 551 131 95 Antisense
GTCACAGCTACACGAGTTCCAAATG 370 465 106 Antisense
CAACTCGGCCGGTGTAATGTCTGCA 559 177 133 Antisense
TATGGTCAACGGGGGAATTTCTCAA 565 651 177 Antisense
GCATCATTGGTGCATGGCGTGGCTT 114 309 224 Antisense
GTATTACCTTCAGACATGTGGAGTG 469 451 261 Antisense
GATGGTGTGGACTTGCCAAAGGGAT 566 407 388 Antisense
GTTGTTACTCATATTCTGATGTTCA 626 437 463 Antisense
TTCAATCCAACACAACTCAGAGCAA 408 685 484 Antisense
GAGGTGGATTCAAGGGGGCGGGAGT 65 403 512 Antisense
Sequence Source Affymetrix Proprietary Database